##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512090_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2199630 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.052032387265132 31.0 31.0 34.0 28.0 34.0 2 31.243110432209054 31.0 31.0 34.0 28.0 34.0 3 31.347047912603482 33.0 31.0 34.0 28.0 34.0 4 34.93520955797111 37.0 35.0 37.0 32.0 37.0 5 34.5719098211972 37.0 35.0 37.0 31.0 37.0 6 34.52765919722862 37.0 35.0 37.0 31.0 37.0 7 34.36753453989989 37.0 35.0 37.0 30.0 37.0 8 34.27674927146838 37.0 35.0 37.0 30.0 37.0 9 35.80859008105909 39.0 35.0 39.0 30.0 39.0 10 35.627322777012495 39.0 35.0 39.0 30.0 39.0 11 35.61778799161677 39.0 35.0 39.0 30.0 39.0 12 35.45958411187336 39.0 35.0 39.0 30.0 39.0 13 35.43216040879602 39.0 35.0 39.0 30.0 39.0 14 36.48502248105363 40.0 36.0 41.0 30.0 41.0 15 36.423358019303244 40.0 36.0 41.0 30.0 41.0 16 36.326318062583255 40.0 36.0 41.0 29.0 41.0 17 36.270948295849756 40.0 36.0 41.0 29.0 41.0 18 36.21187199665398 39.0 36.0 41.0 29.0 41.0 19 36.23335015434414 40.0 36.0 41.0 29.0 41.0 20 36.13437714524716 39.0 36.0 41.0 29.0 41.0 21 36.012820337965934 39.0 36.0 41.0 28.0 41.0 22 35.912297977387105 39.0 36.0 41.0 27.0 41.0 23 35.976588789932855 39.0 36.0 41.0 29.0 41.0 24 35.87517900737851 39.0 36.0 41.0 28.0 41.0 25 35.46399348981419 39.0 35.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2341608943917528 1101 2 0.18204708642004164 1101 3 0.1170686153078897 1101 4 0.14800677595695788 1101 5 0.21767034564849297 1101 6 0.18028059440167965 1101 7 0.20994771708107152 1101 8 0.16210961626862996 1101 9 0.31171928045789343 1101 10 0.2887565001311785 1101 11 0.23545181457212294 1101 12 0.2286838545393124 1101 13 0.22047301873954694 1101 14 0.22148538169980014 1101 15 0.31174398867222664 1101 16 0.1614639181388 1101 17 0.01987129766553153 1101 18 0.27525891323632834 1101 19 -0.013072672560170417 1101 20 0.041512673947593726 1101 21 0.05796849704300655 1101 22 -0.06792946442097048 1101 23 0.03430338493535601 1101 24 -0.2617958513708132 1101 25 -0.20606291505698238 1102 1 0.22722337687984506 1102 2 0.20083105952175018 1102 3 0.31228296106300135 1102 4 0.35302779024667785 1102 5 0.08859777630134147 1102 6 0.1677056434350206 1102 7 0.2899813399446174 1102 8 0.29685324193408036 1102 9 0.3614138727806733 1102 10 0.31066179573218733 1102 11 0.324625252509918 1102 12 0.4684092678203413 1102 13 0.5132328954557153 1102 14 0.47701354084802006 1102 15 0.29273025933628105 1102 16 0.32807641463642057 1102 17 0.4010817207532327 1102 18 0.30861279387516305 1102 19 0.37908199261215003 1102 20 0.668450191459506 1102 21 0.6780077237171511 1102 22 0.6376014966325485 1102 23 0.7739559486786973 1102 24 0.5614935854462217 1102 25 0.5118692788348653 1103 1 0.14398223737686422 1103 2 0.1879009545675281 1103 3 0.24043770208021797 1103 4 0.19635952853104044 1103 5 0.2870093202024009 1103 6 0.2448924024018595 1103 7 0.36308349751418945 1103 8 0.34856451680355605 1103 9 0.2265948045019428 1103 10 0.19804346968290787 1103 11 0.16984574144559872 1103 12 0.37155768180998905 1103 13 0.3496397818137922 1103 14 0.4439098945660831 1103 15 0.4610596050471969 1103 16 0.5062667784139165 1103 17 0.5322472762024546 1103 18 0.48985799543618214 1103 19 0.45872640302888357 1103 20 0.367978482311365 1103 21 0.6515575583637414 1103 22 0.4676343546412909 1103 23 0.31226307004065745 1103 24 0.6528945701231166 1103 25 0.26969427006209656 1104 1 0.1848432617061917 1104 2 0.23951298441673785 1104 3 0.21085013341290448 1104 4 0.213801580344267 1104 5 0.1660161598696206 1104 6 0.13340663409690023 1104 7 0.16002882012611508 1104 8 0.08321200681769625 1104 9 0.06765114735508604 1104 10 -0.10911333139047485 1104 11 0.37369154579216257 1104 12 0.25269406468728306 1104 13 0.1075961241080563 1104 14 0.1967783507693781 1104 15 0.31679692108999546 1104 16 0.2831060502707672 1104 17 0.33467873929261316 1104 18 0.26113640236895463 1104 19 0.3523823389753318 1104 20 0.2978561101641688 1104 21 0.35487087341131485 1104 22 0.16379813406714305 1104 23 0.30098154165457913 1104 24 0.05797289314096332 1104 25 0.09402620557220587 1105 1 0.050930553674042756 1105 2 0.10437402859735556 1105 3 -0.009707953332263486 1105 4 0.12418266229801844 1105 5 0.1295613505282276 1105 6 0.13990619357255696 1105 7 0.22963917106906706 1105 8 0.14248423301518187 1105 9 0.09699651844209001 1105 10 0.11208465144611779 1105 11 0.04385380964920671 1105 12 -0.13515537333994843 1105 13 0.12914145592916526 1105 14 0.25270121276599866 1105 15 0.3229561491420654 1105 16 0.23082501845404124 1105 17 0.188745720086402 1105 18 0.03903790015173314 1105 19 0.17353310539034084 1105 20 0.1841336956688906 1105 21 0.1222291957518209 1105 22 0.1232053976077907 1105 23 0.13880591116932095 1105 24 0.16637230512614565 1105 25 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11 -0.03313971970658258 1107 12 0.11508965473122856 1107 13 0.07988446985373798 1107 14 0.04373508849156593 1107 15 0.09986958841633964 1107 16 0.03285105962510215 1107 17 0.08749044682855356 1107 18 0.09963358004515754 1107 19 0.02518407122914823 1107 20 0.18103714350725397 1107 21 0.07809004518247065 1107 22 -0.002915070562345079 1107 23 0.08484502050417575 1107 24 0.07527419874114116 1107 25 0.11678387031459181 1108 1 -0.06101410365659632 1108 2 -0.04929903239647615 1108 3 -0.07521013251276898 1108 4 -0.051671591942287876 1108 5 -0.08842869413916787 1108 6 -0.08102206765041586 1108 7 -0.005071148879530085 1108 8 -0.09135209233685515 1108 9 -0.14638059203423381 1108 10 -0.18281653065286463 1108 11 -0.1908491560490191 1108 12 -0.009454010094167131 1108 13 -0.11465097616014219 1108 14 -0.12312207620959015 1108 15 0.09281765699563493 1108 16 -0.04030974237799967 1108 17 -0.04452277972394114 1108 18 -0.2321818216253675 1108 19 -0.03526528751241642 1108 20 -0.04767517897597884 1108 21 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-0.42284519478180016 2314 18 -0.46727263138186004 2314 19 -0.6641661243816372 2314 20 -0.741897088031763 2314 21 -0.5302371862100941 2314 22 -0.4860182735582228 2314 23 -0.37308750249142264 2314 24 -0.4173804593061021 2314 25 -0.6574282819619555 2315 1 -0.5827173066716078 2315 2 -0.6074412312229995 2315 3 -0.6809568468646887 2315 4 -0.6245487091413295 2315 5 -0.7437325127486574 2315 6 -0.7078126693415498 2315 7 -0.7753232453110073 2315 8 -0.8446324938723393 2315 9 -1.01681981519598 2315 10 -0.8497694254334291 2315 11 -0.74260193890332 2315 12 -0.9810229890292916 2315 13 -1.0715576139125176 2315 14 -0.9690555675221901 2315 15 -1.1891866976357548 2315 16 -1.3491667828026195 2315 17 -1.2082639152509103 2315 18 -1.3056815286933343 2315 19 -1.2792138364058374 2315 20 -1.3992609468513848 2315 21 -1.3062781250729856 2315 22 -1.4513995390514722 2315 23 -1.3120397202790244 2315 24 -1.242532400807427 2315 25 -1.2357043959603544 2316 1 -1.0085241314258013 2316 2 -1.0718599703785792 2316 3 -1.187739187331239 2316 4 -0.9354860524997264 2316 5 -1.2199337851604568 2316 6 -1.248010857118686 2316 7 -1.2930884274972456 2316 8 -1.397715971797922 2316 9 -1.5132262158129066 2316 10 -1.780815502163712 2316 11 -1.5933858216871997 2316 12 -1.6527114409282717 2316 13 -1.513934324920811 2316 14 -1.7733740560512032 2316 15 -1.6207448237201945 2316 16 -1.8908826108342325 2316 17 -1.9035251452489845 2316 18 -1.9522155560694685 2316 19 -1.7137817947632712 2316 20 -1.8331356335681832 2316 21 -1.9123355763935947 2316 22 -1.8788990570773052 2316 23 -1.8133707401363637 2316 24 -1.6787367578538905 2316 25 -1.802863833014527 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 27795.0 3 3.0 4 452.0 5 4662.0 6 5999.0 7 4872.0 8 3960.0 9 3536.0 10 3398.0 11 3284.0 12 3127.0 13 3070.0 14 3081.0 15 3028.0 16 3121.0 17 3222.0 18 3466.0 19 4101.0 20 5031.0 21 6276.0 22 8270.0 23 10434.0 24 12989.0 25 15449.0 26 18290.0 27 20691.0 28 24489.0 29 30166.0 30 38241.0 31 50012.0 32 64690.0 33 84481.0 34 110386.0 35 148105.0 36 215543.0 37 376552.0 38 875358.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.20228404655759 20.165851805448764 13.727245829962175 24.904618318031478 2 14.613548642271654 21.352091033491995 40.75485422548338 23.27950609875297 3 17.790673886062656 25.808658728968055 31.08859217231989 25.312075212649397 4 12.436864381736928 18.21306310606784 38.801980333056015 30.548092179139218 5 12.713694312568771 38.28072938202127 36.337141996780396 12.668434308629559 6 30.437664516305013 38.35836026968172 18.588035260475625 12.61593995353764 7 26.82260198306079 32.33430167800949 23.290462486872794 17.552633852056935 8 24.551604114089933 37.23557815802502 21.209349311658727 17.003468416226315 9 26.100207762214556 15.299664034405785 20.993576192359626 37.606552011020035 10 14.7950337102149 29.327295954319588 35.02193550733532 20.855734828130185 11 33.29723635338671 22.818974100189575 25.41495615171642 18.468833394707293 12 22.54142742188459 25.268658819892437 32.538108681914686 19.65180507630829 13 28.415142546701038 22.462004973563733 26.35984233712033 22.763010142614895 14 20.573914703836554 22.557339188863583 28.428326582197915 28.44041952510195 15 22.475052622486512 31.243436396121165 24.082732095852485 22.19877888553984 16 21.62377308911044 28.485427094556815 28.34299404899915 21.547805767333596 17 21.135736464769074 28.908543709623892 28.479380623104795 21.47633920250224 18 20.92974727567818 27.826861790392023 30.443347290226086 20.800043643703713 19 22.922295461912405 27.33249773711634 28.36339979460154 21.381807006369716 20 23.0023685801703 27.69874933511545 28.718011665598304 20.580870419115943 21 22.48500884239622 27.14079186044926 28.01098366543464 22.36321563171988 22 22.571568854761935 28.273982442501694 28.23279369712179 20.921655005614582 23 21.84308270027232 28.018985011115504 28.491428103817462 21.646504184794715 24 22.392927142309617 27.27138399650467 29.095474576802893 21.24021428438282 25 22.22748580310702 27.651742293261368 28.7197996363886 21.400972267243013 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1218.0 1 1218.0 2 1050.5 3 883.0 4 883.0 5 883.0 6 2152.0 7 3421.0 8 3421.0 9 3421.0 10 4754.0 11 6087.0 12 6087.0 13 6087.0 14 9579.0 15 13071.0 16 13071.0 17 13071.0 18 22610.0 19 32149.0 20 32149.0 21 32149.0 22 49567.5 23 66986.0 24 66986.0 25 66986.0 26 93299.5 27 119613.0 28 119613.0 29 119613.0 30 150971.5 31 182330.0 32 182330.0 33 182330.0 34 203518.0 35 224706.0 36 224706.0 37 224706.0 38 238493.0 39 252280.0 40 252280.0 41 252280.0 42 263848.0 43 275416.0 44 275416.0 45 275416.0 46 277040.0 47 278664.0 48 278664.0 49 278664.0 50 266328.0 51 253992.0 52 253992.0 53 253992.0 54 225194.5 55 196397.0 56 196397.0 57 196397.0 58 166491.5 59 136586.0 60 136586.0 61 136586.0 62 109837.5 63 83089.0 64 83089.0 65 83089.0 66 63218.5 67 43348.0 68 43348.0 69 43348.0 70 31193.5 71 19039.0 72 19039.0 73 19039.0 74 13074.5 75 7110.0 76 7110.0 77 7110.0 78 4722.0 79 2334.0 80 2334.0 81 2334.0 82 1508.0 83 682.0 84 682.0 85 682.0 86 423.5 87 165.0 88 165.0 89 165.0 90 105.0 91 45.0 92 45.0 93 45.0 94 32.0 95 19.0 96 19.0 97 19.0 98 9.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4310724985565754 2 0.0 3 0.0 4 0.0 5 0.05550933566099753 6 0.0 7 0.0 8 0.0030459668216927392 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 1.3638657410564503E-4 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0055918495383314466 25 0.001318403549687902 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2199630.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.64673402489228 #Duplication Level Percentage of deduplicated Percentage of total 1 80.64052370771913 56.16349106299534 2 13.138243050144421 18.300714385355956 3 3.473429580330768 7.257390784064706 4 1.2490182990848924 3.479601810743554 5 0.5379303651670374 1.8732546533350918 6 0.2800862027685594 1.170425356095834 7 0.1543311284727655 0.7524061339552925 8 0.1062086559287184 0.5917668808487 9 0.0771939165596947 0.48386737574755057 >10 0.30558648414959727 3.6192516924988585 >50 0.020028655606414456 0.9613100368285092 >100 0.013964523610898779 1.989638408064949 >500 0.0022166912366589075 1.1170230889359665 >1k 0.00104314881725117 1.460076318078613 >5k 1.9559040323459436E-4 0.7797820124510657 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 7081 0.32191777708069086 No Hit TATCAACGCAGAGTACTTTTTTTTT 5090 0.23140255406591106 No Hit GGTATCAACGCAGAGTACTTTTTTT 5002 0.22740188122547883 No Hit CTTTAGGACGTGAAATATGGCGAGG 3582 0.16284556948214018 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3574 0.1624818719511918 No Hit GTCCTACAGTGGACATTTCTAAATT 3474 0.15793565281433697 No Hit CTGTAGGACGTGGAATATGGCAAGA 3438 0.1562990139250692 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2475 0.11251892363715715 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 4.546219136854835E-5 0.0 12 0.0 0.0 0.0 4.546219136854835E-5 0.0 13 0.0 0.0 0.0 4.546219136854835E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCGAGG 915 0.0 11.936062 19 GCGCCAC 120 1.0075382E-8 11.870652 13 GTGCGCC 105 2.734614E-7 11.757599 11 GGTATCA 1510 0.0 11.686347 1 TAGACGA 75 2.0697933E-4 11.402824 5 AGGACCG 85 5.311535E-5 11.17924 5 GGACGTA 60 0.005893013 11.079276 6 TGCGCCA 140 8.403731E-9 10.853168 12 TAGGACC 510 0.0 10.806599 4 CGTGCGC 110 6.074224E-6 10.359842 10 TGTACGG 75 0.0026428814 10.135843 5 GAGGCGT 75 0.0026563758 10.129623 6 TTAGGAC 1380 0.0 10.053215 3 AGGACGT 2085 0.0 9.98089 5 GTCTAGG 220 1.8189894E-12 9.972159 1 GTATAAA 335 0.0 9.965687 1 TGTAGGA 1970 0.0 9.888237 2 GGCGTGC 125 2.5828704E-6 9.876383 8 GGACGTG 2035 0.0 9.79985 6 TGGCGAG 1950 0.0 9.643061 18 >>END_MODULE