FastQCFastQC Report
Thu 26 May 2016
SRR1512089_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512089_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3313673
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT63660.19211310228860845No Hit
TATCAACGCAGAGTACTTTTTTTTT39470.11911253765836279No Hit
GGTATCAACGCAGAGTACTTTTTTT38210.11531011056311229No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGT1251.9665094E-811.34645756
TAAGACG600.00608761211.0312794
GGTATCA18900.010.8390251
TAGGACC5650.09.8737624
TTAGGAC9400.09.7571493
GTCAACG950.00125679759.3756312
GTCCTAC13000.09.3636121
CTTATAC2750.09.2834653
GTATCAA34250.09.2752591
GTCTTAT3100.09.2580171
TCCAACG2252.910383E-119.24540118
ATAATAC3900.09.2129363
GGACGTG12850.09.1978426
AGGACGT13250.08.9915335
TGGCGAG14600.08.93741318
ATCGCTC850.0077312318.8991828
GTCTTAG4450.08.895731
TAGGACA5850.08.8896754
ACCGTCC1502.8816085E-68.8250238
GTATAGA5050.08.8186641
Produced by FastQC (version 0.11.5)