##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512089_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3313673 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.31797977651989 31.0 28.0 33.0 16.0 34.0 2 25.80872131921285 31.0 25.0 33.0 7.0 34.0 3 27.455331289478472 31.0 26.0 34.0 16.0 34.0 4 31.67191964928344 35.0 32.0 37.0 19.0 37.0 5 32.04307123847163 35.0 33.0 37.0 19.0 37.0 6 32.28231029434709 35.0 33.0 37.0 22.0 37.0 7 32.302391032549075 35.0 33.0 37.0 22.0 37.0 8 32.336312907157705 36.0 33.0 37.0 22.0 37.0 9 33.816366008353874 38.0 34.0 39.0 23.0 39.0 10 33.65588547813861 38.0 34.0 39.0 22.0 39.0 11 33.667321730297466 38.0 34.0 39.0 22.0 39.0 12 33.54452295081621 38.0 34.0 39.0 20.0 39.0 13 33.5266554666076 38.0 34.0 39.0 19.0 39.0 14 34.52294538417038 39.0 34.0 41.0 19.0 41.0 15 34.43631432552337 39.0 34.0 41.0 18.0 41.0 16 34.38963802402953 39.0 34.0 41.0 18.0 41.0 17 34.30712082936367 39.0 34.0 41.0 17.0 41.0 18 34.252189941493924 39.0 34.0 41.0 17.0 41.0 19 34.252276250553386 39.0 34.0 41.0 18.0 41.0 20 34.17040245069444 39.0 34.0 41.0 18.0 41.0 21 34.09106993961082 39.0 34.0 41.0 16.0 41.0 22 33.95846814094209 39.0 34.0 41.0 10.0 41.0 23 33.82485356883434 39.0 34.0 41.0 8.0 41.0 24 33.682891160352874 39.0 34.0 41.0 2.0 41.0 25 33.535616519795404 39.0 33.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.1081023596873152 1101 2 3.527893402075577 1101 3 1.8718275283357713 1101 4 1.345104109239795 1101 5 1.0175558133418576 1101 6 0.7230612387581843 1101 7 0.6104737568704692 1101 8 0.5423453564082976 1101 9 0.5066873335380322 1101 10 0.2650073295367079 1101 11 0.5085187690039561 1101 12 0.22632644666251167 1101 13 0.21386023987909653 1101 14 0.3349586954793722 1101 15 0.4957448895043939 1101 16 0.2951416406856566 1101 17 0.2751041996391663 1101 18 0.23341781266313433 1101 19 0.310343462497336 1101 20 0.2594766333609755 1101 21 0.3463179182386966 1101 22 0.2349556974095819 1101 23 0.2430108839672016 1101 24 0.14572465125631595 1101 25 0.09498423585221616 1102 1 1.3570160714810307 1102 2 3.7145935421600065 1102 3 2.428038783264082 1102 4 1.4968762406782936 1102 5 1.2650488725069238 1102 6 1.0961885602931503 1102 7 0.9758326519148994 1102 8 0.9130635902035067 1102 9 0.7268144078651844 1102 10 0.7703658759547309 1102 11 0.9158483223818337 1102 12 0.7827488582698692 1102 13 0.8994150629670372 1102 14 0.9166062088222517 1102 15 0.9204752586680698 1102 16 1.004610254800589 1102 17 0.7524949844923015 1102 18 0.8023586856236662 1102 19 0.838884080643659 1102 20 0.9217879789321515 1102 21 0.9004425648582881 1102 22 0.8330480770700888 1102 23 0.6539023692424877 1102 24 0.7128118617379258 1102 25 0.7084241009654946 1103 1 1.1569581925588075 1103 2 2.963015725796282 1103 3 1.9257882783628446 1103 4 1.2651696744992869 1103 5 1.0299434589804974 1103 6 0.9419643225708754 1103 7 0.8148030228325922 1103 8 0.6663516055722098 1103 9 0.9072811952155604 1103 10 0.7447421382078261 1103 11 0.8002228605819184 1103 12 0.747047107124601 1103 13 0.8302620655777488 1103 14 0.8935931799074197 1103 15 0.6532301106458078 1103 16 0.6732057057635998 1103 17 0.7291340663109338 1103 18 0.8887152690432032 1103 19 0.9266784011204834 1103 20 1.0605819422837115 1103 21 1.0129662705870714 1103 22 0.964442466509027 1103 23 1.104306598098212 1103 24 0.8717587732201153 1103 25 0.9157722611546433 1104 1 1.3822135646346005 1104 2 3.8627376707825967 1104 3 2.382403946224148 1104 4 1.758003945394048 1104 5 1.4509482375194196 1104 6 1.0811645977905187 1104 7 0.9674461521707016 1104 8 0.9293001606690652 1104 9 0.9456573236917194 1104 10 1.0729408104998583 1104 11 1.0633290620971394 1104 12 1.0409816895317192 1104 13 0.9460520366948018 1104 14 1.0676809477551146 1104 15 0.8612940840916465 1104 16 1.084819748014354 1104 17 1.044962031348959 1104 18 1.0582112541631972 1104 19 0.9897940538598249 1104 20 1.0371192783994871 1104 21 1.0681701145889448 1104 22 1.0524490521220002 1104 23 1.150615895078353 1104 24 1.2690419243338482 1104 25 1.180000133948731 1105 1 1.2237863144198116 1105 2 3.6496087405325746 1105 3 2.0940185344458513 1105 4 1.4607808407582503 1105 5 1.0081728583021814 1105 6 0.7830572545853869 1105 7 0.471202586770616 1105 8 0.569868463089847 1105 9 0.6571172288242408 1105 10 0.7063666167651519 1105 11 0.4511029612661446 1105 12 0.4274766104393848 1105 13 0.6201295334639667 1105 14 0.5042202701096201 1105 15 0.6664412225447478 1105 16 0.5686763927089231 1105 17 0.6705751684014274 1105 18 0.598188981242302 1105 19 0.7691259023317656 1105 20 0.9713509889742511 1105 21 0.8854639366510497 1105 22 0.9692425456855958 1105 23 0.9551939369384499 1105 24 0.8725586633183227 1105 25 0.8910044730169489 1106 1 0.7853673781184547 1106 2 3.220401727212252 1106 3 1.809599862746147 1106 4 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-2.3077743351979265 2315 20 -2.3612876980235704 2315 21 -2.475273649534394 2315 22 -2.622420619184897 2315 23 -2.399795519587016 2315 24 -2.492064647091471 2315 25 -2.4636790259693306 2316 1 -1.8523914122848026 2316 2 -2.1049237228457933 2316 3 -1.5032443949290482 2316 4 -2.012180017191884 2316 5 -2.0690996670353066 2316 6 -2.183636166256811 2316 7 -2.28286651600688 2316 8 -2.4224534340674957 2316 9 -2.533574687465798 2316 10 -2.757878797016307 2316 11 -3.023172010449457 2316 12 -2.879010882230368 2316 13 -3.0493985465734674 2316 14 -3.4166901912666354 2316 15 -3.451342258183363 2316 16 -3.2929569348984273 2316 17 -3.2746848999549982 2316 18 -3.203319569234239 2316 19 -3.308273088315133 2316 20 -3.425050525055404 2316 21 -3.4737736581979313 2316 22 -3.2586144744718517 2316 23 -3.3671231918547697 2316 24 -3.2736719596491994 2316 25 -3.547670810271267 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 179234.0 3 6.0 4 1514.0 5 7308.0 6 8612.0 7 7791.0 8 7210.0 9 6897.0 10 6672.0 11 6432.0 12 6372.0 13 6335.0 14 6580.0 15 6726.0 16 7017.0 17 7608.0 18 8789.0 19 10667.0 20 14112.0 21 18100.0 22 22147.0 23 26213.0 24 30235.0 25 34322.0 26 38434.0 27 43226.0 28 49523.0 29 59353.0 30 73052.0 31 90378.0 32 113863.0 33 145556.0 34 189884.0 35 252551.0 36 355526.0 37 484856.0 38 980572.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.98363990653272 18.21763945929487 12.747968794748305 25.050751839424105 2 16.61388536697691 21.31764361030407 38.15178271919401 23.916688303525007 3 18.466517366076857 24.719125876331187 29.649877945108045 27.16447881248391 4 12.942888450369122 16.958855022810035 37.40942452680153 32.68883200001931 5 13.933390530689058 37.39080470523193 34.96992612125578 13.705878642823235 6 32.10950507186436 36.49989000121617 18.077583394619808 13.313021532299658 7 28.27786567956464 31.641082267320886 22.156048590189798 17.92500346292468 8 25.92166075931031 35.439641605292024 20.568132264426247 18.070565370971416 9 26.78966814166636 15.496067354865733 19.832192253128174 37.882072250339725 10 15.72867932351804 28.450634688455985 33.477654554326875 22.3430314336991 11 34.38254166901803 22.559045506300713 24.0325161837031 19.025896640978154 12 23.41145309147885 24.49867563878512 30.89686278640047 21.19300848333556 13 28.85173039101927 21.489809042714835 25.73763313398757 23.920827432278323 14 22.121997392008417 21.09651530437181 27.14628925529799 29.635198048321783 15 23.739608585397534 29.062523670863115 23.466618462352802 23.731249281386546 16 23.376597509772388 27.55425776773991 26.155507800558475 22.913636921929232 17 22.683771150623492 27.720085838282778 26.876429871022278 22.719713140071455 18 22.576488386150352 27.20165809963747 28.032850555863536 22.189002958348635 19 23.930769144877864 26.694987758275047 26.948634247195024 22.42560884965206 20 23.972572975067493 26.676285419230545 27.206604407324093 22.14453719837787 21 23.957199143427594 26.217652199734914 26.744741181384278 23.080407475453214 22 24.037022562698894 26.487277643568806 26.81498224769929 22.660717546033005 23 23.65043486658257 26.478455846405485 26.801784129934873 23.06932515707707 24 23.625328337453645 26.305865652039557 27.10135970333745 22.967446307169347 25 23.623121533114464 26.40408392741227 26.97248642216658 23.000308117306687 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 623.0 1 623.0 2 764.5 3 906.0 4 906.0 5 906.0 6 2124.5 7 3343.0 8 3343.0 9 3343.0 10 4762.5 11 6182.0 12 6182.0 13 6182.0 14 10261.0 15 14340.0 16 14340.0 17 14340.0 18 25080.0 19 35820.0 20 35820.0 21 35820.0 22 54179.5 23 72539.0 24 72539.0 25 72539.0 26 102594.0 27 132649.0 28 132649.0 29 132649.0 30 171340.5 31 210032.0 32 210032.0 33 210032.0 34 243021.5 35 276011.0 36 276011.0 37 276011.0 38 308540.5 39 341070.0 40 341070.0 41 341070.0 42 370015.0 43 398960.0 44 398960.0 45 398960.0 46 415186.5 47 431413.0 48 431413.0 49 431413.0 50 429286.5 51 427160.0 52 427160.0 53 427160.0 54 392955.0 55 358750.0 56 358750.0 57 358750.0 58 311018.5 59 263287.0 60 263287.0 61 263287.0 62 217224.0 63 171161.0 64 171161.0 65 171161.0 66 134103.5 67 97046.0 68 97046.0 69 97046.0 70 71101.5 71 45157.0 72 45157.0 73 45157.0 74 31667.0 75 18177.0 76 18177.0 77 18177.0 78 12256.0 79 6335.0 80 6335.0 81 6335.0 82 4156.5 83 1978.0 84 1978.0 85 1978.0 86 1255.5 87 533.0 88 533.0 89 533.0 90 337.0 91 141.0 92 141.0 93 141.0 94 94.0 95 47.0 96 47.0 97 47.0 98 30.0 99 13.0 100 13.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.459673600865263 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 2.4142394255558712E-4 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 3.621359138333807E-4 15 0.0 16 0.0 17 0.0 18 0.0 19 1.8106795691669034E-4 20 2.112459497361387E-4 21 3.017799281944839E-5 22 5.432038707500709E-4 23 3.923139066528291E-4 24 2.7160193537503546E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3313673.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.27609194110951 #Duplication Level Percentage of deduplicated Percentage of total 1 81.18054305571518 56.23870764556924 2 11.941983673554972 16.545879178568462 3 3.39946408444815 7.065047593941892 4 1.3440243696167091 3.724350232026356 5 0.6651127214737546 2.3038205022008698 6 0.3778899364044541 1.5707242786784998 7 0.23963538476830218 1.162070206328644 8 0.17801176362197293 0.9865567442619882 9 0.11745445553704105 0.7323107094609315 >10 0.5278314332770182 6.109783627688657 >50 0.018188914586921182 0.8611363479861257 >100 0.008060267806237049 1.0830778445621236 >500 0.0010536229446363467 0.5068608344607145 >1k 7.024152891092233E-4 0.9160659471244981 >5k 4.3900955569326435E-5 0.19360830714101632 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6366 0.19211310228860845 No Hit TATCAACGCAGAGTACTTTTTTTTT 3947 0.11911253765836279 No Hit GGTATCAACGCAGAGTACTTTTTTT 3821 0.11531011056311229 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 3.0177992819448387E-5 0.0 0.0 0.0 3 0.0 3.0177992819448387E-5 0.0 3.0177992819448387E-5 0.0 4 0.0 3.0177992819448387E-5 0.0 3.0177992819448387E-5 0.0 5 0.0 3.0177992819448387E-5 0.0 3.0177992819448387E-5 0.0 6 0.0 3.0177992819448387E-5 0.0 3.0177992819448387E-5 0.0 7 0.0 3.0177992819448387E-5 0.0 3.0177992819448387E-5 3.0177992819448387E-5 8 0.0 3.0177992819448387E-5 0.0 3.0177992819448387E-5 3.0177992819448387E-5 9 0.0 3.0177992819448387E-5 0.0 3.0177992819448387E-5 3.0177992819448387E-5 10 0.0 3.0177992819448387E-5 0.0 3.0177992819448387E-5 3.0177992819448387E-5 11 0.0 3.0177992819448387E-5 0.0 3.0177992819448387E-5 3.0177992819448387E-5 12 0.0 3.0177992819448387E-5 0.0 3.0177992819448387E-5 9.053397845834517E-5 13 0.0 3.0177992819448387E-5 0.0 3.0177992819448387E-5 9.053397845834517E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATACCGT 125 1.9665094E-8 11.3464575 6 TAAGACG 60 0.006087612 11.031279 4 GGTATCA 1890 0.0 10.839025 1 TAGGACC 565 0.0 9.873762 4 TTAGGAC 940 0.0 9.757149 3 GTCAACG 95 0.0012567975 9.375631 2 GTCCTAC 1300 0.0 9.363612 1 CTTATAC 275 0.0 9.283465 3 GTATCAA 3425 0.0 9.275259 1 GTCTTAT 310 0.0 9.258017 1 TCCAACG 225 2.910383E-11 9.245401 18 ATAATAC 390 0.0 9.212936 3 GGACGTG 1285 0.0 9.197842 6 AGGACGT 1325 0.0 8.991533 5 TGGCGAG 1460 0.0 8.937413 18 ATCGCTC 85 0.007731231 8.899182 8 GTCTTAG 445 0.0 8.89573 1 TAGGACA 585 0.0 8.889675 4 ACCGTCC 150 2.8816085E-6 8.825023 8 GTATAGA 505 0.0 8.818664 1 >>END_MODULE