FastQCFastQC Report
Thu 26 May 2016
SRR1512089_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512089_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3313673
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT80780.24377782599550407No Hit
TATCAACGCAGAGTACTTTTTTTTT52920.15970193800052088No Hit
GGTATCAACGCAGAGTACTTTTTTT51410.15514506108478418No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22250.011.831711
GCACCGT1855.456968E-1210.780476
GGACGTG14000.010.582466
AGGACGT14350.010.46239955
TAGGACG15250.010.0941864
TTAGGAC10550.09.997623
GACGTGA8000.09.9719337
CGTGAAA9400.09.698569
TGGACCG1304.2293923E-69.5022425
AGCACCG2201.6370905E-119.5022415
TCCAACG1502.665165E-79.49650818
CGATCAC1002.7671538E-49.49650710
ACGTGAA9600.09.3981518
TAGACTG4050.09.384935
GACGTGG7500.09.3704527
TAGGACC5700.09.3355364
GTCCTAT3200.09.2401741
TACACCG1752.6624548E-89.230755
GTAGGAC14000.09.1628773
GTATCAA41050.09.1316211
Produced by FastQC (version 0.11.5)