FastQCFastQC Report
Thu 26 May 2016
SRR1512088_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512088_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2944681
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT67340.22868351444519797No Hit
TATCAACGCAGAGTACTTTTTTTTT42360.14385259388028787No Hit
GGTATCAACGCAGAGTACTTTTTTT41640.14140750729875323No Hit
GTCCTACAGTGGACATTTCTAAATT35000.11885837549126714No Hit
CTGTAGGACGTGGAATATGGCAAGA34710.11787354895148235No Hit
GTCCTAAAGTGTGTATTTCTCATTT32600.11070808688615166No Hit
CTTTAGGACGTGAAATATGGCGAGG31410.1066669021194486No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC6950.011.9716844
GGTATCA16300.011.6622311
GCACCGT658.3539373E-411.6368116
CGTTAGG700.001056476411.3151421
CGTTTAA700.001056476411.3151421
GGCGAGG10050.011.10128719
TAACGTG600.00608959911.0307285
TATAACG650.00783777610.6623452
GACCGTC909.9911296E-510.5054567
GTCCTAC18000.010.3957861
GTATAAG3750.010.2967781
TTAGGAC15450.010.1586743
AACCGTC750.002755374910.08523757
TGGCGAG21250.09.96658718
TTCAACG1104.3489224E-59.9007482
GTATCAA32150.09.8545561
AGGACGT23450.09.7169865
AATCCCG2151.0913936E-119.67479119
AGGACCT14500.09.5201165
TCCTACA20600.09.51625252
Produced by FastQC (version 0.11.5)