##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512088_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2944681 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.56552135868028 31.0 28.0 33.0 16.0 34.0 2 26.03359005610455 31.0 25.0 33.0 10.0 34.0 3 27.69055391738528 31.0 26.0 34.0 16.0 34.0 4 31.935895942548616 35.0 32.0 37.0 19.0 37.0 5 32.32609304709067 35.0 33.0 37.0 22.0 37.0 6 32.563764631890514 35.0 33.0 37.0 25.0 37.0 7 32.595729724204425 36.0 33.0 37.0 25.0 37.0 8 32.62367808261744 36.0 33.0 37.0 25.0 37.0 9 34.12084297076661 39.0 35.0 39.0 25.0 39.0 10 33.96979061568978 38.0 34.0 39.0 25.0 39.0 11 33.97928739989153 38.0 34.0 39.0 25.0 39.0 12 33.87307861191076 38.0 34.0 39.0 23.0 39.0 13 33.859426538901836 38.0 34.0 39.0 23.0 39.0 14 34.87831211598132 39.0 34.0 41.0 24.0 41.0 15 34.80118865167398 39.0 34.0 41.0 22.0 41.0 16 34.75974069856803 39.0 34.0 41.0 22.0 41.0 17 34.688646749851685 39.0 34.0 41.0 22.0 41.0 18 34.634391297393506 39.0 34.0 41.0 21.0 41.0 19 34.6479706969957 39.0 34.0 41.0 19.0 41.0 20 34.56803300595209 39.0 34.0 41.0 19.0 41.0 21 34.48914568335246 39.0 34.0 41.0 18.0 41.0 22 34.36752673719157 39.0 34.0 41.0 18.0 41.0 23 34.24687563780253 39.0 34.0 41.0 17.0 41.0 24 34.122942009677786 39.0 34.0 41.0 11.0 41.0 25 33.98832233440566 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.0238526488661215 1101 2 3.3031004958264916 1101 3 1.7520493211381947 1101 4 1.2396068143763017 1101 5 0.9069962315943059 1101 6 0.5743012454966134 1101 7 0.44657445559988673 1101 8 0.49583723749826447 1101 9 0.4123220132059515 1101 10 0.27309991962163593 1101 11 0.3821549351581126 1101 12 0.06182802816704225 1101 13 0.30006278684051324 1101 14 0.3017348082697566 1101 15 0.4212682776319667 1101 16 0.34862985523265877 1101 17 0.22395546516606402 1101 18 0.07019656884934733 1101 19 0.03664925246066986 1101 20 0.1081427879439616 1101 21 0.18983425438992896 1101 22 0.10078109121518963 1101 23 0.22254514768130207 1101 24 0.08200007666271603 1101 25 -0.04284263094258023 1102 1 1.092414999808657 1102 2 3.534574490403248 1102 3 2.176301583544678 1102 4 1.2546799954668302 1102 5 1.0729216946933917 1102 6 0.8792769062499843 1102 7 0.665517413553431 1102 8 0.5646793264263579 1102 9 0.49213922383027864 1102 10 0.5105770505308413 1102 11 0.6497620931835328 1102 12 0.6771740048405732 1102 13 0.5793168461041347 1102 14 0.40615466844160153 1102 15 0.501310095397983 1102 16 0.5273272971615057 1102 17 0.43101057967486867 1102 18 0.4608700635731182 1102 19 0.4093725527971728 1102 20 0.6030993366714625 1102 21 0.5252952299199976 1102 22 0.5201164991017606 1102 23 0.5121495124106303 1102 24 0.5466489544437607 1102 25 0.5903473621966882 1103 1 1.0585892698475838 1103 2 2.8373176044322186 1103 3 1.7761552867498445 1103 4 1.0574850080725255 1103 5 0.8623540356189636 1103 6 0.8160001036750018 1103 7 0.6649524662261754 1103 8 0.5167370365327244 1103 9 0.6503238613895519 1103 10 0.47400837848129385 1103 11 0.629683619543485 1103 12 0.7419676909892416 1103 13 0.7175515969476223 1103 14 0.7287382413453543 1103 15 0.6023947697033947 1103 16 0.49921207934729495 1103 17 0.7182166596871298 1103 18 0.6380801620802856 1103 19 0.6398229189812952 1103 20 0.7235920395170297 1103 21 0.8194710405457286 1103 22 0.7395910440489715 1103 23 0.6799313769136646 1103 24 0.500703139278805 1103 25 0.5831543545743259 1104 1 1.3201072125312727 1104 2 3.800830779177595 1104 3 2.4045262201075346 1104 4 1.6593105396194332 1104 5 1.3290484269999467 1104 6 1.0873581741903564 1104 7 0.7812829464402498 1104 8 0.8222200684245706 1104 9 0.8226278191572973 1104 10 0.9533202679140942 1104 11 0.8523840876895363 1104 12 0.9400872120383283 1104 13 0.8672173214274466 1104 14 0.971476900609261 1104 15 0.7291238396493469 1104 16 0.9269229636944232 1104 17 0.8846106136265348 1104 18 0.9821824497578788 1104 19 0.9634791610258233 1104 20 0.8948984662742276 1104 21 0.8395063166819412 1104 22 0.8355776244451363 1104 23 1.0408405985552633 1104 24 1.053751797124093 1104 25 0.8473749710735063 1105 1 1.0748963557183693 1105 2 3.5990308402941515 1105 3 2.1072260346956426 1105 4 1.4259583573271115 1105 5 0.9409301608354568 1105 6 0.6410905961495672 1105 7 0.4261991408284018 1105 8 0.4491121508306861 1105 9 0.569091237335158 1105 10 0.4394485901895848 1105 11 0.28939396007591256 1105 12 0.28244972790331957 1105 13 0.2781531780997426 1105 14 0.43055845090762546 1105 15 0.49785461183644486 1105 16 0.3702396993203152 1105 17 0.4446042020121368 1105 18 0.32035445068775203 1105 19 0.4797566002045386 1105 20 0.5443868025727738 1105 21 0.46275589439651554 1105 22 0.6107463745740276 1105 23 0.47237833615525915 1105 24 0.6023761355748434 1105 25 0.6566670547919458 1106 1 0.6855507897788122 1106 2 3.0675863100709826 1106 3 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0.4284724173197034 1107 15 0.3002559742349149 1107 16 0.5493834957742934 1107 17 0.46011952382950483 1107 18 0.3736448607344087 1107 19 0.41609085510115307 1107 20 0.5488513117906564 1107 21 0.5550753968636002 1107 22 0.5947749067454282 1107 23 0.719839931283559 1107 24 0.6823049958693943 1107 25 0.7711247377132509 1108 1 0.4895308966804919 1108 2 2.3381052284386463 1108 3 1.2503927883314248 1108 4 0.8514850781241137 1108 5 0.6004691390494443 1108 6 0.5547972403220385 1108 7 0.3805097032226783 1108 8 0.47612434297955986 1108 9 0.4651950834098457 1108 10 0.33117969306324113 1108 11 0.43888522653148243 1108 12 0.45099278851098035 1108 13 0.2866472777195668 1108 14 0.35390166177501925 1108 15 0.19641170233601457 1108 16 0.2708518877710304 1108 17 0.2403584949862605 1108 18 0.3086525280521428 1108 19 0.32580652029237456 1108 20 0.3119666247551507 1108 21 0.32516763798948034 1108 22 0.346837800997001 1108 23 0.4293322019479575 1108 24 0.3992761975520267 1108 25 0.20815143100436728 1109 1 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-2.1314607513611747 2315 20 -2.167645533950001 2315 21 -2.1333492545914154 2315 22 -2.2575689954308444 2315 23 -2.082397537665308 2315 24 -2.109148677047884 2315 25 -2.1554710293782406 2316 1 -1.5055060138549372 2316 2 -1.7355367131481252 2316 3 -1.178790214073235 2316 4 -1.5338979554168617 2316 5 -1.642432511529968 2316 6 -1.7307559887443098 2316 7 -1.7291732107079376 2316 8 -2.01777740057258 2316 9 -2.169828805491111 2316 10 -2.1712580917793325 2316 11 -2.3892432852223884 2316 12 -2.316621203275247 2316 13 -2.4718911037934177 2316 14 -2.7186781368604187 2316 15 -2.977502550273698 2316 16 -2.715401054206808 2316 17 -2.6937870061471756 2316 18 -2.5588214411752475 2316 19 -2.553043566141355 2316 20 -2.747169935819308 2316 21 -2.8790329115402145 2316 22 -2.6447172219067347 2316 23 -2.5982016297179698 2316 24 -2.4864684117339557 2316 25 -2.7001828240282677 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 144230.0 3 2.0 4 1222.0 5 5998.0 6 7058.0 7 6418.0 8 5906.0 9 5670.0 10 5478.0 11 5313.0 12 5310.0 13 5336.0 14 5362.0 15 5531.0 16 5611.0 17 6273.0 18 7210.0 19 8932.0 20 11511.0 21 14998.0 22 18435.0 23 21820.0 24 25567.0 25 28991.0 26 32321.0 27 36894.0 28 42526.0 29 50314.0 30 62463.0 31 78380.0 32 99013.0 33 126570.0 34 166953.0 35 223516.0 36 318573.0 37 437029.0 38 911947.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.66988512507807 19.111340073848407 13.22774860842312 24.99102619265041 2 16.026987775631486 21.722933279470627 39.30559783335372 22.944481111544164 3 18.129977406720794 25.118917804679015 30.249422602991633 26.501682185608562 4 12.30207958009713 17.463283798822353 38.69987954552632 31.534757075554193 5 13.251214647698681 37.76127193403971 35.898183877982035 13.089329540279577 6 30.900868379291342 37.533233650775756 18.658897177656936 12.907000792275971 7 27.33178908003957 32.04846297442745 22.883531357046824 17.736216588486155 8 24.916680080715217 36.558444160542045 21.09544214402002 17.42943361472272 9 26.16191702938281 15.687981142948932 20.671067596116526 37.479034231551736 10 15.031203719520041 29.029324398805844 34.57834651699115 21.361125364682966 11 33.463760590705746 22.895145518309114 25.08074728637839 18.56034660460675 12 22.999503171990447 24.926638912669997 31.943086534670478 20.130771380669078 13 28.499216044114796 22.23021780627511 26.143307203734462 23.12725894587563 14 21.084230151426137 22.201435135346237 27.97230249909158 28.74203221413605 15 22.856635404649943 30.509756404853363 23.91016208546868 22.723446105028014 16 22.221388326952905 28.249307819760443 27.599695858396885 21.929607994889768 17 21.68805381635566 28.52431214111138 27.919119252645704 21.868514789887257 18 21.50385729388005 27.71495452308756 29.644603269420355 21.136584913612037 19 23.222037595294832 27.186411277026306 27.84162745183937 21.74992367583949 20 23.29668075649723 27.459378397019435 28.266733499124015 20.977207347359318 21 22.968030482089734 26.880781612942663 27.576476900715868 22.574711004251736 22 23.058830401254063 27.74547079670781 27.660571582263266 21.53512721977486 23 22.350585973980106 27.587980996172746 27.882513150879383 22.17891987896776 24 22.817790480175066 27.0211052081791 28.383224504155972 21.777879807489857 25 22.745519803333536 27.24923344837692 28.071020256523543 21.934226491766 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 805.0 1 805.0 2 905.5 3 1006.0 4 1006.0 5 1006.0 6 2355.0 7 3704.0 8 3704.0 9 3704.0 10 5298.0 11 6892.0 12 6892.0 13 6892.0 14 11491.5 15 16091.0 16 16091.0 17 16091.0 18 27794.0 19 39497.0 20 39497.0 21 39497.0 22 60435.5 23 81374.0 24 81374.0 25 81374.0 26 113560.5 27 145747.0 28 145747.0 29 145747.0 30 184610.0 31 223473.0 32 223473.0 33 223473.0 34 252578.0 35 281683.0 36 281683.0 37 281683.0 38 304991.5 39 328300.0 40 328300.0 41 328300.0 42 346834.5 43 365369.0 44 365369.0 45 365369.0 46 372732.0 47 380095.0 48 380095.0 49 380095.0 50 367318.5 51 354542.0 52 354542.0 53 354542.0 54 317990.0 55 281438.0 56 281438.0 57 281438.0 58 240476.0 59 199514.0 60 199514.0 61 199514.0 62 161599.0 63 123684.0 64 123684.0 65 123684.0 66 94653.5 67 65623.0 68 65623.0 69 65623.0 70 47502.5 71 29382.0 72 29382.0 73 29382.0 74 20257.0 75 11132.0 76 11132.0 77 11132.0 78 7442.0 79 3752.0 80 3752.0 81 3752.0 82 2447.5 83 1143.0 84 1143.0 85 1143.0 86 728.5 87 314.0 88 314.0 89 314.0 90 201.0 91 88.0 92 88.0 93 88.0 94 58.5 95 29.0 96 29.0 97 29.0 98 16.5 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.502796737575309 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 2.3771675098253426E-4 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 3.735548944011253E-4 15 0.0 16 0.0 17 0.0 18 0.0 19 1.3583814341859102E-4 20 1.3583814341859102E-4 21 3.3959535854647755E-5 22 4.414739661104208E-4 23 3.735548944011253E-4 24 1.6979767927323877E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2944681.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.8052208099347 #Duplication Level Percentage of deduplicated Percentage of total 1 81.92269994335561 58.82477558778674 2 12.17151131920399 17.47956115732124 3 3.234600010066934 6.967835038640194 4 1.152397886752439 3.309927388766439 5 0.5448970623203706 1.9563226939299478 6 0.2891605249962897 1.2457941208125132 7 0.16585896979541256 0.8336677964627519 8 0.11107283983105407 0.6380487832044277 9 0.08039107729954692 0.5195249150978245 >10 0.2990825612223857 3.6091418292762434 >50 0.01529343795026658 0.7470532323467911 >100 0.010410571363664906 1.4489886583032257 >500 0.001621982884537925 0.7891998551044768 >1k 9.541075785478367E-4 1.3994860916200509 >5k 4.770537892739184E-5 0.2306728513271184 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6734 0.22868351444519797 No Hit TATCAACGCAGAGTACTTTTTTTTT 4236 0.14385259388028787 No Hit GGTATCAACGCAGAGTACTTTTTTT 4164 0.14140750729875323 No Hit GTCCTACAGTGGACATTTCTAAATT 3500 0.11885837549126714 No Hit CTGTAGGACGTGGAATATGGCAAGA 3471 0.11787354895148235 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3260 0.11070808688615166 No Hit CTTTAGGACGTGAAATATGGCGAGG 3141 0.1066669021194486 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.3959535854647755E-5 0.0 10 0.0 0.0 0.0 3.3959535854647755E-5 0.0 11 0.0 0.0 0.0 3.3959535854647755E-5 0.0 12 0.0 0.0 0.0 3.3959535854647755E-5 0.0 13 0.0 0.0 0.0 3.3959535854647755E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 695 0.0 11.971684 4 GGTATCA 1630 0.0 11.662231 1 GCACCGT 65 8.3539373E-4 11.636811 6 CGTTAGG 70 0.0010564764 11.315142 1 CGTTTAA 70 0.0010564764 11.315142 1 GGCGAGG 1005 0.0 11.101287 19 TAACGTG 60 0.006089599 11.030728 5 TATAACG 65 0.007837776 10.662345 2 GACCGTC 90 9.9911296E-5 10.505456 7 GTCCTAC 1800 0.0 10.395786 1 GTATAAG 375 0.0 10.296778 1 TTAGGAC 1545 0.0 10.158674 3 AACCGTC 75 0.0027553749 10.0852375 7 TGGCGAG 2125 0.0 9.966587 18 TTCAACG 110 4.3489224E-5 9.900748 2 GTATCAA 3215 0.0 9.854556 1 AGGACGT 2345 0.0 9.716986 5 AATCCCG 215 1.0913936E-11 9.674791 19 AGGACCT 1450 0.0 9.520116 5 TCCTACA 2060 0.0 9.5162525 2 >>END_MODULE