FastQCFastQC Report
Thu 26 May 2016
SRR1512088_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512088_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2944681
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT84180.2858713728244248No Hit
TATCAACGCAGAGTACTTTTTTTTT55340.18793207141962065No Hit
GGTATCAACGCAGAGTACTTTTTTT53600.18202311218091194No Hit
GTCCTACAGTGGACATTTCTAAATT38490.1307102535045392No Hit
GTCCTAAAGTGTGTATTTCTCATTT37670.12792557156445808No Hit
CTGTAGGACGTGGAATATGGCAAGA37460.1272124213115105No Hit
CTTTAGGACGTGAAATATGGCGAGG35290.11984320203105192No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGT1157.106064E-811.56238619
GGTATCA18650.011.5067811
TCCAACG2300.011.14925418
GGCGAGG10200.010.98750419
AGGACGT21650.010.445655
GGACGTG21350.010.3196066
TTAGGAC14600.09.9576073
TCAGGAC5650.09.7542973
GACGTGG10350.09.7260947
TAGGACC6650.09.7163614
GACGTGA12350.09.6889477
TAGGACG23650.09.64265254
TAAGACT2401.8189894E-129.5020294
GTCCTAA13800.09.3996121
TGTAGGA22300.09.3742012
GTATCAA35450.09.3360541
TGGCGAG22400.09.20071418
GTCCTAT2501.8189894E-129.1563291
GTAGGAC20650.09.0648913
TACATCG1054.4848977E-49.0495525
Produced by FastQC (version 0.11.5)