FastQCFastQC Report
Thu 26 May 2016
SRR1512087_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512087_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1689193
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT51540.3051161116580521No Hit
GGTATCAACGCAGAGTACTTTTTTT33260.19689875579640692No Hit
TATCAACGCAGAGTACTTTTTTTTT32430.19198516688146353No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11900.013.2674191
TAGGACC3950.011.0148764
GTAGGAC9250.010.5320843
CTAAGAC1356.064329E-810.5093613
TTAGTAC1002.5176316E-510.4042683
ACTGGTC2100.010.3595348
CTAGGAC2200.010.3182823
TAGGACG10300.010.2848894
GGACGTG9600.010.2466296
AGGACGT9850.010.1786115
GACAGTC1502.6160706E-810.0889877
GTCCTAA5000.010.0649961
TAGACAG2550.010.0148045
TTAGGAC6500.09.8949693
GACGTGA5850.09.8626327
GTATCAA22850.09.7596811
GTCCTAC8050.09.6837731
TGGCGAG9650.09.60544818
GGTCTAC1853.4833647E-99.6009491
CGTGAAA6900.09.5957889
Produced by FastQC (version 0.11.5)