##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512087_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1689193 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.63689110717366 31.0 30.0 34.0 25.0 34.0 2 27.07403239298292 31.0 26.0 34.0 10.0 34.0 3 28.774633804426138 31.0 28.0 34.0 16.0 34.0 4 33.14046470711162 35.0 33.0 37.0 27.0 37.0 5 33.5496186640603 35.0 35.0 37.0 28.0 37.0 6 33.81830317790803 37.0 35.0 37.0 29.0 37.0 7 33.88563651400403 37.0 35.0 37.0 30.0 37.0 8 33.93610972813645 37.0 35.0 37.0 30.0 37.0 9 35.55434814139059 39.0 35.0 39.0 30.0 39.0 10 35.43446545184594 39.0 35.0 39.0 30.0 39.0 11 35.45483198189905 39.0 35.0 39.0 30.0 39.0 12 35.371017994983404 39.0 35.0 39.0 30.0 39.0 13 35.3596622766019 39.0 35.0 39.0 30.0 39.0 14 36.50430412628989 40.0 37.0 41.0 30.0 41.0 15 36.43661855098855 40.0 36.0 41.0 30.0 41.0 16 36.405668268812384 40.0 36.0 41.0 30.0 41.0 17 36.352881523899285 40.0 36.0 41.0 30.0 41.0 18 36.30861778375828 40.0 36.0 41.0 30.0 41.0 19 36.33181643542212 40.0 37.0 41.0 29.0 41.0 20 36.266351447111134 40.0 36.0 41.0 29.0 41.0 21 36.203508420885 40.0 36.0 41.0 29.0 41.0 22 36.103946677496296 40.0 36.0 41.0 29.0 41.0 23 35.99815000417359 40.0 36.0 41.0 29.0 41.0 24 35.89883394023063 40.0 36.0 41.0 28.0 41.0 25 35.77589712957607 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5269799664413028 1101 2 2.928624593327374 1101 3 1.4556126074129807 1101 4 0.6099494554319307 1101 5 0.2412815471327434 1101 6 0.06333860588510021 1101 7 -0.06343087883633558 1101 8 -0.22767821782871778 1101 9 -0.08907845043140128 1101 10 -0.41010145237704165 1101 11 -0.10057243102093594 1101 12 -0.3227178405549438 1101 13 -0.16105685841142758 1101 14 -0.2397982255424438 1101 15 -0.16666284714066393 1101 16 -0.090629549485989 1101 17 -0.41350475272341924 1101 18 -0.31958003520884404 1101 19 -0.15127701192095344 1101 20 -0.3266299021900352 1101 21 -0.3100952011131426 1101 22 -0.2854957399524096 1101 23 -0.3017647336750784 1101 24 -0.4063909133609229 1101 25 -0.42838805093722954 1102 1 1.099716284849265 1102 2 3.712285237475058 1102 3 2.3389677614672735 1102 4 1.3566510352521277 1102 5 0.9445965510604779 1102 6 0.7575587333181133 1102 7 0.6432322965149808 1102 8 0.48656984012711746 1102 9 0.5654139995991514 1102 10 0.6463915213508074 1102 11 0.6380642103336953 1102 12 0.6507324518427211 1102 13 0.6227521666387617 1102 14 0.6202244680073505 1102 15 0.6005118059098677 1102 16 0.6630441870942647 1102 17 0.5248892422141793 1102 18 0.5369391233841512 1102 19 0.6848231888296752 1102 20 0.8783085633768692 1102 21 0.6775944209519693 1102 22 0.6768696261992844 1102 23 0.5772140565841468 1102 24 0.4472095055969163 1102 25 0.48200734052650773 1103 1 0.9272053387232404 1103 2 2.962295757779245 1103 3 1.8820111685763266 1103 4 1.2046029157147515 1103 5 0.8881415929914027 1103 6 0.7526238757095953 1103 7 0.6578050886705071 1103 8 0.42994889503642497 1103 9 0.5012428158812412 1103 10 0.583695184086551 1103 11 0.5139860728617052 1103 12 0.5796008344878913 1103 13 0.46857226609738234 1103 14 0.4344866607059288 1103 15 0.4422303226471769 1103 16 0.38042272767759044 1103 17 0.6137724534785818 1103 18 0.4813374788769238 1103 19 0.5491159405070789 1103 20 0.4993601930220848 1103 21 0.6222723159559891 1103 22 0.5337096607993956 1103 23 0.4646558191859711 1103 24 0.399057572365777 1103 25 0.4759276066401057 1104 1 1.2319435922481965 1104 2 3.849127342738214 1104 3 2.307643072934347 1104 4 1.5410014039274387 1104 5 1.1737727524334716 1104 6 0.8422777773048935 1104 7 0.6215440178472988 1104 8 0.5615761965980042 1104 9 0.7393016171938598 1104 10 0.7021250580912053 1104 11 0.5625366232500468 1104 12 0.6470075599027254 1104 13 0.6478983654691675 1104 14 0.8485743281649931 1104 15 0.46546222543695137 1104 16 0.6214347286655908 1104 17 0.5765037858195683 1104 18 0.5856551162649026 1104 19 0.7886116454785466 1104 20 0.6568364047193569 1104 21 0.67173375479382 1104 22 0.8517234273688743 1104 23 0.8830492968587507 1104 24 1.0391622134535154 1104 25 0.9374611925478646 1105 1 1.1422548783312543 1105 2 3.7033598364940445 1105 3 2.0565888582343455 1105 4 1.4582810393647492 1105 5 1.0172756759739627 1105 6 0.47083047757297436 1105 7 0.27134259513173475 1105 8 0.29542803478472024 1105 9 0.2725890269330691 1105 10 0.3624317648984956 1105 11 0.25607349884943886 1105 12 0.08668181639188077 1105 13 0.11044839405693097 1105 14 0.04619820738724911 1105 15 0.23110372309562877 1105 16 0.30067772204657217 1105 17 0.37701025371949726 1105 18 0.3553024989471325 1105 19 0.46607362767693417 1105 20 0.5248103237813098 1105 21 0.32932724070862207 1105 22 0.44008971186227086 1105 23 0.3896993865471359 1105 24 0.45085971172974126 1105 25 0.6277327174336094 1106 1 0.44163882147378786 1106 2 2.974004556845127 1106 3 1.5606006114026663 1106 4 0.7119699969147604 1106 5 0.4579778369060321 1106 6 0.17432802962731841 1106 7 0.12708229880346522 1106 8 -0.1513059551205842 1106 9 -0.23216865863181368 1106 10 -0.02168555474300149 1106 11 -0.0025757198147786653 1106 12 -0.08457214519070533 1106 13 -0.161854795277371 1106 14 0.03759158638527538 1106 15 -0.18613753870391747 1106 16 -0.2982832736406493 1106 17 -0.10047735509386513 1106 18 -0.1902959319443127 1106 19 -0.2972689067514409 1106 20 -0.22005429138194188 1106 21 -0.29780122248131846 1106 22 -0.5146696776090991 1106 23 -0.3351310747571503 1106 24 -0.3733160708366441 1106 25 -0.3881173993786433 1107 1 1.0480985271366983 1107 2 3.5501787862454037 1107 3 1.9327831879190285 1107 4 1.2282079372890067 1107 5 0.795036137611504 1107 6 0.6295961443777429 1107 7 0.41948142196216054 1107 8 0.2772447968945073 1107 9 0.3449857657338242 1107 10 0.29638822115147434 1107 11 0.3728661323697864 1107 12 0.3686591346143544 1107 13 0.23649881693643948 1107 14 0.4859670788610444 1107 15 0.5459179284396711 1107 16 0.4836829736769772 1107 17 0.43601817440168134 1107 18 0.32420959452061027 1107 19 0.5838397294287319 1107 20 0.5383675709409204 1107 21 0.45793249337154407 1107 22 0.5403595209889289 1107 23 0.5710539045109613 1107 24 0.6906374886470914 1107 25 0.6631031387127138 1108 1 0.7229096910213926 1108 2 2.508429421973105 1108 3 1.5668980433729196 1108 4 1.0862005175451728 1108 5 0.7335315106561282 1108 6 0.7404675099558915 1108 7 0.512585188040326 1108 8 0.6268080626721755 1108 9 0.6366695776856375 1108 10 0.4784977597280431 1108 11 0.5642824778733484 1108 12 0.5803528897498325 1108 13 0.5737178019269038 1108 14 0.5105654248656677 1108 15 0.4384565815926109 1108 16 0.5196728202066652 1108 17 0.4604760047886174 1108 18 0.5839188018307979 1108 19 0.7345983839576178 1108 20 0.7949747256197384 1108 21 0.7061333742389593 1108 22 0.7720950868222829 1108 23 0.8191165413757702 1108 24 0.8646191885161159 1108 25 0.7766997933376629 1109 1 0.910658191222641 1109 2 3.181510754736909 1109 3 1.5099298536690107 1109 4 1.0413127742018702 1109 5 0.6673798205709716 1109 6 0.34789922074649837 1109 7 0.327941531531458 1109 8 0.18914056473898455 1109 9 0.1532846915574737 1109 10 0.008335297881814085 1109 11 -0.0099958721907214 1109 12 0.11669558302092042 1109 13 0.247447152835214 1109 14 0.22426757068790693 1109 15 -0.015262825253486767 1109 16 -0.017087614490911562 1109 17 0.19700311403416748 1109 18 0.12927147711111076 1109 19 0.07232578627082376 1109 20 0.12844363031663875 1109 21 0.16314671115293322 1109 22 0.17057046034895507 1109 23 0.13362750984504856 1109 24 0.10429087229623946 1109 25 0.16877671743748124 1110 1 0.3422865659737617 1110 2 1.851844980205378 1110 3 1.0606373974045056 1110 4 0.6674440821263374 1110 5 0.5447233741422792 1110 6 0.43511004130696307 1110 7 0.4652109378975524 1110 8 0.28875099582761266 1110 9 0.21973856603532482 1110 10 0.23374746140173386 1110 11 0.1856117941526776 1110 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-1.3329267362158959 2315 14 -1.462519482708096 2315 15 -1.6071051446594637 2315 16 -1.7230695574646901 2315 17 -1.466678627683308 2315 18 -1.6805031361480758 2315 19 -1.5901142030715363 2315 20 -1.6553846178621114 2315 21 -1.5464749525592865 2315 22 -1.8004677711387274 2315 23 -1.8220856161887156 2315 24 -1.6630986587710055 2315 25 -1.7828713000027676 2316 1 -1.0556460339913158 2316 2 -1.4141831072999231 2316 3 -0.6540045243196602 2316 4 -1.182713317659136 2316 5 -1.0248313665596598 2316 6 -1.079339308888855 2316 7 -1.088280543556209 2316 8 -1.3506892496262495 2316 9 -1.4215984590837891 2316 10 -1.5704302432055144 2316 11 -1.7921925910901564 2316 12 -1.6125729130187096 2316 13 -1.8355798954004001 2316 14 -2.139724680225548 2316 15 -2.262920688369306 2316 16 -1.915903181326783 2316 17 -1.8617418282155214 2316 18 -1.8645508333918457 2316 19 -2.2816858373589852 2316 20 -2.3333008950518135 2316 21 -2.5717521338408105 2316 22 -2.1710073125243383 2316 23 -2.2462336932251574 2316 24 -1.9848118521452633 2316 25 -2.46691714459935 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 48834.0 3 0.0 4 379.0 5 1991.0 6 2350.0 7 2285.0 8 2076.0 9 1990.0 10 1864.0 11 1886.0 12 1904.0 13 1880.0 14 2007.0 15 2037.0 16 2139.0 17 2425.0 18 2654.0 19 3471.0 20 4423.0 21 5389.0 22 6743.0 23 8127.0 24 9621.0 25 11185.0 26 12934.0 27 15034.0 28 17635.0 29 21929.0 30 27854.0 31 36847.0 32 49148.0 33 67092.0 34 93472.0 35 133295.0 36 198993.0 37 282809.0 38 604491.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.66285735259381 18.95455403852609 13.178304669744664 25.204283939135436 2 16.19032678834353 21.568635927942527 38.993744873859896 23.247292409854047 3 18.064780045856217 24.963518082303207 30.368584288473844 26.603117583366732 4 12.56540845243853 17.223313144205544 38.16846269194817 32.042815711407755 5 13.394384182269285 37.56586725140348 35.57941573283811 13.460332833489128 6 31.148187329689385 37.08421713800614 18.457689559452355 13.309905972852127 7 27.367032659974317 32.031094137851625 22.71812634790696 17.883746854267095 8 25.135952734742688 35.8909891723252 21.015516312552172 17.957541780379945 9 26.117619478650454 15.633323131223015 20.63701424289587 37.612043147230665 10 15.433523581970798 28.46820937571965 34.10551665795442 21.992750384355134 11 33.338049589360125 22.95036742397109 24.921604576860073 18.789978409808707 12 22.994826523671364 24.806756835956577 31.54873362605694 20.64968301431512 13 28.20725636442964 22.040583876442774 26.281129509771826 23.471030249355756 14 21.391249986679973 21.755093873617234 27.867387013626683 28.98626912607611 15 23.04502801041681 29.72934413059964 24.13981114058607 23.085816718397485 16 22.534133162995584 27.95530173283929 27.142605966280943 22.367959137884185 17 21.834581267256773 28.162340337865675 27.76534579846459 22.23773259641296 18 21.632519197036693 27.35750148147666 29.26521717767005 21.744762143816605 19 23.14077719044724 26.989551778459102 27.996308283215537 21.873362747878122 20 23.279200751131018 26.94185246621746 28.17185318845882 21.607093594192705 21 22.87627627663183 26.64648343496976 27.778623021316122 22.698617267082287 22 23.20002794256855 27.129155643066838 27.72299442510237 21.947821989262238 23 22.531247354490308 27.098899290367 27.960084845750877 22.40976850939182 24 22.82620149586842 26.640405497085577 28.34649351817976 22.186899488866235 25 22.741286958498502 26.862192101312342 28.083249269473214 22.313271670715938 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1199.0 1 1199.0 2 1007.0 3 815.0 4 815.0 5 815.0 6 1678.5 7 2542.0 8 2542.0 9 2542.0 10 3532.0 11 4522.0 12 4522.0 13 4522.0 14 6940.5 15 9359.0 16 9359.0 17 9359.0 18 15853.0 19 22347.0 20 22347.0 21 22347.0 22 33403.0 23 44459.0 24 44459.0 25 44459.0 26 61886.0 27 79313.0 28 79313.0 29 79313.0 30 99834.0 31 120355.0 32 120355.0 33 120355.0 34 136994.5 35 153634.0 36 153634.0 37 153634.0 38 168443.5 39 183253.0 40 183253.0 41 183253.0 42 194939.0 43 206625.0 44 206625.0 45 206625.0 46 211413.5 47 216202.0 48 216202.0 49 216202.0 50 211615.0 51 207028.0 52 207028.0 53 207028.0 54 188057.5 55 169087.0 56 169087.0 57 169087.0 58 144714.5 59 120342.0 60 120342.0 61 120342.0 62 98470.5 63 76599.0 64 76599.0 65 76599.0 66 59118.0 67 41637.0 68 41637.0 69 41637.0 70 30314.0 71 18991.0 72 18991.0 73 18991.0 74 13153.0 75 7315.0 76 7315.0 77 7315.0 78 4877.5 79 2440.0 80 2440.0 81 2440.0 82 1618.5 83 797.0 84 797.0 85 797.0 86 519.5 87 242.0 88 242.0 89 242.0 90 153.5 91 65.0 92 65.0 93 65.0 94 42.5 95 20.0 96 20.0 97 20.0 98 12.5 99 5.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.1464178456813405 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 1.775995993353039E-4 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 4.1439906511570913E-4 15 0.0 16 0.0 17 5.91998664451013E-5 18 0.0 19 3.551991986706078E-4 20 4.1439906511570913E-4 21 1.183997328902026E-4 22 8.287981302314183E-4 23 5.91998664451013E-4 24 4.1439906511570913E-4 25 5.91998664451013E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1689193.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.93832410079867 #Duplication Level Percentage of deduplicated Percentage of total 1 81.21942057586229 58.42789000665457 2 12.50306210976313 17.98898668609111 3 3.4040737093786437 7.346500733048663 4 1.2692905959906404 3.6524255308988236 5 0.58616377506061 2.1083819813228906 6 0.3030388698793116 1.3080065061910606 7 0.18489973937564594 0.9310964164150894 8 0.11998729038483608 0.6905347666944786 9 0.08071802366453673 0.5226047412439843 >10 0.3072980007689279 3.574786595587598 >50 0.012102811523503022 0.6019347155904442 >100 0.008204503993281759 1.1916565666641712 >500 9.942824883186804E-4 0.47780046616135086 >1k 6.628549922124535E-4 0.8701857541778393 >5k 8.285687402655669E-5 0.3072085332579344 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5154 0.3051161116580521 No Hit GGTATCAACGCAGAGTACTTTTTTT 3326 0.19689875579640692 No Hit TATCAACGCAGAGTACTTTTTTTTT 3243 0.19198516688146353 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 5.91998664451013E-5 0.0 10 0.0 0.0 0.0 1.183997328902026E-4 0.0 11 0.0 0.0 0.0 1.183997328902026E-4 0.0 12 0.0 0.0 0.0 1.183997328902026E-4 0.0 13 0.0 0.0 0.0 1.183997328902026E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1190 0.0 13.267419 1 TAGGACC 395 0.0 11.014876 4 GTAGGAC 925 0.0 10.532084 3 CTAAGAC 135 6.064329E-8 10.509361 3 TTAGTAC 100 2.5176316E-5 10.404268 3 ACTGGTC 210 0.0 10.359534 8 CTAGGAC 220 0.0 10.318282 3 TAGGACG 1030 0.0 10.284889 4 GGACGTG 960 0.0 10.246629 6 AGGACGT 985 0.0 10.178611 5 GACAGTC 150 2.6160706E-8 10.088987 7 GTCCTAA 500 0.0 10.064996 1 TAGACAG 255 0.0 10.014804 5 TTAGGAC 650 0.0 9.894969 3 GACGTGA 585 0.0 9.862632 7 GTATCAA 2285 0.0 9.759681 1 GTCCTAC 805 0.0 9.683773 1 TGGCGAG 965 0.0 9.605448 18 GGTCTAC 185 3.4833647E-9 9.600949 1 CGTGAAA 690 0.0 9.595788 9 >>END_MODULE