FastQCFastQC Report
Thu 26 May 2016
SRR1512087_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512087_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1689193
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT65960.3904823190718882No Hit
TATCAACGCAGAGTACTTTTTTTTT47500.28119936561423114No Hit
GGTATCAACGCAGAGTACTTTTTTT45260.26793859553052846No Hit
ACGCAGAGTACTTTTTTTTTTTTTT20550.12165572554468317No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14550.013.6988881
CGAGTAA550.003076630412.08670910
TGCGCCA752.0807218E-411.3960412
AGTACCG700.001489211910.8591585
TAGGACC3500.010.3161994
GGCGAGG4050.09.84930419
TACACAG5800.09.66558555
GTATAAT1606.2484105E-89.5367851
TATAGTG2800.09.5017625
AACGTGT900.00111686099.4969816
AGGACGT9150.09.2421515
ATTATGC1651.06203515E-79.213833
GTATCAA28050.09.2137931
GGACGTG8900.09.1768576
ATTTAGA3950.09.1746281
TACTATA1252.7474167E-59.1216922
TTAGGAC6200.09.0423
TAGTACT2002.6448106E-99.0266744
ACCCCTA950.00182891098.997146
GTCCTAT1701.5870864E-78.9757981
Produced by FastQC (version 0.11.5)