FastQCFastQC Report
Thu 26 May 2016
SRR1512086_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512086_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2780830
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT69670.25053671026276325No Hit
TATCAACGCAGAGTACTTTTTTTTT43420.1561404328923379No Hit
GGTATCAACGCAGAGTACTTTTTTT43170.15524142072690528No Hit
GTCCTACAGTGGACATTTCTAAATT28220.10148049323403445No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGGG450.00883470713.1839772
CCGTAGG801.9030782E-512.3599791
GGTATCA16850.011.9712081
TAGGACC7000.010.9422984
AGGACGT18200.010.5993785
GGCGAGG8900.010.51900319
TGGCGAG16550.010.51354518
GGACGTG18650.010.1408126
TTAGGAC11800.010.0172753
TCTATAC2850.09.9540083
GTAGGAC19700.09.8883213
CGGTAGG800.00324508669.8879821
GTATCAA32050.09.8108521
AGGACCG1554.3675755E-89.761355
GACGTGA10300.09.6399257
TGTAGGA19050.09.6025032
GACGTGG9950.09.5988657
TAGGACG20300.09.5960564
CGTGGAA9800.09.5528019
TAGGACA6550.09.5284944
Produced by FastQC (version 0.11.5)