FastQCFastQC Report
Thu 26 May 2016
SRR1512086_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512086_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2780830
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT86870.3123887472445277No Hit
TATCAACGCAGAGTACTTTTTTTTT59600.21432450023913724No Hit
GGTATCAACGCAGAGTACTTTTTTT56250.20227773722234008No Hit
GTCCTACAGTGGACATTTCTAAATT30540.1098233261292492No Hit
GTCCTAAAGTGTGTATTTCTCATTT29030.10439329265003615No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC6650.012.8593094
GGTATCA19250.012.3372131
TAATACG600.005870266411.0852014
CGTGCGC909.554485E-510.5518310
TCTATAC3500.010.044553
TGGCGAG18650.09.77758818
GTGTTAA2850.09.7051351
AGGACCT14800.09.69420055
ATTTAGA7550.09.600971
TTTAGAA9250.09.5529612
GTCCTAA9300.09.5378051
GGCGAGA11250.09.4554619
GTCCTAC17450.09.3465021
GGCGAGG8350.09.32705419
GTATCAA37250.09.2433491
TAGAAAT8500.09.166254
AGGACGT16400.09.1539815
TTAGGAC10900.09.1529183
GTAGGAC19550.09.137093
GGACGTG15700.09.1340466
Produced by FastQC (version 0.11.5)