##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512086_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2780830 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.725517920908505 31.0 31.0 34.0 27.0 34.0 2 30.91907056526289 31.0 31.0 34.0 27.0 34.0 3 31.017191270232267 31.0 31.0 34.0 27.0 34.0 4 34.61755519035684 37.0 35.0 37.0 32.0 37.0 5 34.219183840795736 37.0 35.0 37.0 30.0 37.0 6 34.15480845646803 37.0 35.0 37.0 30.0 37.0 7 33.98194423966945 37.0 35.0 37.0 30.0 37.0 8 33.88046194841109 37.0 35.0 37.0 29.0 37.0 9 35.355315858934205 39.0 35.0 39.0 30.0 39.0 10 35.15912083802318 38.0 35.0 39.0 29.0 39.0 11 35.1451210609782 39.0 35.0 39.0 29.0 39.0 12 34.96445162055933 38.0 35.0 39.0 28.0 39.0 13 34.928974083277296 38.0 35.0 39.0 27.0 39.0 14 35.92237353595869 39.0 36.0 41.0 27.0 41.0 15 35.84331224850135 39.0 36.0 41.0 27.0 41.0 16 35.74064937446734 39.0 36.0 41.0 27.0 41.0 17 35.67093385787697 39.0 36.0 41.0 27.0 41.0 18 35.60175271411773 39.0 36.0 41.0 27.0 41.0 19 35.61979193262443 39.0 35.0 41.0 27.0 41.0 20 35.50007803425596 39.0 35.0 41.0 27.0 41.0 21 35.3785621559031 39.0 35.0 41.0 27.0 41.0 22 35.25192874069972 39.0 35.0 40.0 27.0 41.0 23 35.31776268236462 39.0 35.0 41.0 27.0 41.0 24 35.19774491788423 39.0 35.0 41.0 26.0 41.0 25 34.75042667117371 39.0 34.0 40.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.025151347726460216 1101 2 -0.05401599191387163 1101 3 -0.07629548577000378 1101 4 -0.10760738632511391 1101 5 -0.04210918162186772 1101 6 -0.09442662707089511 1101 7 -0.14590048184950177 1101 8 -0.18003221063101904 1101 9 -0.19123235531337457 1101 10 -0.12346190840673898 1101 11 -0.25648755559242886 1101 12 -0.46840498292188215 1101 13 -0.3518263650111919 1101 14 -0.31185881387025916 1101 15 -0.21706910093381993 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0.1516593449133694 1103 3 0.17165823030072147 1103 4 0.2537946449098527 1103 5 0.09610706894033427 1103 6 0.14603686741489952 1103 7 0.1674566522814871 1103 8 0.2691566808437642 1103 9 0.48325913639058626 1103 10 0.18163255882917184 1103 11 0.19842035224647248 1103 12 0.3112882638804706 1103 13 0.2385851576506397 1103 14 0.4468910504843038 1103 15 0.3333465712840109 1103 16 0.5232043354753984 1103 17 0.5774518818224408 1103 18 0.2858621336674929 1103 19 0.28926114270093706 1103 20 0.46465763437727503 1103 21 0.5748286544314851 1103 22 0.3982534238230713 1103 23 0.2627341070240945 1103 24 0.43170507191973684 1103 25 0.1908412080444748 1104 1 0.18717459923363577 1104 2 0.13179338218818515 1104 3 0.1308204481752 1104 4 0.09805696470508707 1104 5 0.22239194239145377 1104 6 0.1804843814986441 1104 7 0.20842840613791935 1104 8 0.19578172713413977 1104 9 0.19337288782845263 1104 10 0.059985178454986965 1104 11 0.2988946934483536 1104 12 0.33695512390163884 1104 13 0.2689224137863704 1104 14 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2314 24 -0.346081546238203 2314 25 -0.6648349575976766 2315 1 -0.3676700243407822 2315 2 -0.38647365423127766 2315 3 -0.5098838655083284 2315 4 -0.4942514638158073 2315 5 -0.4379864300423506 2315 6 -0.556106343175216 2315 7 -0.5747654376563816 2315 8 -0.7262443041759568 2315 9 -0.6689435361507847 2315 10 -0.6285996254185022 2315 11 -0.5747924017629913 2315 12 -0.697396337424415 2315 13 -0.7971174565029244 2315 14 -0.9111422657307386 2315 15 -0.9492495774729974 2315 16 -1.0766025106960697 2315 17 -0.982789393640914 2315 18 -0.9246194919354878 2315 19 -0.7384936486697669 2315 20 -0.9826515398054951 2315 21 -0.8386166714774959 2315 22 -0.9606816929807493 2315 23 -1.0058595293169574 2315 24 -0.7943122711193737 2315 25 -0.88934667467921 2316 1 -0.9140708034724412 2316 2 -0.8443583875590903 2316 3 -1.0087989465003773 2316 4 -0.8880627505157506 2316 5 -1.0274231536034222 2316 6 -1.135530734741188 2316 7 -1.3001804274147304 2316 8 -1.37434244197879 2316 9 -1.5674902224728555 2316 10 -1.7120943167705818 2316 11 -1.6644744059881589 2316 12 -1.4824236931176245 2316 13 -1.54676034762673 2316 14 -1.6716991166812392 2316 15 -1.7755341294043347 2316 16 -1.8797611737166804 2316 17 -1.7242372127024552 2316 18 -1.6647041601022536 2316 19 -1.504292318346529 2316 20 -1.687094517920528 2316 21 -1.721852263246447 2316 22 -1.6618833005061475 2316 23 -1.7552507554772632 2316 24 -1.6572879065734085 2316 25 -1.531363870274781 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 46147.0 3 1.0 4 845.0 5 7457.0 6 9416.0 7 7429.0 8 6156.0 9 5644.0 10 5235.0 11 4997.0 12 4813.0 13 4463.0 14 4459.0 15 4563.0 16 4657.0 17 5001.0 18 5167.0 19 6030.0 20 7241.0 21 9439.0 22 11972.0 23 15208.0 24 18750.0 25 22170.0 26 25604.0 27 29336.0 28 34832.0 29 42306.0 30 53847.0 31 68459.0 32 88463.0 33 114706.0 34 149348.0 35 198275.0 36 284347.0 37 490723.0 38 983324.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.766490041496624 19.848718429289082 13.655317659880808 24.72947386933349 2 15.149253999705122 21.404257002405757 39.88384762822611 23.562641369663016 3 18.234591830496647 25.680210584609632 30.69367778684781 25.39151979804591 4 12.822502634105645 18.060147509916106 38.2504863655815 30.86686349039675 5 13.110487771354883 38.03005367550176 35.88979791270733 12.969660640436024 6 30.959030217596904 37.888508107291706 18.375161372683696 12.777300302427694 7 27.35812689017308 32.12490515421655 22.83908041843622 17.677887537174154 8 25.196243009045087 36.48023400998872 20.91129830375843 17.41222467720776 9 26.398125739437507 15.390872509286796 20.404987000284088 37.806014750991615 10 15.091069932358325 29.0422283994347 34.46025107611756 21.406450592089413 11 33.78009443223785 22.769281113912033 24.89940053868809 18.551223915162023 12 22.983785416584258 25.06510646102063 31.86732018857679 20.08378793381832 13 28.646015757885234 22.10300521786661 26.200379023528946 23.05060000071921 14 21.24768504367401 21.93568107363629 27.957480320623702 28.859153562066002 15 23.032943401790114 30.29448042490911 23.858200609170645 22.814375564130135 16 22.302945523458824 28.1407709209121 27.38117756209477 22.175105993534306 17 21.762099804734557 28.375772700956187 27.82442652013967 22.03770097416958 18 21.54270487588238 27.62506158233333 29.494071913781138 21.33816162800315 19 23.292819006719945 27.000061492498457 27.92485832452001 21.782261176261596 20 23.389995073413335 27.233919369396908 28.214238195071257 21.161847362118504 21 23.148987892104156 26.733709000550192 27.576155320533797 22.54114778681185 22 23.162113469719472 27.45284681192306 27.74279621551839 21.642243502839083 23 22.56103393591122 27.392505115379223 27.914579460089257 22.1318814886203 24 22.828111008418777 26.88695971143853 28.391406449478367 21.89352283066433 25 22.82151170547394 27.117660322073593 28.09461186071743 21.966216111735033 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 939.0 1 939.0 2 972.0 3 1005.0 4 1005.0 5 1005.0 6 2412.5 7 3820.0 8 3820.0 9 3820.0 10 5539.0 11 7258.0 12 7258.0 13 7258.0 14 11303.5 15 15349.0 16 15349.0 17 15349.0 18 26839.5 19 38330.0 20 38330.0 21 38330.0 22 57564.5 23 76799.0 24 76799.0 25 76799.0 26 106978.0 27 137157.0 28 137157.0 29 137157.0 30 172912.0 31 208667.0 32 208667.0 33 208667.0 34 234950.0 35 261233.0 36 261233.0 37 261233.0 38 284466.5 39 307700.0 40 307700.0 41 307700.0 42 325316.5 43 342933.0 44 342933.0 45 342933.0 46 348834.5 47 354736.0 48 354736.0 49 354736.0 50 345080.0 51 335424.0 52 335424.0 53 335424.0 54 302333.5 55 269243.0 56 269243.0 57 269243.0 58 230173.5 59 191104.0 60 191104.0 61 191104.0 62 155275.0 63 119446.0 64 119446.0 65 119446.0 66 92077.5 67 64709.0 68 64709.0 69 64709.0 70 46699.5 71 28690.0 72 28690.0 73 28690.0 74 19927.5 75 11165.0 76 11165.0 77 11165.0 78 7407.0 79 3649.0 80 3649.0 81 3649.0 82 2403.5 83 1158.0 84 1158.0 85 1158.0 86 705.5 87 253.0 88 253.0 89 253.0 90 155.5 91 58.0 92 58.0 93 58.0 94 31.0 95 4.0 96 4.0 97 4.0 98 2.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.420342128069677 2 0.0 3 0.0 4 0.0 5 0.05523530744418033 6 0.0 7 0.0 8 0.003380285742026661 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 1.078814598519147E-4 20 0.0 21 0.0 22 0.0 23 0.0 24 0.005034468126422687 25 0.0013305380048402814 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2780830.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.03088656096136 #Duplication Level Percentage of deduplicated Percentage of total 1 81.26286925256431 56.90910778244568 2 12.470457750195319 17.466344241343798 3 3.3297980291772937 6.995661241566835 4 1.2318920386522905 3.4508196645683995 5 0.5525203333174912 1.9346744392590893 6 0.3197355744104532 1.3434819444625363 7 0.19305526594234684 0.9463882000443304 8 0.12894847143463756 0.7224300620198777 9 0.0900249956529579 0.5674077232401001 >10 0.38761838114013264 4.509828222251101 >50 0.018680777312837474 0.9087311176249646 >100 0.011785868803471065 1.5982408959623204 >500 0.0014859721666203225 0.7364497963985411 >1k 9.735679711569353E-4 1.1829917865988753 >5k 1.5372125860372665E-4 0.7274428822134983 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 8687 0.3123887472445277 No Hit TATCAACGCAGAGTACTTTTTTTTT 5960 0.21432450023913724 No Hit GGTATCAACGCAGAGTACTTTTTTT 5625 0.20227773722234008 No Hit GTCCTACAGTGGACATTTCTAAATT 3054 0.1098233261292492 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2903 0.10439329265003615 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 3.5960486617304904E-5 0.0 8 0.0 0.0 0.0 3.5960486617304904E-5 0.0 9 0.0 0.0 0.0 3.5960486617304904E-5 0.0 10 0.0 0.0 0.0 3.5960486617304904E-5 0.0 11 0.0 0.0 0.0 3.5960486617304904E-5 0.0 12 0.0 0.0 0.0 3.5960486617304904E-5 7.192097323460981E-5 13 0.0 0.0 0.0 3.5960486617304904E-5 1.0788145985191472E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 665 0.0 12.859309 4 GGTATCA 1925 0.0 12.337213 1 TAATACG 60 0.0058702664 11.085201 4 CGTGCGC 90 9.554485E-5 10.55183 10 TCTATAC 350 0.0 10.04455 3 TGGCGAG 1865 0.0 9.777588 18 GTGTTAA 285 0.0 9.705135 1 AGGACCT 1480 0.0 9.6942005 5 ATTTAGA 755 0.0 9.60097 1 TTTAGAA 925 0.0 9.552961 2 GTCCTAA 930 0.0 9.537805 1 GGCGAGA 1125 0.0 9.45546 19 GTCCTAC 1745 0.0 9.346502 1 GGCGAGG 835 0.0 9.327054 19 GTATCAA 3725 0.0 9.243349 1 TAGAAAT 850 0.0 9.16625 4 AGGACGT 1640 0.0 9.153981 5 TTAGGAC 1090 0.0 9.152918 3 GTAGGAC 1955 0.0 9.13709 3 GGACGTG 1570 0.0 9.134046 6 >>END_MODULE