FastQCFastQC Report
Thu 26 May 2016
SRR1512085_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512085_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2221170
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT51380.23131952979735904No Hit
GGTATCAACGCAGAGTACTTTTTTT31360.1411868519744099No Hit
TATCAACGCAGAGTACTTTTTTTTT30630.1379002957900566No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG552.0402405E-413.7535795
GGTATCA12700.011.8415171
CTGGTCG658.347512E-411.6376449
ACCGGGT752.1668416E-411.3467038
GTCCTAC12700.011.2961851
ACCGTGC1053.6679994E-610.8063848
GTATCAA24200.010.548041
TCCTACA15350.010.18394852
GGACGTG10800.010.0684486
AGGACGT11350.09.9970965
GGACTCG951.7241518E-49.9532496
TGGACCG1054.32327E-59.9058525
CTGTCCG1151.0855601E-59.8666989
GACGTGG5950.09.852887
GTAGGAC12050.09.651763
GCCTCGA1401.1283391E-69.455816
TAGGACC4300.09.4555864
TACACCG800.0046947029.4555865
CTACACT3000.09.45558554
GGACCGC900.00116267549.45558556
Produced by FastQC (version 0.11.5)