FastQCFastQC Report
Thu 26 May 2016
SRR1512085_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512085_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2221170
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT65310.2940342252056349No Hit
GGTATCAACGCAGAGTACTTTTTTT42390.1908453652804603No Hit
TATCAACGCAGAGTACTTTTTTTTT41660.187558809096107No Hit
GTCCTACAGTGGACATTTCTAAATT22380.10075770877510501No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC4900.012.2156464
TACCGAT550.003075920712.0873577
GGTATCA14600.012.0185961
CCAACGA1158.8519664E-710.73621719
TATACCG803.7681655E-410.6886915
GTCCTAC12600.010.5961061
GTAGGAC12750.010.3580173
GGCGAGG6550.010.29491619
GGACGTG12650.010.1353616
GTCCTAA6500.010.1233561
GTATCAA28850.010.1149651
GTGCAAC951.5821203E-410.0384161
TGGCGAG13750.09.94639118
TCCAACG1356.4691085E-79.84917818
GTATAAA3200.09.8345111
TGTAGGA14150.09.8032122
TCAATAC2054.1836756E-119.7327913
AGGACGT13450.09.6070185
TTAGGAC9900.09.5970293
ACTAGAC1304.2337797E-69.5010593
Produced by FastQC (version 0.11.5)