##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512085_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2221170 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.900855405034285 31.0 31.0 34.0 28.0 34.0 2 31.090434770864004 31.0 31.0 34.0 27.0 34.0 3 31.1908278069666 31.0 31.0 34.0 27.0 34.0 4 34.779346470553804 37.0 35.0 37.0 32.0 37.0 5 34.39657387773111 37.0 35.0 37.0 30.0 37.0 6 34.33475510654295 37.0 35.0 37.0 30.0 37.0 7 34.16852424623059 37.0 35.0 37.0 30.0 37.0 8 34.07960264185092 37.0 35.0 37.0 30.0 37.0 9 35.589234952750125 39.0 35.0 39.0 30.0 39.0 10 35.40145508898463 39.0 35.0 39.0 30.0 39.0 11 35.38621447255275 39.0 35.0 39.0 30.0 39.0 12 35.21446129742433 39.0 35.0 39.0 29.0 39.0 13 35.18256189305636 39.0 35.0 39.0 29.0 39.0 14 36.21199683049924 39.0 36.0 41.0 29.0 41.0 15 36.14242853991365 39.0 36.0 41.0 28.0 41.0 16 36.038105142785106 39.0 36.0 41.0 27.0 41.0 17 35.976628083397486 39.0 36.0 41.0 27.0 41.0 18 35.90833749780521 39.0 36.0 41.0 27.0 41.0 19 35.92363439088408 39.0 36.0 41.0 27.0 41.0 20 35.81420602655357 39.0 36.0 41.0 27.0 41.0 21 35.697227137049396 39.0 36.0 41.0 27.0 41.0 22 35.58118243988529 39.0 36.0 41.0 27.0 41.0 23 35.64109185699429 39.0 36.0 41.0 27.0 41.0 24 35.52767100221955 39.0 36.0 41.0 27.0 41.0 25 35.10207008018297 39.0 35.0 40.0 26.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1306110408674499 1101 2 0.08761149649020794 1101 3 0.011927462006070755 1101 4 -0.0044877648446757235 1101 5 0.04865901947300699 1101 6 -0.04480243821192431 1101 7 0.09914795658139042 1101 8 -0.038509399934341104 1101 9 0.030686030883465776 1101 10 0.21570599788925193 1101 11 0.12296025760250728 1101 12 -0.13634654911495403 1101 13 0.09580354303270155 1101 14 -0.040349297983958365 1101 15 0.14544139867025763 1101 16 -0.1970804180992971 1101 17 -0.17878346238207854 1101 18 -0.03177586222170703 1101 19 -0.2558630730480047 1101 20 -0.04165000376880812 1101 21 -0.17040986318918527 1101 22 -0.27389328353266507 1101 23 -0.14438203254848503 1101 24 -0.29966576027701564 1101 25 -0.18985333271798766 1102 1 0.10667193739774561 1102 2 0.27486916334964206 1102 3 0.20790824171473687 1102 4 0.24073510902722717 1102 5 0.18318609520901674 1102 6 0.12402866774701948 1102 7 0.2830018468067337 1102 8 0.2765624120948047 1102 9 0.30988043520494557 1102 10 0.39187432419409873 1102 11 0.27601775915828597 1102 12 0.42483781023654643 1102 13 0.4496755039935749 1102 14 0.4726371177169 1102 15 0.46429591824659155 1102 16 0.3321342310793298 1102 17 0.46171782668719885 1102 18 0.40679045860998997 1102 19 0.38473261867035546 1102 20 0.5663637909008088 1102 21 0.5991622925624256 1102 22 0.6293578644827633 1102 23 0.6271389107562371 1102 24 0.7256156652425929 1102 25 0.519674777266161 1103 1 -0.028306149841650807 1103 2 0.10410295301056749 1103 3 0.09306140513423244 1103 4 0.05085693862141483 1103 5 0.09853833091300856 1103 6 0.05010217547881979 1103 7 0.16417950499443634 1103 8 0.17353457728304988 1103 9 0.2918894228672002 1103 10 0.12093107146692716 1103 11 0.06658334934699184 1103 12 0.09310463553854476 1103 13 0.04833270943469614 1103 14 0.26783793867149086 1103 15 0.1961279471891757 1103 16 0.22324395887247306 1103 17 0.09747423945366052 1103 18 0.3472126829518132 1103 19 0.23815013917329964 1103 20 0.28669433974894787 1103 21 0.3998885763289124 1103 22 0.3822925345028594 1103 23 0.2501478114033233 1103 24 0.4896136019792223 1103 25 0.31290186006610554 1104 1 0.30912389868447576 1104 2 0.22865313219539019 1104 3 0.22541963103102347 1104 4 0.2967955399389126 1104 5 0.3259357643320939 1104 6 0.36929279541368487 1104 7 0.3633107188414826 1104 8 0.4339244467730552 1104 9 0.6232035192354246 1104 10 0.3423228393742548 1104 11 0.5204321054623193 1104 12 0.688901599062163 1104 13 0.5076741122808386 1104 14 0.638255466565333 1104 15 0.7098536779311431 1104 16 0.672581495490121 1104 17 0.7995819535708435 1104 18 0.8377602499540515 1104 19 0.8775437856711434 1104 20 0.6934135226859937 1104 21 0.7877041324462226 1104 22 0.8165963903222817 1104 23 0.8660888167218701 1104 24 0.7302163123045347 1104 25 0.9305523083999105 1105 1 -0.003009615072855354 1105 2 0.04236535592114521 1105 3 -0.10295204240154376 1105 4 0.06720600453070347 1105 5 -0.07009490774728988 1105 6 0.029193562215674262 1105 7 -0.11571499157800247 1105 8 -0.2137977002725009 1105 9 -0.1864491645715205 1105 10 -0.12677955124369333 1105 11 -0.11466036183583128 1105 12 -0.20058870314802846 1105 13 -0.06652525623517391 1105 14 0.19621667540611298 1105 15 0.04995291112916078 1105 16 -0.043250710193234454 1105 17 0.08961352777021148 1105 18 -0.14329523042484027 1105 19 -0.04397177589854806 1105 20 -0.09007768164418195 1105 21 -0.08599907007970842 1105 22 0.09490040475619566 1105 23 0.0686147526713512 1105 24 0.07708144536722727 1105 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11 0.20787384188582791 1107 12 0.34144309189732525 1107 13 0.16142248458810826 1107 14 0.27686373023152555 1107 15 0.26758666596340674 1107 16 0.335086969301436 1107 17 0.3314121788158104 1107 18 0.3091962730838773 1107 19 0.45473474031380334 1107 20 0.4772836333541477 1107 21 0.32411332418379146 1107 22 0.1300834826915107 1107 23 0.2526619786324744 1107 24 0.29421570557602905 1107 25 0.26230065320109475 1108 1 0.04369633397590178 1108 2 0.04063545589580997 1108 3 0.037133172408054804 1108 4 -0.06191841387995822 1108 5 -0.08350950139166713 1108 6 -0.09549594785902826 1108 7 0.0037788326074661427 1108 8 -0.0609478352815529 1108 9 -0.05972756744841945 1108 10 -0.059856035261958596 1108 11 0.11261864966311208 1108 12 0.008850847089554748 1108 13 -0.04344936693642154 1108 14 -0.015088324562995581 1108 15 0.09287389530499013 1108 16 0.10174074747200734 1108 17 0.07932700426875527 1108 18 -0.07751384622677193 1108 19 -0.05054033701717486 1108 20 -0.07693090486944953 1108 21 0.0017674337728337264 1108 22 0.11290495860843919 1108 23 -0.06573450833173666 1108 24 -0.1935632383037813 1108 25 -0.0775827979699173 1109 1 -0.24969095972203448 1109 2 -0.16984839063104218 1109 3 -0.2623917700092093 1109 4 -0.1289503484867538 1109 5 -0.14094268954146116 1109 6 -0.1716473139697854 1109 7 -0.11257581932725458 1109 8 -0.12045793462981891 1109 9 -0.21950883963725687 1109 10 -0.3016636641809072 1109 11 -0.1716456045899264 1109 12 -0.08827077371297065 1109 13 -0.20375812008975913 1109 14 -0.17500056500772132 1109 15 -0.3427914667302403 1109 16 -0.3106631369815247 1109 17 -0.23474479519900626 1109 18 -0.16559162188789145 1109 19 -0.21222912757764334 1109 20 -0.06112487643309805 1109 21 -0.06107044989560251 1109 22 -0.20412841917472946 1109 23 -0.16543292054908676 1109 24 -0.14114833519864334 1109 25 -0.0599343744957892 1110 1 -0.35864269120480685 1110 2 -0.374838838193579 1110 3 -0.3668329991518071 1110 4 -0.35279944935055596 1110 5 -0.3480359017828931 1110 6 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-0.3796910497184669 2315 6 -0.4696594852800189 2315 7 -0.23760352895452286 2315 8 -0.5136932298983297 2315 9 -0.621552521408411 2315 10 -0.499273159102664 2315 11 -0.46886974217190414 2315 12 -0.4715277109872176 2315 13 -0.5304288936506865 2315 14 -0.7963136108958864 2315 15 -0.916930690445561 2315 16 -0.8139206663018399 2315 17 -0.9115318768899598 2315 18 -0.8594231653877102 2315 19 -0.8725780842921012 2315 20 -0.6588402931595212 2315 21 -0.5699476532469276 2315 22 -0.7296154068358405 2315 23 -0.6244246286717399 2315 24 -0.6290545183542164 2315 25 -0.697239979657887 2316 1 -1.0427993637568278 2316 2 -1.0496717405040101 2316 3 -1.2219839060093456 2316 4 -0.9777054912415863 2316 5 -1.2195490730125442 2316 6 -1.3089642879229046 2316 7 -1.3999849913954918 2316 8 -1.3829217313408861 2316 9 -1.5958072253785787 2316 10 -1.8608835974864704 2316 11 -1.705845137387854 2316 12 -1.8448243911188058 2316 13 -1.8461039714181666 2316 14 -1.9269581611824123 2316 15 -2.0007050367825343 2316 16 -2.015962884380613 2316 17 -2.143427007661458 2316 18 -1.9422768256128435 2316 19 -1.8169420711212183 2316 20 -1.978066188739902 2316 21 -2.101431057794194 2316 22 -2.2247603355557857 2316 23 -2.1185631500822026 2316 24 -2.0634422535332746 2316 25 -2.1623003462054626 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 32438.0 3 2.0 4 573.0 5 5347.0 6 6844.0 7 5424.0 8 4242.0 9 3876.0 10 3857.0 11 3581.0 12 3524.0 13 3318.0 14 3330.0 15 3426.0 16 3412.0 17 3589.0 18 3892.0 19 4453.0 20 5413.0 21 7089.0 22 8956.0 23 11488.0 24 14064.0 25 16624.0 26 19763.0 27 22571.0 28 25931.0 29 32445.0 30 41122.0 31 52716.0 32 68014.0 33 88065.0 34 116367.0 35 152863.0 36 221043.0 37 381223.0 38 840285.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.97487884088742 19.55364978404086 13.478581094431089 24.99289028064063 2 15.178126843060188 21.11580833524674 39.71875182899103 23.987312992702044 3 18.299319727891156 25.231702210996907 30.483258823052715 25.985719238059218 4 12.918867083564066 17.708144806565908 37.85324851317098 31.519739596699036 5 13.37918123344275 37.67383845304554 35.759965656868694 13.187014656643012 6 31.36437102968255 37.41829756389651 18.213689181827593 13.003642224593346 7 27.729439889787816 31.864287740245008 22.54604555256914 17.860226817398036 8 25.29541854750334 36.26293623929915 20.812746557314753 17.62889865588275 9 26.416798354020628 15.51236510487716 20.156584142591516 37.9142523985107 10 15.266683774767353 28.80680001980938 34.09477887779864 21.83173732762463 11 33.949585128558375 22.761652642526236 24.577767572945792 18.7109946559696 12 23.125469910002387 24.776131498264427 31.57790713903033 20.520491452702856 13 28.69289608629686 21.883196693634435 26.06612731128189 23.35777990878681 14 21.36063426032226 21.741424564531307 27.690586492704295 29.207354682442137 15 23.124974675508852 29.965333585452715 23.822084757132505 23.087606981905935 16 22.611281441762674 28.028741609151936 27.006397529230092 22.3535794198553 17 21.9761657144658 28.22431421277975 27.578888603753875 22.22063146900057 18 21.90305109469334 27.43144378863392 29.054912501069257 21.610592615603487 19 23.390878210308554 26.956754676037125 27.73882174444492 21.9135453692094 20 23.599049149772416 27.073389249809786 27.880801559538444 21.446760040879358 21 23.218393909516156 26.605527717374176 27.407852618214722 22.768225754894942 22 23.368630046326935 27.23560105710054 27.53008549548211 21.865683401090415 23 22.72595073767429 27.113053030609997 27.670371921104643 22.490624310611075 24 22.970837400453117 26.668074400377478 28.152723199331493 22.208364999837915 25 22.92524427760327 26.93102328938145 27.90102024139845 22.24271219161683 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 932.0 1 932.0 2 850.0 3 768.0 4 768.0 5 768.0 6 1814.0 7 2860.0 8 2860.0 9 2860.0 10 3904.5 11 4949.0 12 4949.0 13 4949.0 14 7927.0 15 10905.0 16 10905.0 17 10905.0 18 19075.0 19 27245.0 20 27245.0 21 27245.0 22 42146.0 23 57047.0 24 57047.0 25 57047.0 26 80280.5 27 103514.0 28 103514.0 29 103514.0 30 131113.0 31 158712.0 32 158712.0 33 158712.0 34 181167.0 35 203622.0 36 203622.0 37 203622.0 38 223164.0 39 242706.0 40 242706.0 41 242706.0 42 257860.0 43 273014.0 44 273014.0 45 273014.0 46 279276.5 47 285539.0 48 285539.0 49 285539.0 50 279546.0 51 273553.0 52 273553.0 53 273553.0 54 246970.5 55 220388.0 56 220388.0 57 220388.0 58 189698.5 59 159009.0 60 159009.0 61 159009.0 62 130311.0 63 101613.0 64 101613.0 65 101613.0 66 78264.5 67 54916.0 68 54916.0 69 54916.0 70 40139.0 71 25362.0 72 25362.0 73 25362.0 74 17588.0 75 9814.0 76 9814.0 77 9814.0 78 6611.5 79 3409.0 80 3409.0 81 3409.0 82 2184.5 83 960.0 84 960.0 85 960.0 86 616.5 87 273.0 88 273.0 89 273.0 90 162.0 91 51.0 92 51.0 93 51.0 94 29.5 95 8.0 96 8.0 97 8.0 98 4.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.42360557724082354 2 0.0 3 0.0 4 0.0 5 0.05497102878212834 6 0.0 7 0.0 8 0.0037817906778859793 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 2.251065879694035E-4 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0047722596649513544 25 0.0013056182102225405 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2221170.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.42046745211101 #Duplication Level Percentage of deduplicated Percentage of total 1 82.07305527704646 58.61695973109605 2 11.981573373314419 17.11459142267765 3 3.1487120596457703 6.746474615160006 4 1.158336989670497 3.3091587707735197 5 0.5389000020821458 1.9244245029325227 6 0.2967054208203717 1.2714503910339756 7 0.19597260423840687 0.9797518501760201 8 0.1216898324029639 0.6952915771510981 9 0.08551381346997305 0.5496692878474293 >10 0.3713779193675955 4.410790635098706 >50 0.015541229727525016 0.7484958873892262 >100 0.010288984954221547 1.4603668098464815 >500 0.001512968600867453 0.744061974095217 >1k 7.564843004331124E-4 1.1344626383435448 >5k 6.304035836942603E-5 0.29404990637860423 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6531 0.2940342252056349 No Hit GGTATCAACGCAGAGTACTTTTTTT 4239 0.1908453652804603 No Hit TATCAACGCAGAGTACTTTTTTTTT 4166 0.187558809096107 No Hit GTCCTACAGTGGACATTTCTAAATT 2238 0.10075770877510501 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 4.50213175938807E-5 0.0 0.0 0.0 4.50213175938807E-5 7 4.50213175938807E-5 0.0 0.0 0.0 4.50213175938807E-5 8 4.50213175938807E-5 0.0 0.0 0.0 4.50213175938807E-5 9 4.50213175938807E-5 0.0 0.0 0.0 4.50213175938807E-5 10 4.50213175938807E-5 0.0 0.0 0.0 9.00426351877614E-5 11 4.50213175938807E-5 0.0 0.0 0.0 9.00426351877614E-5 12 4.50213175938807E-5 0.0 0.0 0.0 2.7012790556328424E-4 13 4.50213175938807E-5 0.0 0.0 0.0 3.151492231571649E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 490 0.0 12.215646 4 TACCGAT 55 0.0030759207 12.087357 7 GGTATCA 1460 0.0 12.018596 1 CCAACGA 115 8.8519664E-7 10.736217 19 TATACCG 80 3.7681655E-4 10.688691 5 GTCCTAC 1260 0.0 10.596106 1 GTAGGAC 1275 0.0 10.358017 3 GGCGAGG 655 0.0 10.294916 19 GGACGTG 1265 0.0 10.135361 6 GTCCTAA 650 0.0 10.123356 1 GTATCAA 2885 0.0 10.114965 1 GTGCAAC 95 1.5821203E-4 10.038416 1 TGGCGAG 1375 0.0 9.946391 18 TCCAACG 135 6.4691085E-7 9.849178 18 GTATAAA 320 0.0 9.834511 1 TGTAGGA 1415 0.0 9.803212 2 TCAATAC 205 4.1836756E-11 9.732791 3 AGGACGT 1345 0.0 9.607018 5 TTAGGAC 990 0.0 9.597029 3 ACTAGAC 130 4.2337797E-6 9.501059 3 >>END_MODULE