FastQCFastQC Report
Thu 26 May 2016
SRR1512084_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512084_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1604098
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT60260.37566283356752517No Hit
TATCAACGCAGAGTACTTTTTTTTT38900.2425038869196271No Hit
GGTATCAACGCAGAGTACTTTTTTT37530.23396326159623665No Hit
GTCCTAAAGTGTGTATTTCTCATTT17410.10853451597096936No Hit
GTCCTACAGTGGACATTTCTAAATT17310.10791111266269268No Hit
CTGTAGGACGTGGAATATGGCAAGA17200.10722536902358834No Hit
ACGCAGAGTACTTTTTTTTTTTTTT17010.10604090273786265No Hit
GTACTTTTTTTTTTTTTTTTTTTTT16690.10404601215137728No Hit
CTTTAGGACGTGAAATATGGCGAGG16560.1032355878506176No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGT1301.4551915E-1113.0926237
CAAGTCG1353.092282E-1112.6077126
GTCTAGA1205.487891E-912.3659261
AACCGTC550.003177602312.0346357
GTACTAG853.520842E-511.6385191
TCCAACG1159.357209E-710.68948118
AGTCGTC1605.8935257E-1010.6377568
GCCTCGA1002.5251833E-510.40136216
GGTATCA14000.010.3873781
CCAACGA1106.387985E-610.31572219
CCTCGAA1201.6166232E-610.24408417
ATCGGTC750.00275209710.08616914
TACACGG750.00275209710.0861695
TCGTCAA1701.6898412E-910.01200710
GTGTAGA2001.2187229E-109.8927411
GCTATAT800.00323086059.892741
GCCCTAC1201.0514143E-59.892741
CGTTTCC1151.0846883E-59.86690514
TGGCGAG10900.09.80309118
GGCGAGG6000.09.77128119
Produced by FastQC (version 0.11.5)