FastQCFastQC Report
Thu 26 May 2016
SRR1512084_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512084_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1604098
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT80170.4997824322454114No Hit
TATCAACGCAGAGTACTTTTTTTTT54710.34106394995816963No Hit
GGTATCAACGCAGAGTACTTTTTTT52260.32579056890539104No Hit
ACGCAGAGTACTTTTTTTTTTTTTT24950.15553912541503076No Hit
GTACTTTTTTTTTTTTTTTTTTTTT20970.13072767374561903No Hit
GTCCTAAAGTGTGTATTTCTCATTT18770.11701280096353214No Hit
CTGTAGGACGTGGAATATGGCAAGA18600.1159530153394618No Hit
GTCCTACAGTGGACATTTCTAAATT18540.1155789733544958No Hit
CTTTAGGACGTGAAATATGGCGAGG18310.11414514574545945No Hit
GAGTACTTTTTTTTTTTTTTTTTTT16770.10454473479799863No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCCA707.2897765E-613.5665759
CCGGTAC500.00149728513.3027093
TATGCCG550.003063845712.09336955
TAGGACC2800.011.8774174
GGACTTA752.080193E-411.3962786
GGTATCA15400.011.3340311
TATACTC1354.7111826E-911.2615525
TAACACT1658.0035534E-1110.9416214
GTTATAT1201.4474044E-610.3327561
TATACTG2300.010.3281895
AGGACCT6850.010.1261485
GGACCTG6850.09.98214156
GGCGAGG4800.09.89291119
TAGGACT2700.09.8538584
TGGCGAG10450.09.81528218
GGCGAGA6050.09.57568419
GGTTATA800.00436954259.5379291
GTATCAA29850.09.4260931
TGGCAAG9600.09.39826518
GTGTAGG2650.09.3579671
Produced by FastQC (version 0.11.5)