##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512084_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1604098 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.903907990658926 31.0 31.0 34.0 28.0 34.0 2 31.09450170750166 31.0 31.0 34.0 27.0 34.0 3 31.195469977520077 31.0 31.0 34.0 27.0 34.0 4 34.78679170474622 37.0 35.0 37.0 32.0 37.0 5 34.406400980488726 37.0 35.0 37.0 30.0 37.0 6 34.35541220050147 37.0 35.0 37.0 30.0 37.0 7 34.19260045209208 37.0 35.0 37.0 30.0 37.0 8 34.10033676246713 37.0 35.0 37.0 30.0 37.0 9 35.60318010495618 39.0 35.0 39.0 30.0 39.0 10 35.4199874321893 39.0 35.0 39.0 30.0 39.0 11 35.40779491028603 39.0 35.0 39.0 30.0 39.0 12 35.237679368716876 39.0 35.0 39.0 29.0 39.0 13 35.203331093237445 39.0 35.0 39.0 29.0 39.0 14 36.226029831095104 39.0 36.0 41.0 29.0 41.0 15 36.15075886884716 39.0 36.0 41.0 28.0 41.0 16 36.0531912638754 39.0 36.0 41.0 28.0 41.0 17 36.005186715524864 39.0 36.0 41.0 27.0 41.0 18 35.9476147966022 39.0 36.0 41.0 27.0 41.0 19 35.97034408122197 39.0 36.0 41.0 27.0 41.0 20 35.871302750829436 39.0 36.0 41.0 27.0 41.0 21 35.750422979144666 39.0 36.0 41.0 27.0 41.0 22 35.63522739882476 39.0 36.0 41.0 27.0 41.0 23 35.69775038682175 39.0 36.0 41.0 27.0 41.0 24 35.589710853077555 39.0 36.0 41.0 27.0 41.0 25 35.16502046633061 39.0 35.0 40.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.22879888100059276 1101 2 0.23493484543366705 1101 3 0.24456563770145223 1101 4 0.2929708583363251 1101 5 0.24596911879095273 1101 6 0.19900698713941978 1101 7 0.05331581663002538 1101 8 -0.008229875455306512 1101 9 0.032488981602739386 1101 10 0.020472100236297308 1101 11 0.006987379451047104 1101 12 -0.018003489083213253 1101 13 0.1838534623816983 1101 14 -0.04224973789374786 1101 15 0.17175003742087114 1101 16 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9 -0.3636109294101786 2313 10 -0.45333260161314115 2313 11 -0.6336012503670787 2313 12 -0.5003682659798869 2313 13 -0.3471100967508747 2313 14 -0.521702708440344 2313 15 -0.4601974207486279 2313 16 -0.36721548995130604 2313 17 -0.6622696981015181 2313 18 -0.3932204135605417 2313 19 -0.43628721163645423 2313 20 -0.33315844406016026 2313 21 -0.35330793292072116 2313 22 -0.4936286152291771 2313 23 -0.3409566483550108 2313 24 -0.3019979742298702 2313 25 -0.37558586570266783 2314 1 -0.14631469490533533 2314 2 -0.25306861968055827 2314 3 -0.2023063815011774 2314 4 -0.07146895427894151 2314 5 -0.2359671123061844 2314 6 -0.3188772497290202 2314 7 -0.3101113915227671 2314 8 -0.30185561246715054 2314 9 -0.3454171932045398 2314 10 -0.6268985217546259 2314 11 -0.4712764727955445 2314 12 -0.4734634888350797 2314 13 -0.4226148724732752 2314 14 -0.3815893665251693 2314 15 -0.4495643560126368 2314 16 -0.5072885304115999 2314 17 -0.3960945906507263 2314 18 -0.41019858676671106 2314 19 -0.47881413656432414 2314 20 -0.6123580334594507 2314 21 -0.3673808453646217 2314 22 -0.6069989784875247 2314 23 -0.3522920252091737 2314 24 -0.4633881136491951 2314 25 -0.6011868907820244 2315 1 -0.5304786977085953 2315 2 -0.5720132577199202 2315 3 -0.6352266495780263 2315 4 -0.6206603542264091 2315 5 -0.8502925446267042 2315 6 -0.6710543150452111 2315 7 -0.6061306657496033 2315 8 -0.7254341019264814 2315 9 -0.9138676901295426 2315 10 -0.9181440236482743 2315 11 -0.8230928423320023 2315 12 -0.9467356894787642 2315 13 -0.881160275962344 2315 14 -1.0395214661498287 2315 15 -1.0438058115774567 2315 16 -1.126597737473972 2315 17 -1.0206496215701009 2315 18 -1.0818123130567159 2315 19 -1.0074077498282463 2315 20 -0.9871027927797442 2315 21 -0.8213534010152657 2315 22 -1.0647521826885367 2315 23 -0.928357076329938 2315 24 -0.8870555666649409 2315 25 -1.1183895376596453 2316 1 -1.0615109697356253 2316 2 -1.1525950059199204 2316 3 -1.2562432603170635 2316 4 -1.1921146898201158 2316 5 -1.3535242851473157 2316 6 -1.3356938959835887 2316 7 -1.465446688666745 2316 8 -1.5548895405588965 2316 9 -1.640952293663922 2316 10 -1.803509445944556 2316 11 -1.8059800345167218 2316 12 -1.6540621703198681 2316 13 -1.7631956383594272 2316 14 -1.7214536222879815 2316 15 -1.8766954472023514 2316 16 -1.860553062079184 2316 17 -2.1624087123311 2316 18 -1.751914728106442 2316 19 -1.690079337505864 2316 20 -1.7240664576607614 2316 21 -1.8715655148935397 2316 22 -1.8828752948401188 2316 23 -1.7743813664523884 2316 24 -1.898301790968091 2316 25 -2.0475931855994745 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 23110.0 3 2.0 4 425.0 5 3687.0 6 4816.0 7 3916.0 8 3169.0 9 2850.0 10 2692.0 11 2498.0 12 2463.0 13 2372.0 14 2455.0 15 2338.0 16 2543.0 17 2558.0 18 2874.0 19 3153.0 20 3740.0 21 4910.0 22 6388.0 23 8268.0 24 9951.0 25 11838.0 26 13926.0 27 15892.0 28 18819.0 29 23006.0 30 29580.0 31 37864.0 32 49227.0 33 64074.0 34 83336.0 35 110993.0 36 160799.0 37 278046.0 38 605520.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.5421572696897 20.018544152550387 14.128803601647563 24.310494976112352 2 14.969845981978658 21.62012545368176 40.016258358279856 23.39377020605973 3 18.011430723060563 25.699926064367638 31.032330942373843 25.256312270197956 4 12.727900664423247 18.192155342129972 38.36118491513611 30.718759078310676 5 13.074115241584675 38.023497916050715 35.797957342974016 13.104429499390596 6 30.578493333948426 38.05278729853164 18.330550876567393 13.038168490952549 7 26.819059683385927 32.665211227742944 22.839876366655904 17.675852722215225 8 24.872446434983843 36.37658101584008 21.131186908989346 17.61978564018673 9 26.0711627344464 15.593373970916987 20.83688153716294 37.49858175747367 10 15.106745348476217 29.214175193784918 34.38649010222568 21.292589355513194 11 33.023792810663686 23.206188150599278 25.12870161299372 18.641317425743313 12 22.790315803647907 25.447011342199794 31.816946346170873 19.945726507981433 13 28.14902830126339 22.523000465058868 26.57175559099257 22.756215642685174 14 21.060995026488406 22.577859956187215 28.27776108442252 28.083383932901857 15 22.786887085452385 30.650870458039346 24.38267487397902 22.17956758252925 16 21.69992107714117 28.736897620968293 27.9138182330506 21.649363068839932 17 21.22183308002379 28.84019554914974 28.281501504272182 21.656469866554286 18 20.979578554427473 27.80397457013225 30.3220252129234 20.89442166251688 19 22.7610158481589 27.331808904443495 28.617640568094966 21.289534679302633 20 22.79823302566302 27.527308181919057 29.086813897903994 20.587644894513925 21 22.53035662409653 27.02902191761351 28.501812233417162 21.938809224872795 22 22.613144583435677 27.779848862101943 28.502186275402124 21.104820279060256 23 21.98014086421154 27.5965682894686 28.768691189690404 21.654599656629458 24 22.32057246240379 27.151627974249664 29.181468038388598 21.346331524957947 25 22.2658127549376 27.397512201781844 28.977805817580165 21.358869225700392 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1576.0 1 1576.0 2 1290.5 3 1005.0 4 1005.0 5 1005.0 6 2148.0 7 3291.0 8 3291.0 9 3291.0 10 4384.0 11 5477.0 12 5477.0 13 5477.0 14 7726.0 15 9975.0 16 9975.0 17 9975.0 18 17042.5 19 24110.0 20 24110.0 21 24110.0 22 36146.5 23 48183.0 24 48183.0 25 48183.0 26 67139.5 27 86096.0 28 86096.0 29 86096.0 30 107276.0 31 128456.0 32 128456.0 33 128456.0 34 142671.0 35 156886.0 36 156886.0 37 156886.0 38 168036.5 39 179187.0 40 179187.0 41 179187.0 42 188824.5 43 198462.0 44 198462.0 45 198462.0 46 200329.5 47 202197.0 48 202197.0 49 202197.0 50 195215.5 51 188234.0 52 188234.0 53 188234.0 54 167969.0 55 147704.0 56 147704.0 57 147704.0 58 125826.5 59 103949.0 60 103949.0 61 103949.0 62 83788.5 63 63628.0 64 63628.0 65 63628.0 66 48509.0 67 33390.0 68 33390.0 69 33390.0 70 23946.0 71 14502.0 72 14502.0 73 14502.0 74 9973.0 75 5444.0 76 5444.0 77 5444.0 78 3580.0 79 1716.0 80 1716.0 81 1716.0 82 1101.5 83 487.0 84 487.0 85 487.0 86 305.0 87 123.0 88 123.0 89 123.0 90 69.5 91 16.0 92 16.0 93 16.0 94 10.0 95 4.0 96 4.0 97 4.0 98 2.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4257221192221423 2 0.0 3 0.0 4 0.0 5 0.05560757509827953 6 0.0 7 0.0 8 0.004114461834626064 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.004987226466213411 25 0.0018078695940023615 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1604098.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.50250186508003 #Duplication Level Percentage of deduplicated Percentage of total 1 82.46441828607318 59.788766405887614 2 12.344870323423692 17.90067967296395 3 2.9132932844148995 6.336631553604493 4 1.0770931272217783 3.123677858610475 5 0.42385122337675596 1.5365137056694855 6 0.21819822289523944 0.9491950237451549 7 0.13600840911041795 0.6902664954636252 8 0.08392412745372241 0.48677673657909787 9 0.06095084014166222 0.39771795609441546 >10 0.2453728486662455 3.0763922471607748 >50 0.016050431685493683 0.8180362958905644 >100 0.013221843769253833 1.9125138793181051 >500 0.0017168948548477068 0.8397364971165969 >1k 7.726026846814682E-4 0.9783440556015259 >5k 2.5753422822715605E-4 1.1647516162940998 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 8017 0.4997824322454114 No Hit TATCAACGCAGAGTACTTTTTTTTT 5471 0.34106394995816963 No Hit GGTATCAACGCAGAGTACTTTTTTT 5226 0.32579056890539104 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2495 0.15553912541503076 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2097 0.13072767374561903 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1877 0.11701280096353214 No Hit CTGTAGGACGTGGAATATGGCAAGA 1860 0.1159530153394618 No Hit GTCCTACAGTGGACATTTCTAAATT 1854 0.1155789733544958 No Hit CTTTAGGACGTGAAATATGGCGAGG 1831 0.11414514574545945 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1677 0.10454473479799863 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 6.234033082766763E-5 0.0 3 0.0 0.0 0.0 6.234033082766763E-5 0.0 4 0.0 0.0 0.0 6.234033082766763E-5 0.0 5 0.0 1.2468066165533526E-4 0.0 6.234033082766763E-5 0.0 6 0.0 1.2468066165533526E-4 0.0 6.234033082766763E-5 0.0 7 6.234033082766763E-5 1.2468066165533526E-4 0.0 6.234033082766763E-5 0.0 8 6.234033082766763E-5 1.2468066165533526E-4 0.0 6.234033082766763E-5 6.234033082766763E-5 9 6.234033082766763E-5 1.2468066165533526E-4 0.0 6.234033082766763E-5 1.2468066165533526E-4 10 6.234033082766763E-5 1.2468066165533526E-4 0.0 6.234033082766763E-5 1.2468066165533526E-4 11 6.234033082766763E-5 1.2468066165533526E-4 0.0 6.234033082766763E-5 1.2468066165533526E-4 12 6.234033082766763E-5 1.2468066165533526E-4 0.0 6.234033082766763E-5 1.2468066165533526E-4 13 6.234033082766763E-5 1.2468066165533526E-4 0.0 6.234033082766763E-5 1.2468066165533526E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGTCCA 70 7.2897765E-6 13.566575 9 CCGGTAC 50 0.001497285 13.302709 3 TATGCCG 55 0.0030638457 12.0933695 5 TAGGACC 280 0.0 11.877417 4 GGACTTA 75 2.080193E-4 11.396278 6 GGTATCA 1540 0.0 11.334031 1 TATACTC 135 4.7111826E-9 11.261552 5 TAACACT 165 8.0035534E-11 10.941621 4 GTTATAT 120 1.4474044E-6 10.332756 1 TATACTG 230 0.0 10.328189 5 AGGACCT 685 0.0 10.126148 5 GGACCTG 685 0.0 9.9821415 6 GGCGAGG 480 0.0 9.892911 19 TAGGACT 270 0.0 9.853858 4 TGGCGAG 1045 0.0 9.815282 18 GGCGAGA 605 0.0 9.575684 19 GGTTATA 80 0.0043695425 9.537929 1 GTATCAA 2985 0.0 9.426093 1 TGGCAAG 960 0.0 9.398265 18 GTGTAGG 265 0.0 9.357967 1 >>END_MODULE