FastQCFastQC Report
Thu 26 May 2016
SRR1512083_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512083_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2241447
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT48060.21441506312663206No Hit
TATCAACGCAGAGTACTTTTTTTTT25390.11327504063223445No Hit
GGTATCAACGCAGAGTACTTTTTTT25120.11207046162590505No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13200.011.1903361
GTCTAGA2150.011.066331
GATAGTA650.00776173610.676172
CCGTGCG901.0002723E-410.5040019
ACCGTGC1651.0113581E-910.3130198
TAGGACC3250.010.18084
GTCTTAT2051.8189894E-1110.1553811
GTAGGAC9950.010.0711733
GCACCGT951.7278234E-49.9511596
AGGACGT9850.09.8854915
GTATCAA24900.09.833961
TAGGACG10150.09.6864484
TGTAGGA10550.09.5847012
GTCCTAC10050.09.3710521
CGTAGGA850.00523934939.3304352
GACGTGG5200.09.27187
GGACGTG10000.09.2645286
GTGGTAT6050.09.1762121
TGGTATC5650.09.1240092
TTAGGGT1357.2875755E-69.1034684
Produced by FastQC (version 0.11.5)