FastQCFastQC Report
Thu 26 May 2016
SRR1512083_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512083_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2241447
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT54790.24444031021032395No Hit
TATCAACGCAGAGTACTTTTTTTTT31470.14040037529328153No Hit
GGTATCAACGCAGAGTACTTTTTTT30570.13638511193885022No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11500.012.1115681
TAGGACC4100.011.5878434
GTCCTAC10250.010.6102691
GTATCAA25450.010.4208331
GGCGAGG4800.010.09095419
GTCCTAG2450.09.7346281
GACGTGA5100.09.6831587
ACGTGAA6100.09.6526318
GTCTTAA2106.184564E-119.5399341
TTAGGAC6300.09.5020323
TCCTACA11800.09.5020312
TACACCG900.00111174189.5020315
GTCCTAA4950.09.4435711
TGGCGAG10000.09.40218418
GTATAAG2650.09.3599361
TATGGCG10750.09.27546416
GTACTAA1356.493956E-69.1866041
ACTAGAC1451.7017574E-69.1743763
CGTGAAA6250.09.1164569
GTATTAC1803.941932E-89.0099381
Produced by FastQC (version 0.11.5)