FastQCFastQC Report
Thu 26 May 2016
SRR1512082_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512082_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1944366
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT52660.27083378335148833No Hit
GGTATCAACGCAGAGTACTTTTTTT33480.17218980377151216No Hit
TATCAACGCAGAGTACTTTTTTTTT32600.16766390689818686No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11350.012.5126011
TAGGACC3500.011.0806744
CAGGACG600.00606824111.0356314
TACACCG700.001546332810.8104135
GGCGAGG4250.010.23809819
GGACCGT750.002744525210.089726
GTCCTAC9250.010.0222921
GTCCTAA5500.09.8623621
TCCTACA10750.09.816492
GTATCAA21600.09.7253851
TGGCGAG9150.09.71755718
GTACTAA1552.4751898E-79.5442211
GTTAGAC900.0011585829.4591123
GTGCTAG2103.2923708E-109.3927251
AGGACGT9200.09.3562965
CGTGGAA4550.09.3551669
GGACGTG8800.09.3516236
GTCTAAG2551.8189894E-129.2822231
GTCTAGA2353.092282E-119.232851
TGTAGGA10900.09.2289982
Produced by FastQC (version 0.11.5)