FastQCFastQC Report
Thu 26 May 2016
SRR1512082_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512082_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1944366
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT64230.33033904110645834No Hit
TATCAACGCAGAGTACTTTTTTTTT47760.2456327666704725No Hit
GGTATCAACGCAGAGTACTTTTTTT45540.23421516319458374No Hit
ACGCAGAGTACTTTTTTTTTTTTTT22370.11505035574578036No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCCG456.7604234E-414.7786395
GGTATCA12950.013.3993511
CCCGTAT550.002987525712.13437751
TAGGACC3300.011.8037184
GGCGAGG4000.011.39714619
CTTATAC2200.010.3642413
AACCGTG856.6112913E-410.05604657
AAGTCGT856.6112913E-410.05604657
TCGTCAA1151.0299384E-59.91030610
TTAGGAC6850.09.7085943
GTATCAA26600.09.6775241
TGTACCG900.0011131859.5005535
CTAGCAC1804.1782187E-99.5005533
TGGCGAG9200.09.49762218
ATGCCGG1606.6605935E-89.49762219
CGTGCAC900.00111659449.49737710
CAAGTCG800.0045266099.4973766
CGTCAAG1201.706102E-59.49737611
GATATAC4200.09.307151
GACGTGA4900.09.3035537
Produced by FastQC (version 0.11.5)