FastQCFastQC Report
Thu 26 May 2016
SRR1512081_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512081_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2341349
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT52530.22435783815227886No Hit
GGTATCAACGCAGAGTACTTTTTTT33060.14120064971091453No Hit
TATCAACGCAGAGTACTTTTTTTTT32150.13731400145813374No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCG500.001555677913.2357495
GGTATCA12150.012.8040381
ACCGCTC751.5548387E-512.6054758
TAGGACC4500.011.9752024
GTCTAGT1501.0622898E-911.2300381
GTATCAA25100.010.3431091
TGGCGAG8150.010.20811618
GTAGGAC9750.010.1813463
CTATAGG2500.09.9088571
TGTAGGA10650.09.7692962
TATACTG3350.09.5952135
AGGACGT8050.09.5128285
GGCGAGG4300.09.45410719
GGACGTG7800.09.4541066
GTGGTAT4850.09.3980911
ATGGCGA9000.09.34906117
TCTAATA2251.1459633E-109.2482672
GTCCTAC7500.09.1161481
TAGGACG8700.09.0194364
TATGGCG8600.09.01438116
Produced by FastQC (version 0.11.5)