FastQCFastQC Report
Thu 26 May 2016
SRR1512081_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512081_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2341349
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT64190.274158188292305No Hit
TATCAACGCAGAGTACTTTTTTTTT43080.1839964909118632No Hit
GGTATCAACGCAGAGTACTTTTTTT41750.17831600500395284No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13800.011.6794971
TGTATCG600.00586679311.0859645
CCCGTCC600.00589203411.0795728
TAGGACC3800.011.002614
GACCCGT750.002655898210.1298936
ATTATAC1803.929017E-1010.0301583
GTATCAA27700.09.9846931
GTCCTAC8800.09.9706081
TATGGCG9100.09.70528816
TGTAGGA10050.09.549532
TGGCGAG9150.09.54907818
GTATTAA2600.09.5371031
TCCTATA2700.09.50225452
GACGTGA4100.09.4967757
GTATTGG2550.09.3501011
TAGGACT2850.09.3355484
ATAGGAC2650.09.3229673
TAGGACG8400.09.27601054
TCCTACA9500.09.1021592
GTAGGAC8900.09.0751873
Produced by FastQC (version 0.11.5)