FastQCFastQC Report
Thu 26 May 2016
SRR1512080_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512080_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2413230
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT76020.3150134881465919No Hit
TATCAACGCAGAGTACTTTTTTTTT50490.20922166556855337No Hit
GGTATCAACGCAGAGTACTTTTTTT48620.20147271499194025No Hit
GTCCTAAAGTGTGTATTTCTCATTT31190.12924586549976588No Hit
GTCCTACAGTGGACATTTCTAAATT30210.1251849181387601No Hit
CTTTAGGACGTGAAATATGGCGAGG29600.12265718559772587No Hit
CTGTAGGACGTGGAATATGGCAAGA28110.11648288807946197No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16350.013.0827381
TAGGACC6350.012.9535234
CTGGTCG751.5537493E-512.6063729
GGCGAGG9600.012.01544819
GTCCTAT2650.011.2108361
CGTTAGG802.5682378E-411.1407691
TCCAACG2350.010.86293718
GCGTGCG700.001552487410.8054629
TATGTCG1053.6721794E-610.80546116
CTCAACG650.00782439310.6646672
CGTGCGC803.9423004E-410.63662610
GACAACG909.992921E-510.5050927
TTAGGAC13550.010.4663283
GTGCGCC1002.5295958E-510.40025711
AGGACGT19650.010.3447095
GCGCCAC1401.03918865E-710.1303313
GTCCTAA11000.010.0829581
CCAACGA1606.679329E-910.04570319
GTATCAA32800.09.963291
CGGTAGG800.00320454979.9029051
Produced by FastQC (version 0.11.5)