FastQCFastQC Report
Thu 26 May 2016
SRR1512080_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512080_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2413230
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT96850.40132933868715376No Hit
TATCAACGCAGAGTACTTTTTTTTT67510.27974954728724577No Hit
GGTATCAACGCAGAGTACTTTTTTT63830.26450027556428524No Hit
CTTTAGGACGTGAAATATGGCGAGG33530.13894241328012663No Hit
GTCCTACAGTGGACATTTCTAAATT33100.13716056902988938No Hit
GTCCTAAAGTGTGTATTTCTCATTT32960.13658043369260284No Hit
CTGTAGGACGTGGAATATGGCAAGA32380.13417701586670147No Hit
ACGCAGAGTACTTTTTTTTTTTTTT30070.12460478280147355No Hit
GTACTTTTTTTTTTTTTTTTTTTTT27200.11271200838709945No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18050.013.050671
TCCAACG1900.011.99659818
GGCGAGG9100.011.89821719
GCCGGTT1657.2759576E-1211.51069411
CGGTTTC1501.7826096E-1011.39558713
TAGGACC6600.011.0863924
CCAACGA1659.331416E-1010.36112719
CCGTGTA856.6192733E-410.054939
TGGCGAG19600.09.83649618
AATCCCG3100.09.80407619
ATTTAGA8000.09.6562411
TGTCCAC6900.09.49632310
GTCCTAA11550.09.4957421
TTAGGAC14550.09.4699683
GTATCAA34350.09.3010321
ATCCCGT1856.7448127E-99.23966510
CCGGTTT1856.7448127E-99.23966512
GACGTGA12050.09.2210697
GGACGTG20500.09.2189546
AGGACGT20600.09.1797185
Produced by FastQC (version 0.11.5)