FastQCFastQC Report
Thu 26 May 2016
SRR1512079_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512079_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4122098
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT68530.16625029293335578No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20250.011.3413891
AATCCCG3850.011.29714819
CCTAGAC3850.010.5602493
GTCCTAG4900.010.505351
GTCCTAA7350.010.2359821
TAGGACC5600.09.9616324
TAACGCT951.7258448E-49.95274454
GTATCAA39750.09.8370121
TCACGAC1356.88251E-79.8052973
TAGGACA7050.09.65628054
GGTCGAC1304.5067263E-69.4551087
TGGACCG1901.1477823E-99.4551085
ATCGCTC1002.8916157E-49.4551088
ACCGTGC2305.456968E-129.4551078
TGGCGAG9750.09.406733518
TCCAACG2750.09.28330918
GTAGGAC12150.09.2605583
AGGACGT9750.09.2126695
CTAGGAC4350.09.1290693
ATTATAC4100.08.9938823
Produced by FastQC (version 0.11.5)