##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512079_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4122098 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.194147737390036 31.0 28.0 33.0 16.0 34.0 2 25.717897294047837 31.0 24.0 33.0 7.0 34.0 3 27.342387298894884 31.0 26.0 34.0 16.0 34.0 4 31.520720516591307 35.0 32.0 37.0 19.0 37.0 5 31.88620163809788 35.0 32.0 37.0 19.0 37.0 6 32.11588540592679 35.0 33.0 37.0 19.0 37.0 7 32.12997192206493 35.0 33.0 37.0 19.0 37.0 8 32.1568274213762 35.0 33.0 37.0 19.0 37.0 9 33.6192970181689 38.0 34.0 39.0 21.0 39.0 10 33.46607334420482 38.0 33.0 39.0 19.0 39.0 11 33.476465382433894 38.0 34.0 39.0 19.0 39.0 12 33.347565729878326 38.0 33.0 39.0 18.0 39.0 13 33.33257506250458 38.0 33.0 39.0 17.0 39.0 14 34.32459368991227 39.0 34.0 41.0 17.0 41.0 15 34.23625129727629 39.0 33.0 41.0 17.0 41.0 16 34.18767676071748 39.0 33.0 41.0 17.0 41.0 17 34.103432523923495 39.0 33.0 41.0 17.0 41.0 18 34.04220496455931 39.0 33.0 41.0 16.0 41.0 19 34.047248512771894 39.0 34.0 41.0 15.0 41.0 20 33.954264066502056 39.0 34.0 41.0 11.0 41.0 21 33.87499909026908 39.0 34.0 41.0 10.0 41.0 22 33.746394918315865 39.0 33.0 41.0 8.0 41.0 23 33.61386798664175 39.0 33.0 41.0 2.0 41.0 24 33.473954039908804 39.0 33.0 41.0 2.0 41.0 25 33.32575062504579 39.0 33.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.953643014974773 1101 2 3.190994392365514 1101 3 1.8752534537819656 1101 4 1.2315394698185287 1101 5 0.6486523774820547 1101 6 0.40571981448102434 1101 7 0.35755200081185734 1101 8 0.24511924113804184 1101 9 0.31268566675071696 1101 10 0.15338023887946406 1101 11 0.47167377253261833 1101 12 0.1928111894059512 1101 13 0.2693168597246327 1101 14 0.29704368783454527 1101 15 0.3803238201778356 1101 16 0.30879064710998705 1101 17 0.09708984090681838 1101 18 0.09838372264208317 1101 19 0.0872349616758541 1101 20 0.1798981904141499 1101 21 0.17935208850239093 1101 22 0.1610589378022098 1101 23 0.2825394720548431 1101 24 0.20512267553439045 1101 25 0.035612276792392095 1102 1 1.30978425379163 1102 2 3.714889723604877 1102 3 2.3463432613053747 1102 4 1.4064785610749624 1102 5 1.1809377330849138 1102 6 1.1327494029810552 1102 7 0.9668140994468075 1102 8 0.8610820458678319 1102 9 0.7685386124513798 1102 10 0.9555898245015229 1102 11 1.0421927001417686 1102 12 0.9576295051985539 1102 13 0.8630346996599272 1102 14 0.8209509279117526 1102 15 0.9534078618389188 1102 16 1.0481138777792083 1102 17 0.7236949289577908 1102 18 0.796711771428626 1102 19 0.9709054639807064 1102 20 1.069913126081616 1102 21 0.9578548994843246 1102 22 1.0411260242554121 1102 23 0.85817746323238 1102 24 0.737230639073502 1102 25 0.7651563667835077 1103 1 0.9323433753097206 1103 2 2.7698722366963224 1103 3 1.736488677490435 1103 4 1.093460915052482 1103 5 0.7189488955755614 1103 6 0.6374172454563478 1103 7 0.4321540035602425 1103 8 0.2660062492346569 1103 9 0.515077644274406 1103 10 0.34540637720464673 1103 11 0.5027269191291523 1103 12 0.4232407081647125 1103 13 0.49934329510460884 1103 14 0.5405783153116133 1103 15 0.44662545060922554 1103 16 0.47985857155821776 1103 17 0.46512531024714576 1103 18 0.42165141269162376 1103 19 0.5775831772876998 1103 20 0.670730243374777 1103 21 0.7841759836894795 1103 22 0.7044647583286974 1103 23 0.7993659408846838 1103 24 0.6487106605418589 1103 25 0.6664040650996697 1104 1 1.401467910748984 1104 2 3.772691412507047 1104 3 2.404142353567078 1104 4 1.8187180737752442 1104 5 1.363246561922164 1104 6 1.1026434222807069 1104 7 1.0175425987326108 1104 8 0.9087564238485086 1104 9 0.9656162470441103 1104 10 0.9840195557516012 1104 11 0.9971828883816798 1104 12 1.0066766207677063 1104 13 0.9602432403409011 1104 14 1.0853266377093291 1104 15 0.8344618995794377 1104 16 1.0277093589638397 1104 17 0.9497517203174937 1104 18 1.0308692548402263 1104 19 0.9664084367799504 1104 20 0.8716649967840269 1104 21 1.0038848958749966 1104 22 0.948461132274474 1104 23 1.170644370820071 1104 24 1.143002451502582 1104 25 1.1523638522842248 1105 1 1.2413751873554268 1105 2 3.6193664442936964 1105 3 2.157868453697244 1105 4 1.500702329983767 1105 5 1.1862756197740403 1105 6 0.8488831200492868 1105 7 0.5813986127229995 1105 8 0.5710360088676225 1105 9 0.5938626684621298 1105 10 0.6383446187404687 1105 11 0.33638199723844053 1105 12 0.3788659594545294 1105 13 0.31677394732136577 1105 14 0.32611156096245963 1105 15 0.4884061906902204 1105 16 0.5587826589034819 1105 17 0.6134466619879362 1105 18 0.4616310738254299 1105 19 0.7184920875191523 1105 20 0.7803817189744109 1105 21 0.7195613310993778 1105 22 0.7743309798056472 1105 23 0.6716349650119042 1105 24 0.8172220246165978 1105 25 0.9211297794369742 1106 1 0.8169826442983457 1106 2 3.1734869486687174 1106 3 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-2.9653273989964752 2316 1 -1.967727543400862 2316 2 -2.2599193132182442 2316 3 -1.7960155817510284 2316 4 -2.272826012414974 2316 5 -2.1729719880001817 2316 6 -2.461843637295626 2316 7 -2.418539369746515 2316 8 -2.525489895917797 2316 9 -2.732441236802579 2316 10 -2.8313405733032795 2316 11 -3.1193414558937818 2316 12 -3.056833480644812 2316 13 -3.083728825554555 2316 14 -3.3308750938913434 2316 15 -3.6129756722079485 2316 16 -3.509128134615903 2316 17 -3.3693568596007957 2316 18 -3.10745384080969 2316 19 -3.4446432109129823 2316 20 -3.493756631920874 2316 21 -3.5303941043910108 2316 22 -3.4925451231114977 2316 23 -3.44400875129541 2316 24 -3.3754357001001267 2316 25 -3.7009867079791796 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 238154.0 3 7.0 4 1957.0 5 9647.0 6 11485.0 7 10175.0 8 9478.0 9 9152.0 10 8501.0 11 8485.0 12 8264.0 13 8276.0 14 8401.0 15 8652.0 16 9055.0 17 9802.0 18 11156.0 19 13959.0 20 17964.0 21 23024.0 22 28027.0 23 33437.0 24 38281.0 25 43243.0 26 48352.0 27 54733.0 28 62570.0 29 74211.0 30 90745.0 31 112744.0 32 140298.0 33 179781.0 34 233514.0 35 310934.0 36 435691.0 37 598349.0 38 1211594.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.74672800112952 18.16749626039944 13.069776604049684 25.015999134421357 2 16.6206173804687 20.928473495371957 38.56532470969646 23.88558441446288 3 18.948433540396177 23.824542744980835 29.344256250094002 27.882767464528985 4 12.791787094824045 16.430080022357547 37.371479280696384 33.40665360212203 5 14.069583013310213 37.20767919637039 34.92524922988245 13.797488560436943 6 32.73794072824081 36.21510211547615 17.686673145568108 13.360284010714931 7 28.781266238696894 31.473827162770025 21.379331592795708 18.36557500573737 8 26.077670383393265 35.871425292922076 19.985099777151667 18.065804546532995 9 26.41106543318475 15.89610436239022 19.64181831678917 38.05101188763586 10 15.374622340371335 28.870759501593607 33.372423460092406 22.38219469794265 11 35.02898281409127 22.676898996578927 23.38527613851005 18.90884205081975 12 23.290227452137238 24.42777925221574 30.752422674084894 21.529570621562126 13 28.746381090405905 21.36834204329931 25.644999221270332 24.240277645024452 14 21.884130679276446 20.994703660180317 27.070210951138403 30.050954709404838 15 23.757974701232236 28.97349844666478 23.313783418055564 23.954743434047423 16 23.511765125428845 27.85634887865354 25.73563268025166 22.896253315665955 17 22.757858439527258 27.667374154465556 26.674699794788914 22.90006761121827 18 22.53340410635555 27.52302347008732 27.658173095350957 22.28539932820617 19 23.830889546969775 26.8717278407758 26.917651180093966 22.379731432160458 20 23.862105806571815 26.811193744529472 27.17098713079391 22.155713318104805 21 24.01021228035446 26.311347431015676 26.696758154104128 22.981682134525734 22 23.784155153039542 26.5558437601284 26.838298797303878 22.82170228952818 23 23.689670857517147 26.374435205570013 26.69817992152011 23.237714015392733 24 23.35765839003398 26.557760668321656 27.042879007648313 23.041701933996055 25 23.572104198421336 26.495955820544737 26.918265145143355 23.01367483589057 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 287.0 1 287.0 2 578.5 3 870.0 4 870.0 5 870.0 6 2156.5 7 3443.0 8 3443.0 9 3443.0 10 5288.5 11 7134.0 12 7134.0 13 7134.0 14 11908.0 15 16682.0 16 16682.0 17 16682.0 18 29513.0 19 42344.0 20 42344.0 21 42344.0 22 65061.5 23 87779.0 24 87779.0 25 87779.0 26 123390.5 27 159002.0 28 159002.0 29 159002.0 30 204674.0 31 250346.0 32 250346.0 33 250346.0 34 293501.0 35 336656.0 36 336656.0 37 336656.0 38 381235.5 39 425815.0 40 425815.0 41 425815.0 42 462870.0 43 499925.0 44 499925.0 45 499925.0 46 521131.5 47 542338.0 48 542338.0 49 542338.0 50 539801.0 51 537264.0 52 537264.0 53 537264.0 54 495537.5 55 453811.0 56 453811.0 57 453811.0 58 394548.5 59 335286.0 60 335286.0 61 335286.0 62 276281.5 63 217277.0 64 217277.0 65 217277.0 66 168260.0 67 119243.0 68 119243.0 69 119243.0 70 86898.0 71 54553.0 72 54553.0 73 54553.0 74 37946.5 75 21340.0 76 21340.0 77 21340.0 78 14421.0 79 7502.0 80 7502.0 81 7502.0 82 4879.5 83 2257.0 84 2257.0 85 2257.0 86 1472.5 87 688.0 88 688.0 89 688.0 90 440.5 91 193.0 92 193.0 93 193.0 94 121.0 95 49.0 96 49.0 97 49.0 98 31.5 99 14.0 100 14.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.488296978868528 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 2.911138939442973E-4 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 1.4555694697214865E-4 15 0.0 16 0.0 17 2.4259491162024774E-5 18 0.0 19 2.6685440278227256E-4 20 2.4259491162024774E-4 21 4.851898232404955E-5 22 6.307467702126442E-4 23 5.579682967265698E-4 24 2.6685440278227256E-4 25 2.4259491162024774E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 4122098.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.70907994202423 #Duplication Level Percentage of deduplicated Percentage of total 1 81.36372425032894 56.71790358146996 2 11.841777543164943 16.509588348243046 3 3.375272443429128 7.058614097553377 4 1.3382107903398235 3.7314177185231276 5 0.6734451118107898 2.34726195678919 6 0.37731340083985365 1.5781302013405436 7 0.23188135565243712 1.1314965170768485 8 0.16206729728470293 0.9038049745925733 9 0.11033231254554013 0.6922047595882915 >10 0.4947015525610853 5.767573064318507 >50 0.019576519124218105 0.9375216163132409 >100 0.010224297525606168 1.288092372425997 >500 7.716372157531128E-4 0.3702471565477178 >1k 6.664137682718977E-4 0.798587458268416 >5k 3.507440885641102E-5 0.1675561769491464 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6853 0.16625029293335578 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 2.4259491162024774E-5 0.0 0.0 0.0 6 0.0 2.4259491162024774E-5 0.0 0.0 0.0 7 0.0 2.4259491162024774E-5 0.0 0.0 0.0 8 2.4259491162024774E-5 2.4259491162024774E-5 0.0 0.0 0.0 9 2.4259491162024774E-5 2.4259491162024774E-5 0.0 0.0 0.0 10 2.4259491162024774E-5 2.4259491162024774E-5 0.0 0.0 0.0 11 2.4259491162024774E-5 2.4259491162024774E-5 0.0 0.0 0.0 12 2.4259491162024774E-5 2.4259491162024774E-5 0.0 0.0 0.0 13 2.4259491162024774E-5 4.851898232404955E-5 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2025 0.0 11.341389 1 AATCCCG 385 0.0 11.297148 19 CCTAGAC 385 0.0 10.560249 3 GTCCTAG 490 0.0 10.50535 1 GTCCTAA 735 0.0 10.235982 1 TAGGACC 560 0.0 9.961632 4 TAACGCT 95 1.7258448E-4 9.9527445 4 GTATCAA 3975 0.0 9.837012 1 TCACGAC 135 6.88251E-7 9.805297 3 TAGGACA 705 0.0 9.6562805 4 GGTCGAC 130 4.5067263E-6 9.455108 7 TGGACCG 190 1.1477823E-9 9.455108 5 ATCGCTC 100 2.8916157E-4 9.455108 8 ACCGTGC 230 5.456968E-12 9.455107 8 TGGCGAG 975 0.0 9.4067335 18 TCCAACG 275 0.0 9.283309 18 GTAGGAC 1215 0.0 9.260558 3 AGGACGT 975 0.0 9.212669 5 CTAGGAC 435 0.0 9.129069 3 ATTATAC 410 0.0 8.993882 3 >>END_MODULE