FastQCFastQC Report
Thu 26 May 2016
SRR1512079_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512079_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4122098
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT83130.20166915002991195No Hit
TATCAACGCAGAGTACTTTTTTTTT53200.1290604929819718No Hit
GGTATCAACGCAGAGTACTTTTTTT51150.12408729729375671No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23500.011.2024761
GTAGGAC12000.010.3725023
TAGGACG10200.010.3398994
TAGGACC6200.010.2677814
TACGACT856.588113E-410.0604434
TTAGGAC8600.010.0539453
TATGGCG9900.09.78446716
GGACGTG10250.09.7286696
GACGTGA6250.09.7249637
CTAGACA5300.09.6808034
GTATCAA44900.09.6233141
TGGCGAG10000.09.59212118
ATGGCGA10450.09.54212817
GTATTAG3400.09.538341
CGTACAC1401.0574968E-69.501533
AGGACGT10900.09.5015295
GGACCGT2054.2564352E-109.2653996
ACCCCGC1752.687375E-89.225698
GTCCTAG5600.09.1976851
AAGCCGT1554.2850115E-79.1906786
Produced by FastQC (version 0.11.5)