FastQCFastQC Report
Thu 26 May 2016
SRR1512078_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512078_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1972493
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT52220.2647411169519993No Hit
GGTATCAACGCAGAGTACTTTTTTT35270.17880925306198805No Hit
TATCAACGCAGAGTACTTTTTTTTT34880.17683205973354532No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11650.011.9889051
TAGGACC3550.011.9845584
GGACGTG10000.011.4399256
AGGACGT10450.011.3996665
CTCAACG650.00780544410.6679882
GACGTGA5750.010.52325257
GTAGGAC10800.010.2423583
TAGGACG11600.010.1065184
GGCGAGG5150.010.09727919
AAACCGA750.00275571410.0847846
TTAGGAC7100.09.9871323
GTCCTAA7100.09.766211
TAGGACT1752.7957867E-99.7246124
TGGCGAG10650.09.5878918
GTACATA4050.09.5388471
CGTGAAA6850.09.5232549
CCCCTAT1157.036309E-59.4750421
GTATCAA22100.09.4575031
TCCAACG1401.1298162E-69.45472418
TGTAGGA11250.09.4216982
Produced by FastQC (version 0.11.5)