FastQCFastQC Report
Thu 26 May 2016
SRR1512078_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512078_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1972493
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT66370.33647774668908836No Hit
TATCAACGCAGAGTACTTTTTTTTT45260.22945582062902123No Hit
GGTATCAACGCAGAGTACTTTTTTT44740.22681956285776428No Hit
ACGCAGAGTACTTTTTTTTTTTTTT20910.10600798076342985No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14800.012.3715651
TAGGACC4100.010.8923384
TCGAACT1053.4749755E-610.85510119
TTAGGAC7800.010.3545543
GTCCTAA6700.010.2480871
GTACATA3650.010.1895931
CCGTGCA951.6504094E-49.9963449
TAATACT2105.456968E-129.9542944
GGACGTG10150.09.918316
GACGTGA5650.09.9174817
GGCGAGG6400.09.64662319
GATCTAT800.0043760269.5364141
TGGCCCG1304.2285355E-69.5018265
GACCGTG1106.8588815E-59.497257
ACAGCGT1106.8588815E-59.497258
TTTAGGA9500.09.4018072
AGGACGT10800.09.3258665
TAGACTG2550.09.31551555
CGTGAAA6650.09.282329
ATAATAC2054.2200554E-109.2700743
Produced by FastQC (version 0.11.5)