FastQCFastQC Report
Thu 26 May 2016
SRR1512077_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512077_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2252709
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT62670.2781983824808264No Hit
GGTATCAACGCAGAGTACTTTTTTT40010.17760838173061855No Hit
TATCAACGCAGAGTACTTTTTTTTT38490.17086095008276703No Hit
GTCCTAAAGTGTGTATTTCTCATTT25060.11124384019418397No Hit
CTGTAGGACGTGGAATATGGCAAGA24000.10653839443976121No Hit
GTCCTACAGTGGACATTTCTAAATT23940.1062720484536618No Hit
CTTTAGGACGTGAAATATGGCGAGG23180.10289833262973602No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCGCC400.005451653614.1814654
TCCAACG1800.012.08050818
TAGGACC5750.012.0028644
ATAATAC2300.011.5095953
GGTATCA14650.011.2923351
CGGTAGG802.5603027E-411.1444181
TTAGGAC9850.011.134013
GACGTGA8300.010.9351067
AACGGAT700.001553059210.8049277
GGCGAGG8250.010.65758719
TAGGACA5800.010.43234354
AGGACGT16200.010.3297095
TGTAGGA16550.010.1156442
GGACGTG16050.010.0728176
GTATAGA3600.09.9061491
ACGTGAA10050.09.8778578
TGGCGAG15800.09.87317218
TCGAACT1252.734254E-69.83248219
TAGGACG17100.09.786044
GTCCTAA8000.09.7823221
Produced by FastQC (version 0.11.5)