##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512077_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2252709 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.35789442844149 31.0 28.0 33.0 16.0 34.0 2 25.818543362680224 31.0 25.0 33.0 7.0 34.0 3 27.477648466801526 31.0 26.0 34.0 16.0 34.0 4 31.705758710956452 35.0 32.0 37.0 19.0 37.0 5 32.090581162502566 35.0 33.0 37.0 19.0 37.0 6 32.337007576211576 35.0 33.0 37.0 22.0 37.0 7 32.369493352226144 35.0 33.0 37.0 22.0 37.0 8 32.40157605798174 36.0 33.0 37.0 23.0 37.0 9 33.884475091989245 38.0 34.0 39.0 23.0 39.0 10 33.719812900822966 38.0 34.0 39.0 22.0 39.0 11 33.72978045544276 38.0 34.0 39.0 23.0 39.0 12 33.61601609440012 38.0 34.0 39.0 22.0 39.0 13 33.60036693598685 38.0 34.0 39.0 21.0 39.0 14 34.59721428733138 39.0 34.0 41.0 19.0 41.0 15 34.515889091755746 39.0 34.0 41.0 19.0 41.0 16 34.46993553095407 39.0 34.0 41.0 18.0 41.0 17 34.40670011084432 39.0 34.0 41.0 18.0 41.0 18 34.353208958636024 39.0 34.0 41.0 17.0 41.0 19 34.363033574243275 39.0 34.0 41.0 18.0 41.0 20 34.27707573414942 39.0 34.0 41.0 18.0 41.0 21 34.200468413807556 39.0 34.0 41.0 17.0 41.0 22 34.07245853769839 39.0 34.0 41.0 11.0 41.0 23 33.94288077155105 39.0 34.0 41.0 10.0 41.0 24 33.80840889790914 39.0 34.0 41.0 2.0 41.0 25 33.66795400559948 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.9387400203712133 1101 2 3.2103769951162775 1101 3 1.6876127587508805 1101 4 1.0913682316759719 1101 5 0.787523449274623 1101 6 0.4231228506782827 1101 7 0.23108141832113915 1101 8 0.13983267783053321 1101 9 0.21547042990579968 1101 10 -0.03871800305754647 1101 11 0.19264343849118148 1101 12 0.07763689768662374 1101 13 0.22215871516529262 1101 14 0.28080643295059105 1101 15 0.2736399097955342 1101 16 0.3913070924707043 1101 17 0.2929199753494487 1101 18 0.1553901525986987 1101 19 0.038882946226280524 1101 20 0.21237227678426507 1101 21 0.21309649260736307 1101 22 0.11612235315965336 1101 23 -0.05584622791449334 1101 24 -0.017456033365988333 1101 25 -0.1473858380496722 1102 1 1.007172744577808 1102 2 3.460526959374427 1102 3 2.1025493771259356 1102 4 1.1839467612507946 1102 5 0.8972061489699712 1102 6 0.6846850281325771 1102 7 0.6727687190001461 1102 8 0.42630719415680574 1102 9 0.4112814134395961 1102 10 0.33616278315626147 1102 11 0.5431894599149061 1102 12 0.465727908387791 1102 13 0.43848895384243747 1102 14 0.5795653171630306 1102 15 0.5109983462097958 1102 16 0.6873661743774875 1102 17 0.45609690910847434 1102 18 0.5724904454858049 1102 19 0.7110775673002436 1102 20 0.74672434205727 1102 21 0.6434061186637976 1102 22 0.7112398021361557 1102 23 0.5446371784177799 1102 24 0.4680063467740183 1102 25 0.41518112221714887 1103 1 0.9000255289873813 1103 2 2.7917837379881227 1103 3 1.8364852443501896 1103 4 1.1026489457047148 1103 5 0.7178062163478884 1103 6 0.5341482392746784 1103 7 0.48382694761409795 1103 8 0.292759265598022 1103 9 0.4446669990344674 1103 10 0.2736813679450094 1103 11 0.35037990393880847 1103 12 0.29699134744600997 1103 13 0.3176592515256331 1103 14 0.3132934214887939 1103 15 0.24662639604882486 1103 16 0.20328914869996595 1103 17 0.32277092374942384 1103 18 0.2339877477909198 1103 19 0.33485097124288643 1103 20 0.46123255202198266 1103 21 0.5703380343107725 1103 22 0.45174587560214974 1103 23 0.48689735514730614 1103 24 0.3974251864644245 1103 25 0.5357867328037358 1104 1 1.3797482740265252 1104 2 3.8656158295993137 1104 3 2.470310106933823 1104 4 1.8035903926732288 1104 5 1.2410623075775504 1104 6 0.9417164621763945 1104 7 0.9129709730194477 1104 8 0.9742068114310172 1104 9 1.0679702379149276 1104 10 1.0888548374138693 1104 11 0.9898340370558287 1104 12 0.9827482805980097 1104 13 0.8756887223633285 1104 14 1.0564263682420716 1104 15 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14 0.29633016007277746 1107 15 0.355996768451071 1107 16 0.33585867135001024 1107 17 0.38181813483701177 1107 18 0.23514002279515722 1107 19 0.3808336672789352 1107 20 0.3393798751992847 1107 21 0.43499410390721494 1107 22 0.5197944760192073 1107 23 0.6634934189904698 1107 24 0.6761769479248727 1107 25 0.8448725588697243 1108 1 0.4834106733878727 1108 2 1.9416860343096758 1108 3 1.031852882705401 1108 4 0.6199056686576654 1108 5 0.47569536072006713 1108 6 0.35996159437849684 1108 7 0.27043102415212417 1108 8 0.35288114253110336 1108 9 0.22292271014775622 1108 10 0.13646462308000906 1108 11 0.14941352430741972 1108 12 0.24201734736028158 1108 13 0.23294294612072264 1108 14 -0.021975895780947496 1108 15 0.15293291386141306 1108 16 0.15774181344048088 1108 17 0.21746645360459382 1108 18 0.33978004813544516 1108 19 0.4005820106535083 1108 20 0.32178114765694943 1108 21 0.4152257271865665 1108 22 0.3218735215550055 1108 23 0.3871135655816431 1108 24 0.3552676910448369 1108 25 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-2.4901323602309873 2315 24 -2.464958239826828 2315 25 -2.577602497800161 2316 1 -1.942020849083221 2316 2 -2.224602643076473 2316 3 -1.6204474389524677 2316 4 -2.075623048435709 2316 5 -1.9823872794800081 2316 6 -2.1325061435024537 2316 7 -2.12572192572393 2316 8 -2.3831447222111173 2316 9 -2.761007240731985 2316 10 -2.910528985965314 2316 11 -3.2726142597395977 2316 12 -2.9664373186971957 2316 13 -3.1530217247258427 2316 14 -3.3704994439626503 2316 15 -3.4140491720155524 2316 16 -3.2301375749508416 2316 17 -3.2287920947330804 2316 18 -3.1718935819745155 2316 19 -3.2884933651797645 2316 20 -3.4000618620184717 2316 21 -3.4141891784353824 2316 22 -3.4052319796569464 2316 23 -3.353241744342043 2316 24 -3.56702982321692 2316 25 -3.884616774951006 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 119341.0 3 5.0 4 1037.0 5 4671.0 6 5791.0 7 5118.0 8 4823.0 9 4616.0 10 4369.0 11 4407.0 12 4191.0 13 4243.0 14 4310.0 15 4384.0 16 4667.0 17 5155.0 18 5745.0 19 7287.0 20 9527.0 21 12101.0 22 14916.0 23 17792.0 24 20594.0 25 23132.0 26 25916.0 27 28968.0 28 33462.0 29 40016.0 30 49129.0 31 60801.0 32 76712.0 33 98236.0 34 128981.0 35 171528.0 36 242138.0 37 330299.0 38 674301.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.15697233863761 18.91198552498348 13.066667732050611 24.8643744043283 2 16.08542239554069 21.74761201305346 39.13707807889381 23.029887512512044 3 18.213670740428526 25.197306886952553 30.266403694396395 26.322618678222533 4 12.49713123177472 17.51943992766043 38.36119090392945 31.6222379366354 5 13.305979600560924 37.815891888388606 35.65791231801356 13.220216193036915 6 30.995747786331922 37.4664015636285 18.57545737154688 12.962393278492696 7 27.30818760878569 32.13126950706904 22.792646542451777 17.76789634169349 8 24.899265549639214 36.61136278385319 21.040171704683925 17.449199961823673 9 26.405319106906394 15.604057159624258 20.544997156756597 37.44562657671275 10 15.281290215469465 28.899871221715724 34.33918894983773 21.479649612977088 11 33.51728962773265 22.75380441947895 25.08020343506418 18.648702517724217 12 23.042834205394485 24.906900980108837 31.842195330155825 20.208069484340854 13 28.658339803321248 22.194611021663253 26.117576659923674 23.02947251509183 14 21.244226907184828 22.208539827015855 27.85227629875722 28.694956967042103 15 22.936340201952405 30.416356484570358 23.875831276920366 22.771472036556876 16 22.16371488727572 28.17754090741414 27.68231493725998 21.976429268050158 17 21.710482800929903 28.416408865947623 27.892506311290095 21.98060202183238 18 21.472103143370937 27.605562902265675 29.68354989481553 21.23878405954786 19 23.30930001518174 27.114013102464323 27.776949144717513 21.799737737636423 20 23.35189469740419 27.355032254513233 28.31569826182532 20.97737478625725 21 22.988188887246423 26.845677803924072 27.547055567319166 22.61907774151033 22 23.11059203099565 27.66591956418578 27.671646018139096 21.551842386679468 23 22.57902846405271 27.434089122346904 27.895579573143532 22.091302840456855 24 22.912621531615756 26.922643256598956 28.37906943915451 21.785665772630782 25 22.799349583101947 27.180474708451026 28.113085178778086 21.907090529668945 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1184.0 1 1184.0 2 1096.0 3 1008.0 4 1008.0 5 1008.0 6 2189.0 7 3370.0 8 3370.0 9 3370.0 10 4678.5 11 5987.0 12 5987.0 13 5987.0 14 9345.5 15 12704.0 16 12704.0 17 12704.0 18 21571.0 19 30438.0 20 30438.0 21 30438.0 22 46101.5 23 61765.0 24 61765.0 25 61765.0 26 86566.5 27 111368.0 28 111368.0 29 111368.0 30 141261.0 31 171154.0 32 171154.0 33 171154.0 34 192388.5 35 213623.0 36 213623.0 37 213623.0 38 229988.0 39 246353.0 40 246353.0 41 246353.0 42 260588.5 43 274824.0 44 274824.0 45 274824.0 46 279709.0 47 284594.0 48 284594.0 49 284594.0 50 277196.5 51 269799.0 52 269799.0 53 269799.0 54 243566.0 55 217333.0 56 217333.0 57 217333.0 58 186743.0 59 156153.0 60 156153.0 61 156153.0 62 127612.0 63 99071.0 64 99071.0 65 99071.0 66 76490.0 67 53909.0 68 53909.0 69 53909.0 70 39051.0 71 24193.0 72 24193.0 73 24193.0 74 16764.5 75 9336.0 76 9336.0 77 9336.0 78 6249.5 79 3163.0 80 3163.0 81 3163.0 82 2076.0 83 989.0 84 989.0 85 989.0 86 648.0 87 307.0 88 307.0 89 307.0 90 185.5 91 64.0 92 64.0 93 64.0 94 40.0 95 16.0 96 16.0 97 16.0 98 10.0 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.562551132880456 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 1.7756399073293533E-4 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 7.546469606149751E-4 15 0.0 16 0.0 17 0.0 18 0.0 19 1.3317299304970148E-4 20 4.439099768323383E-5 21 0.0 22 2.6634598609940295E-4 23 3.5512798146587066E-4 24 1.3317299304970148E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2252709.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.47894475807455 #Duplication Level Percentage of deduplicated Percentage of total 1 82.55823420947819 59.83733696592951 2 11.787527021703035 17.086950396806508 3 3.1603275371749855 6.871716149529828 4 1.0901189956502289 3.160426978618426 5 0.5021811488626005 1.8198779873479416 6 0.2597794527158817 1.1297124361606339 7 0.15879248604197077 0.8056378276683327 8 0.09467408175193523 0.5489502033055959 9 0.07250469542740895 0.47295574331257745 >10 0.28587521400649335 3.5309175682657594 >50 0.015753675772180703 0.7972934107976488 >100 0.01169780015969889 1.6382187566804893 >500 0.0017303189061355624 0.8606377117244247 >1k 7.415652454847926E-4 1.1586696312545708 >5k 6.179710379039938E-5 0.2806982325977964 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6267 0.2781983824808264 No Hit GGTATCAACGCAGAGTACTTTTTTT 4001 0.17760838173061855 No Hit TATCAACGCAGAGTACTTTTTTTTT 3849 0.17086095008276703 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2506 0.11124384019418397 No Hit CTGTAGGACGTGGAATATGGCAAGA 2400 0.10653839443976121 No Hit GTCCTACAGTGGACATTTCTAAATT 2394 0.1062720484536618 No Hit CTTTAGGACGTGAAATATGGCGAGG 2318 0.10289833262973602 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.439099768323383E-5 0.0 5 0.0 0.0 0.0 4.439099768323383E-5 0.0 6 0.0 0.0 0.0 4.439099768323383E-5 0.0 7 0.0 0.0 0.0 4.439099768323383E-5 0.0 8 0.0 0.0 0.0 4.439099768323383E-5 0.0 9 0.0 0.0 0.0 4.439099768323383E-5 0.0 10 0.0 0.0 0.0 4.439099768323383E-5 0.0 11 0.0 0.0 0.0 4.439099768323383E-5 0.0 12 0.0 4.439099768323383E-5 0.0 4.439099768323383E-5 0.0 13 0.0 4.439099768323383E-5 0.0 4.439099768323383E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTGCGCC 40 0.0054516536 14.181465 4 TCCAACG 180 0.0 12.080508 18 TAGGACC 575 0.0 12.002864 4 ATAATAC 230 0.0 11.509595 3 GGTATCA 1465 0.0 11.292335 1 CGGTAGG 80 2.5603027E-4 11.144418 1 TTAGGAC 985 0.0 11.13401 3 GACGTGA 830 0.0 10.935106 7 AACGGAT 70 0.0015530592 10.804927 7 GGCGAGG 825 0.0 10.657587 19 TAGGACA 580 0.0 10.4323435 4 AGGACGT 1620 0.0 10.329709 5 TGTAGGA 1655 0.0 10.115644 2 GGACGTG 1605 0.0 10.072817 6 GTATAGA 360 0.0 9.906149 1 ACGTGAA 1005 0.0 9.877857 8 TGGCGAG 1580 0.0 9.873172 18 TCGAACT 125 2.734254E-6 9.832482 19 TAGGACG 1710 0.0 9.78604 4 GTCCTAA 800 0.0 9.782322 1 >>END_MODULE