FastQCFastQC Report
Thu 26 May 2016
SRR1512077_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512077_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2252709
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT81010.3596114722318773No Hit
TATCAACGCAGAGTACTTTTTTTTT54200.24059920744312738No Hit
GGTATCAACGCAGAGTACTTTTTTT51380.22808094609645543No Hit
GTCCTACAGTGGACATTTCTAAATT27440.12180889764279364No Hit
GTCCTAAAGTGTGTATTTCTCATTT27370.12149816065901099No Hit
CTTTAGGACGTGAAATATGGCGAGG26760.11879030980033374No Hit
CTGTAGGACGTGGAATATGGCAAGA26380.11710345188837085No Hit
ACGCAGAGTACTTTTTTTTTTTTTT23330.10356419759498453No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCA500.001504223313.2949349
TACACCG853.934927E-612.2970785
ACCGTCC752.0810979E-411.3961658
GGTATCA18350.010.9694581
CCGTCCA700.001496601310.8530089
TAGGACC6400.010.5416014
GGCGAGG7850.010.52511919
AGGACGT15700.010.4706745
GTCCTAA9550.010.1890951
GGACGTG15600.010.166456
TAGGACG17100.010.1135464
TGTAGGA16700.010.0712862
GACGTGG7450.010.07043657
TTAGGAC10400.09.9591283
GTAGGAC16650.09.9303193
TGGCGAG16500.09.89947818
CCAACGA1651.0280928E-89.78458619
CCTACAC3400.09.7817673
CTGTAGG16700.09.6540371
CTAGAAC2201.6370905E-119.5022873
Produced by FastQC (version 0.11.5)