FastQCFastQC Report
Thu 26 May 2016
SRR1512076_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512076_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1852496
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT46680.25198434976377815No Hit
TATCAACGCAGAGTACTTTTTTTTT28870.15584379129563572No Hit
GGTATCAACGCAGAGTACTTTTTTT26790.14461569687599865No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAATT350.002240994716.20786314
TTACACG602.6881778E-514.1814954
ACCGTCC552.04225E-413.7517558
GGCGAGG4050.012.13954919
TCCAACG1900.011.44533418
GGCGTGC752.1691427E-411.3451988
TACACGG752.1691427E-411.3451985
GGTATCA10500.011.2260791
AGGACGT7850.011.2006725
GTCTAAA1457.521521E-910.9300581
GGACGTG7900.010.8904316
ACCGTGC1053.6727615E-610.804958
TTAGGAC6000.010.7149093
TAGGACG8450.010.6291294
TTATACC1356.1045284E-810.5048124
GACGTGA4600.010.4819767
GTATTAA2650.010.4660451
TCGAAAA1002.529294E-510.40004414
ATCCCGT1553.965397E-910.369266510
TCCCGTT1651.0095391E-910.31381511
Produced by FastQC (version 0.11.5)