FastQCFastQC Report
Thu 26 May 2016
SRR1512076_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512076_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1852496
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT57860.31233535726932743No Hit
TATCAACGCAGAGTACTTTTTTTTT38130.20583040395228924No Hit
GGTATCAACGCAGAGTACTTTTTTT35150.18974399944723228No Hit
ACGCAGAGTACTTTTTTTTTTTTTT18550.10013516898282102No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCTC550.003075692612.08730318
GCAGCGT550.003076936412.086658
CTACGAG658.0120756E-411.6945023
GGTATCA10950.011.5857191
GGACCGT752.0810238E-411.3959846
TAGGACC3650.010.9335584
TTAGACT1659.258656E-1010.36558154
GTATCAA22800.010.2498311
GATATAC4900.09.927951
GACGTGA3550.09.897927
TCCAACG1651.0270014E-89.78495918
GGTCTAC2159.094947E-129.7604471
GTAGGAC7600.09.7518293
GTCCTAA4650.09.6411841
TTAGGAC4100.09.5017823
ATTGGAC1401.0557724E-69.5017823
CTTACAC2700.09.5017813
ACACCGT800.00452928529.4966546
GACCGTG900.00111732089.4966537
TGTAGGA8050.09.3247312
Produced by FastQC (version 0.11.5)