FastQCFastQC Report
Thu 26 May 2016
SRR1512075_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512075_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2095642
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT67030.31985424991482325No Hit
GGTATCAACGCAGAGTACTTTTTTT41020.1957395394824116No Hit
TATCAACGCAGAGTACTTTTTTTTT38900.18562330779780134No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCTA400.005446566314.18339512
GGTATCA11650.013.0793791
TAGGACC5000.012.2922764
GTAGGAC12100.011.1747973
CCAATAC1451.3169483E-911.0858733
GTACCGT600.00608531311.031536
ACCGTCC600.00608531311.031538
GTCCTAC8800.011.0188351
TTCAACG650.00787345510.6555782
CTTAGAC2050.010.608723
TGTAGGA13400.010.4851792
GTATCAA23950.010.452191
TAGGACA5500.010.1432774
AGGACGT10600.09.9016155
GACGTGG5750.09.8667117
TCCAACG1252.7283331E-69.83405518
AGGATAG1356.870705E-79.8058045
TTAGGAC7500.09.70774653
TCCTACA10550.09.6599992
TGGCGAG10000.09.64493818
Produced by FastQC (version 0.11.5)