##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512075_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2095642 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.002193599861045 31.0 29.0 33.0 22.0 34.0 2 26.419135520284478 31.0 25.0 34.0 10.0 34.0 3 28.120815959977897 31.0 27.0 34.0 16.0 34.0 4 32.421249430961964 35.0 32.0 37.0 22.0 37.0 5 32.83158335250009 35.0 33.0 37.0 26.0 37.0 6 33.08690224761672 36.0 35.0 37.0 26.0 37.0 7 33.13908291587972 37.0 35.0 37.0 26.0 37.0 8 33.1784488953743 37.0 35.0 37.0 27.0 37.0 9 34.726713818486175 39.0 35.0 39.0 27.0 39.0 10 34.58638975550213 39.0 35.0 39.0 27.0 39.0 11 34.601263479162945 39.0 35.0 39.0 27.0 39.0 12 34.49520242484165 39.0 35.0 39.0 27.0 39.0 13 34.483423218278695 39.0 35.0 39.0 27.0 39.0 14 35.545613706921316 40.0 36.0 41.0 27.0 41.0 15 35.47142450857542 40.0 36.0 41.0 27.0 41.0 16 35.43219547995316 39.0 36.0 41.0 27.0 41.0 17 35.373020773586326 39.0 36.0 41.0 26.0 41.0 18 35.32849599311333 39.0 36.0 41.0 26.0 41.0 19 35.355103113986075 40.0 36.0 41.0 26.0 41.0 20 35.276342524152504 40.0 35.0 41.0 25.0 41.0 21 35.2065896751449 39.0 35.0 41.0 25.0 41.0 22 35.089868403095565 39.0 35.0 41.0 25.0 41.0 23 34.975953430977235 39.0 35.0 41.0 25.0 41.0 24 34.857933750134805 39.0 35.0 41.0 24.0 41.0 25 34.72669759434102 39.0 35.0 41.0 23.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6729126844845439 1101 2 3.091217158408334 1101 3 1.614074112411565 1101 4 0.8542374138128821 1101 5 0.4368986081307469 1101 6 0.21559638297454597 1101 7 0.08121420958993752 1101 8 0.027498177902863574 1101 9 -0.13069189607933396 1101 10 -0.3206448816280556 1101 11 -0.20755015744246919 1101 12 -0.2739941241038437 1101 13 -0.23239343584182848 1101 14 -0.21925815554329375 1101 15 -0.07555326987485245 1101 16 -0.03525655801792027 1101 17 -0.2191989331244173 1101 18 -0.15924547159065838 1101 19 -0.1402189249652821 1101 20 -0.166723496110059 1101 21 -0.2303332742271138 1101 22 -0.36427360293344435 1101 23 -0.3590352752926833 1101 24 -0.42551809063163404 1101 25 -0.47088636270131445 1102 1 1.240543955332143 1102 2 3.7236781753907664 1102 3 2.4396162420514216 1102 4 1.4862858354700847 1102 5 1.1376457468398442 1102 6 0.9001565799207043 1102 7 0.7319224450515307 1102 8 0.6748202354305803 1102 9 0.6825798793078022 1102 10 0.7111028682424774 1102 11 0.7490357819175557 1102 12 0.630709364425222 1102 13 0.7675491297107584 1102 14 0.6802737782549144 1102 15 0.5931164845069787 1102 16 0.6970616091850417 1102 17 0.6459177398011278 1102 18 0.6569277251763239 1102 19 0.733053439493105 1102 20 0.909733043930828 1102 21 0.7900628271029646 1102 22 1.000850924275511 1102 23 0.6458508253255957 1102 24 0.7438320410503394 1102 25 0.6601358477394825 1103 1 0.777693196406549 1103 2 2.8286161940852033 1103 3 1.7698827473729537 1103 4 0.9470503827350001 1103 5 0.7411311829004816 1103 6 0.48681178091926824 1103 7 0.454967485117848 1103 8 0.17172259939090395 1103 9 0.35531525105270845 1103 10 0.18224225710013542 1103 11 0.40276514133289965 1103 12 0.3013871092775773 1103 13 0.27056863405087483 1103 14 0.1885090499531401 1103 15 0.1062097375488733 1103 16 0.11388898577030915 1103 17 0.24222982829957118 1103 18 0.16826539704224075 1103 19 0.25117618248745543 1103 20 0.42899203145834264 1103 21 0.5812815863334606 1103 22 0.43640275322425737 1103 23 0.30634286446766623 1103 24 0.25738550885912304 1103 25 0.19128419315781997 1104 1 1.2317592585743178 1104 2 3.7940177679551503 1104 3 2.399965903779403 1104 4 1.5347956228385868 1104 5 1.1201539354043888 1104 6 0.7590411429732384 1104 7 0.7324284971612229 1104 8 0.7272760973580574 1104 9 0.8110572189734668 1104 10 0.8220431206082566 1104 11 0.7866491779139437 1104 12 0.8528596378489937 1104 13 0.6984958904820502 1104 14 0.7865296293350497 1104 15 0.6296680199954707 1104 16 0.7531315226656972 1104 17 0.788383670892209 1104 18 0.9319816671241767 1104 19 0.8242100255986031 1104 20 0.6894164430163485 1104 21 0.663336154195143 1104 22 0.8585837825266864 1104 23 0.8521816645561842 1104 24 0.9052626640415156 1104 25 0.7885462168556856 1105 1 1.0235981425242606 1105 2 3.5240394233382197 1105 3 2.0662500679955933 1105 4 1.4113268863732031 1105 5 1.0286802295984643 1105 6 0.6952468919075656 1105 7 0.3233510164801032 1105 8 0.3174967543139928 1105 9 0.46602815516986595 1105 10 0.4305281555987932 1105 11 0.24484858979933222 1105 12 0.19501149907218007 1105 13 0.23152285713275944 1105 14 0.2796990656112399 1105 15 0.518321739022575 1105 16 0.2747299178878322 1105 17 0.541533503347928 1105 18 0.2655648351927411 1105 19 0.4691618138773421 1105 20 0.5702584954907621 1105 21 0.49042407505042007 1105 22 0.5514584776416882 1105 23 0.5409739780349554 1105 24 0.7547844220027216 1105 25 0.6513016933880849 1106 1 0.651862056477917 1106 2 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0.3224921242722374 1107 14 0.5570884610857405 1107 15 0.5481934040006209 1107 16 0.6494681819877997 1107 17 0.5157415411729858 1107 18 0.3437006001370193 1107 19 0.4250854883116517 1107 20 0.5737910858318642 1107 21 0.5763815218589272 1107 22 0.6680812402218947 1107 23 0.6306497632426513 1107 24 0.708911406129701 1107 25 0.6633989578257768 1108 1 0.7023173543469206 1108 2 2.4368144643891263 1108 3 1.475741036796908 1108 4 0.9756126006589199 1108 5 0.8614224299268756 1108 6 0.5958544452737442 1108 7 0.5008551215704173 1108 8 0.5951486426555377 1108 9 0.5010363653504939 1108 10 0.44295836906348995 1108 11 0.6742902975168974 1108 12 0.7356026320771107 1108 13 0.7368102134545964 1108 14 0.674362448205656 1108 15 0.609766730812396 1108 16 0.5279975697761756 1108 17 0.5145220090950531 1108 18 0.7443168877379023 1108 19 0.7132504838280838 1108 20 0.647664078413591 1108 21 0.6959413164297246 1108 22 0.7800955876581028 1108 23 0.7516964739298615 1108 24 0.89864320689599 1108 25 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-1.6390597169910563 2315 18 -1.836501347212419 2315 19 -1.564809130432586 2315 20 -1.6905445714987408 2315 21 -1.7761578458535396 2315 22 -1.7003534674753453 2315 23 -1.785079290730181 2315 24 -1.6400057313467542 2315 25 -1.6958088796546917 2316 1 -1.1986395208840541 2316 2 -1.8717498437288675 2316 3 -0.9209026034604584 2316 4 -1.3954233982418778 2316 5 -1.5854947792344163 2316 6 -1.468650960720705 2316 7 -1.4313327663487705 2316 8 -1.5916866948282333 2316 9 -1.836101158220778 2316 10 -1.9911226118804919 2316 11 -2.265485881994337 2316 12 -2.134891535513461 2316 13 -2.124662908187382 2316 14 -2.509467895484825 2316 15 -2.8501252440575513 2316 16 -2.5312803675123945 2316 17 -2.559134262521816 2316 18 -2.458567383234609 2316 19 -2.6027036907746393 2316 20 -2.5814401658369164 2316 21 -2.7937528966451026 2316 22 -2.7856333450725614 2316 23 -2.778549046343798 2316 24 -2.6317774277990296 2316 25 -3.0327064721204913 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 82688.0 3 1.0 4 643.0 5 3447.0 6 4107.0 7 3771.0 8 3447.0 9 3415.0 10 3256.0 11 3179.0 12 3178.0 13 3171.0 14 3203.0 15 3326.0 16 3478.0 17 3749.0 18 4348.0 19 5497.0 20 7118.0 21 9162.0 22 11384.0 23 13434.0 24 15594.0 25 17968.0 26 20637.0 27 23771.0 28 27222.0 29 33282.0 30 41345.0 31 53486.0 32 68541.0 33 90103.0 34 120420.0 35 163602.0 36 236657.0 37 328592.0 38 675420.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.14286505042369 18.920788951548023 12.90897968259846 25.027366315429827 2 16.080287338271106 21.73667283014515 39.324008055298535 22.859031776285203 3 18.515328476905886 25.02937047453716 30.15014014798329 26.305160900573664 4 12.19225421135862 17.02981711570965 38.94696708693565 31.830961585996082 5 12.947869912895428 37.91806997569241 36.01483459484015 13.119225516572012 6 30.88600056689072 37.78932661208355 18.381145252862847 12.943527568162882 7 27.171196225309473 32.219577580521864 22.75226398402017 17.856962210148488 8 24.890570056875415 36.730668012958375 20.9286645132998 17.45009741686641 9 26.1346642222288 15.825269774131268 20.4763027272788 37.563763276361136 10 14.621247331366712 29.07404986156987 34.78294479686893 21.52175801019449 11 33.76235063049891 23.162400829912745 24.695725701240956 18.37952283834739 12 22.721390390152514 25.045976364283597 32.00551430062959 20.227118944934297 13 28.30526397161347 22.2684027138223 26.27318979100438 23.153143523559844 14 21.00596812514405 21.975078651653224 28.15688624868954 28.86206697451319 15 22.855382741899618 30.143459617625528 24.225368645980563 22.775788994494288 16 22.160607584692425 28.54786265974818 27.329333922492488 21.962195833066907 17 21.503243397488696 28.553827418996185 27.856380049645885 22.08654913386924 18 21.309221708669707 27.984216769849045 29.389895793270032 21.316665728211213 19 22.774433524922042 27.356481448343818 28.312563972256623 21.55652105447752 20 22.79632531603771 27.4542430078506 28.725599293006994 21.023832383104697 21 22.77144239606268 26.944157340151303 28.05331836891677 22.23108189486925 22 22.568637041455414 27.57029525797793 28.27123173277662 21.589835967790037 23 22.249241397936945 27.436175548080744 28.203915670268287 22.110667383714024 24 22.307637982241157 27.11067592019973 28.750992540675078 21.830693556884036 25 22.351241290258546 27.340977132544587 28.455194160071233 21.852587417125633 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 755.0 1 755.0 2 811.0 3 867.0 4 867.0 5 867.0 6 2095.0 7 3323.0 8 3323.0 9 3323.0 10 4879.5 11 6436.0 12 6436.0 13 6436.0 14 9947.0 15 13458.0 16 13458.0 17 13458.0 18 22254.5 19 31051.0 20 31051.0 21 31051.0 22 45468.5 23 59886.0 24 59886.0 25 59886.0 26 82432.0 27 104978.0 28 104978.0 29 104978.0 30 131338.0 31 157698.0 32 157698.0 33 157698.0 34 178433.5 35 199169.0 36 199169.0 37 199169.0 38 215593.5 39 232018.0 40 232018.0 41 232018.0 42 245185.0 43 258352.0 44 258352.0 45 258352.0 46 262160.5 47 265969.0 48 265969.0 49 265969.0 50 258239.5 51 250510.0 52 250510.0 53 250510.0 54 225689.0 55 200868.0 56 200868.0 57 200868.0 58 171282.5 59 141697.0 60 141697.0 61 141697.0 62 115424.0 63 89151.0 64 89151.0 65 89151.0 66 68087.5 67 47024.0 68 47024.0 69 47024.0 70 33906.0 71 20788.0 72 20788.0 73 20788.0 74 14247.0 75 7706.0 76 7706.0 77 7706.0 78 5225.5 79 2745.0 80 2745.0 81 2745.0 82 1794.0 83 843.0 84 843.0 85 843.0 86 547.0 87 251.0 88 251.0 89 251.0 90 160.0 91 69.0 92 69.0 93 69.0 94 46.0 95 23.0 96 23.0 97 23.0 98 15.0 99 7.0 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.437065109403228 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 4.2946266585609566E-4 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 4.2946266585609566E-4 15 0.0 16 0.0 17 0.0 18 0.0 19 3.340265178880744E-4 20 2.8630844390406377E-4 21 1.9087229593604252E-4 22 8.112072577281807E-4 23 5.248988138241169E-4 24 4.771807398401063E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2095642.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.24589496046029 #Duplication Level Percentage of deduplicated Percentage of total 1 83.31156842988644 61.85541958636548 2 11.452618039050625 17.00619751899251 3 2.9179676335277143 6.499413552507647 4 1.0058452304000876 2.9871951729105954 5 0.4560934823637059 1.693153439186313 6 0.2340251911881995 1.0425245857836418 7 0.15764718111191137 0.8193259234753343 8 0.09532071626916153 0.5661737510140804 9 0.0732380906237473 0.48938648251998723 >10 0.2740515277791072 3.494230624665563 >50 0.011558057715873867 0.580922970884492 >100 0.00811975270095786 1.1441815727885523 >500 0.0012977782549937673 0.5968210613658227 >1k 5.840002147469904E-4 0.9021209536706246 >5k 6.48889127496656E-5 0.322932803869383 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6703 0.31985424991482325 No Hit GGTATCAACGCAGAGTACTTTTTTT 4102 0.1957395394824116 No Hit TATCAACGCAGAGTACTTTTTTTTT 3890 0.18562330779780134 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTCGCTA 40 0.0054465663 14.183395 12 GGTATCA 1165 0.0 13.079379 1 TAGGACC 500 0.0 12.292276 4 GTAGGAC 1210 0.0 11.174797 3 CCAATAC 145 1.3169483E-9 11.085873 3 GTACCGT 60 0.006085313 11.03153 6 ACCGTCC 60 0.006085313 11.03153 8 GTCCTAC 880 0.0 11.018835 1 TTCAACG 65 0.007873455 10.655578 2 CTTAGAC 205 0.0 10.60872 3 TGTAGGA 1340 0.0 10.485179 2 GTATCAA 2395 0.0 10.45219 1 TAGGACA 550 0.0 10.143277 4 AGGACGT 1060 0.0 9.901615 5 GACGTGG 575 0.0 9.866711 7 TCCAACG 125 2.7283331E-6 9.834055 18 AGGATAG 135 6.870705E-7 9.805804 5 TTAGGAC 750 0.0 9.7077465 3 TCCTACA 1055 0.0 9.659999 2 TGGCGAG 1000 0.0 9.644938 18 >>END_MODULE