FastQCFastQC Report
Thu 26 May 2016
SRR1512075_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512075_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2095642
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT85070.4059376553819784No Hit
TATCAACGCAGAGTACTTTTTTTTT52690.251426531821752No Hit
GGTATCAACGCAGAGTACTTTTTTT52640.25118794145183193No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25510.12172880673321113No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT400.00529035214.2457596
GGTATCA15400.012.5717961
TCCAACG1301.9826984E-1012.41937818
CTATACT2900.011.4681254
CCAACGA752.080216E-411.39660619
GCCGGTT1001.9390372E-611.39579111
ACCGTCC700.001495486710.8539118
TAGGACC5300.010.7571634
GTCCTAA6300.010.7483011
GTCTTAG2350.010.5518241
GTCTAGG1909.094947E-1210.5411431
GGACGTG9650.010.5305436
TATACTG3100.010.4217245
TACACCG1201.5197365E-610.2940075
AGGACGT10000.010.1673135
TAGGACG10550.010.0876024
TGCACCG856.5812125E-410.06111055
TTAGGAC6450.010.0177823
TGTAGGA11650.09.9507612
CGGTTTC1054.122757E-59.94870713
Produced by FastQC (version 0.11.5)