##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512075_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2095642 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.567470016348214 31.0 31.0 34.0 27.0 34.0 2 30.750337128192697 31.0 31.0 34.0 27.0 34.0 3 30.85373694552791 31.0 31.0 34.0 27.0 34.0 4 34.45580208833379 37.0 35.0 37.0 31.0 37.0 5 34.039002367770834 37.0 35.0 37.0 30.0 37.0 6 33.974049002644534 37.0 35.0 37.0 30.0 37.0 7 33.791592743417056 37.0 35.0 37.0 29.0 37.0 8 33.68694509844716 37.0 35.0 37.0 28.0 37.0 9 35.13320738943006 38.0 35.0 39.0 29.0 39.0 10 34.93273994317732 38.0 35.0 39.0 28.0 39.0 11 34.92703333870957 38.0 35.0 39.0 28.0 39.0 12 34.725373894968705 38.0 35.0 39.0 27.0 39.0 13 34.6909472133122 38.0 35.0 39.0 27.0 39.0 14 35.64169548043034 39.0 36.0 41.0 27.0 41.0 15 35.558662691432986 39.0 35.0 41.0 27.0 41.0 16 35.45055357737629 39.0 35.0 41.0 27.0 41.0 17 35.392595204715306 39.0 35.0 41.0 27.0 41.0 18 35.31691004475001 39.0 35.0 40.0 27.0 41.0 19 35.33290514314945 39.0 35.0 40.0 27.0 41.0 20 35.216296485754725 39.0 35.0 40.0 26.0 41.0 21 35.08997529158129 39.0 34.0 40.0 26.0 41.0 22 34.95936901436409 39.0 34.0 40.0 25.0 41.0 23 35.01872743531577 39.0 35.0 40.0 26.0 41.0 24 34.88480522913742 39.0 35.0 41.0 25.0 41.0 25 34.42141978448609 38.0 34.0 40.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1503574982363105 1101 2 0.08025901476591102 1101 3 0.06164551123521633 1101 4 0.12175341145528762 1101 5 0.2591670691429826 1101 6 0.07236932510748062 1101 7 0.09898621270482977 1101 8 0.0757168126107004 1101 9 0.1405314469279091 1101 10 0.15761958359354367 1101 11 0.19647077005588898 1101 12 0.051510370498220936 1101 13 0.1843926160099869 1101 14 -0.011723827961859001 1101 15 0.12811995384762298 1101 16 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0.2045452412131894 1103 3 0.2534904947985481 1103 4 0.2599243104075981 1103 5 0.2255815237742098 1103 6 0.27815246901676005 1103 7 0.2595041870287389 1103 8 0.42178603455054997 1103 9 0.37095586089873933 1103 10 0.113602704565686 1103 11 0.06016471744891305 1103 12 0.22031775548249755 1103 13 0.1989360081413949 1103 14 0.29294580511155743 1103 15 0.20729978767872836 1103 16 0.3297674750292856 1103 17 0.3260525695533971 1103 18 0.3920422916852644 1103 19 0.3846516787678951 1103 20 0.3876287576585753 1103 21 0.40039541863666983 1103 22 0.46137004528436165 1103 23 0.39920062336192785 1103 24 0.4744876853770421 1103 25 0.39726520700796897 1104 1 0.25021002492425737 1104 2 0.2931772761979552 1104 3 0.18020804356879339 1104 4 0.3165400641068601 1104 5 0.32451088851333765 1104 6 0.3019943116612467 1104 7 0.26428607391343206 1104 8 0.23880397785308105 1104 9 0.4879755171436173 1104 10 0.2915906084149924 1104 11 0.656678139046619 1104 12 0.6412244945680499 1104 13 0.3106842000613099 1104 14 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-0.7413180356999973 2315 2 -0.7886251026874085 2315 3 -0.8244436428272586 2315 4 -0.7899560292311278 2315 5 -0.8897186106387949 2315 6 -1.0244456360876555 2315 7 -1.0336981209409686 2315 8 -0.9935460639315679 2315 9 -1.2856303765205297 2315 10 -1.2229895978307965 2315 11 -1.0852947455547266 2315 12 -1.2916261516741727 2315 13 -1.3912969197673348 2315 14 -1.3450799628456949 2315 15 -1.4820037234513208 2315 16 -1.4972929780409032 2315 17 -1.6154884264142098 2315 18 -1.4738602424917033 2315 19 -1.4796059352372808 2315 20 -1.5253904535665512 2315 21 -1.4414504653747358 2315 22 -1.4870145398384125 2315 23 -1.473964311234674 2315 24 -1.3965127080268829 2315 25 -1.592562842692061 2316 1 -1.2704093068165747 2316 2 -1.2399738388367503 2316 3 -1.261607070105196 2316 4 -1.1844919186773808 2316 5 -1.362226889594183 2316 6 -1.4648811720190196 2316 7 -1.7113435441108678 2316 8 -1.716464797426056 2316 9 -1.9702769314969828 2316 10 -2.1107252162023897 2316 11 -2.0297202489291877 2316 12 -2.1124629200171228 2316 13 -2.0996698430420295 2316 14 -2.279696476043121 2316 15 -2.251870771112955 2316 16 -2.4871147534401743 2316 17 -2.285892732868305 2316 18 -2.2830722396412284 2316 19 -2.165811868139322 2316 20 -2.2115108023306433 2316 21 -2.4386185037164267 2316 22 -2.417577159481546 2316 23 -2.3587491981238813 2316 24 -2.3496108538203373 2316 25 -2.5618674803043646 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 38424.0 3 0.0 4 722.0 5 6009.0 6 7605.0 7 6000.0 8 5012.0 9 4379.0 10 4130.0 11 3944.0 12 3906.0 13 3637.0 14 3639.0 15 3626.0 16 3716.0 17 3962.0 18 4293.0 19 4878.0 20 5832.0 21 7464.0 22 9816.0 23 12241.0 24 15076.0 25 17830.0 26 20380.0 27 23369.0 28 27438.0 29 33532.0 30 42359.0 31 54791.0 32 69801.0 33 89965.0 34 116388.0 35 154020.0 36 221278.0 37 379302.0 38 686878.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.9900253869619 19.909454127609493 13.672877160263702 24.42764332516491 2 15.278993263162317 21.501716419121205 39.88605878294098 23.3332315347755 3 18.701047220851652 25.542244333717306 30.60174400016797 25.154964445263072 4 12.895952648400824 17.846034771206153 38.499180680669696 30.758831899723333 5 12.974733554323025 38.224755397067675 35.952775747344134 12.847735301265159 6 30.918305702977893 38.270563388212295 18.049981819413812 12.761149089395992 7 27.208082296499114 32.42762838309215 22.65267636361554 17.711612956793193 8 25.054388257958056 36.90341723846513 20.722272248125936 17.319922255450876 9 26.121064571143354 15.635160967378969 20.37432920317497 37.86944525830271 10 14.547904651653287 29.403161417837588 34.716855264401076 21.33207866610805 11 34.07676501997956 23.111724235341725 24.5168306418749 18.294680102803817 12 22.779558722339026 25.213419085893484 31.90368393074771 20.103338261019772 13 28.494132108442187 22.254516754292958 26.26665241486857 22.98469872239629 14 21.004255497837896 21.999893111514275 28.158053713372798 28.837797677275027 15 22.83715443763773 30.308898180128097 24.1891506278267 22.66479675440748 16 22.155835777294023 28.76354835415591 27.224211005505712 21.856404863044357 17 21.572243732469573 28.600400259204577 27.917125157827527 21.91023085049832 18 21.434720243247654 27.982355764963675 29.435609708146714 21.147314283641958 19 22.789160929758484 27.420106783556918 28.36158483251664 21.42914745416796 20 22.927293879393524 27.44891541589642 28.675317635359477 20.948473069350584 21 22.848081876580064 26.998695387857275 28.039808326040422 22.113414409522235 22 22.6262405506284 27.585675415934592 28.33332219911607 21.454761834320937 23 22.266350836640992 27.396473252587988 28.37340538126264 21.96377052950838 24 22.39144927252676 27.116434371840015 28.83823318178933 21.65388317384389 25 22.435068173109546 27.391832778923288 28.496278668709657 21.676820379257506 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 698.0 1 698.0 2 788.5 3 879.0 4 879.0 5 879.0 6 2141.0 7 3403.0 8 3403.0 9 3403.0 10 5007.5 11 6612.0 12 6612.0 13 6612.0 14 9907.5 15 13203.0 16 13203.0 17 13203.0 18 22140.5 19 31078.0 20 31078.0 21 31078.0 22 46032.0 23 60986.0 24 60986.0 25 60986.0 26 83943.5 27 106901.0 28 106901.0 29 106901.0 30 133231.0 31 159561.0 32 159561.0 33 159561.0 34 179928.5 35 200296.0 36 200296.0 37 200296.0 38 217017.0 39 233738.0 40 233738.0 41 233738.0 42 246669.0 43 259600.0 44 259600.0 45 259600.0 46 262530.5 47 265461.0 48 265461.0 49 265461.0 50 257973.5 51 250486.0 52 250486.0 53 250486.0 54 224772.5 55 199059.0 56 199059.0 57 199059.0 58 169462.5 59 139866.0 60 139866.0 61 139866.0 62 113379.5 63 86893.0 64 86893.0 65 86893.0 66 66331.5 67 45770.0 68 45770.0 69 45770.0 70 33087.5 71 20405.0 72 20405.0 73 20405.0 74 13899.0 75 7393.0 76 7393.0 77 7393.0 78 4910.0 79 2427.0 80 2427.0 81 2427.0 82 1579.5 83 732.0 84 732.0 85 732.0 86 445.0 87 158.0 88 158.0 89 158.0 90 96.0 91 34.0 92 34.0 93 34.0 94 18.0 95 2.0 96 2.0 97 2.0 98 1.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.41724683891618897 2 0.0 3 0.0 4 0.0 5 0.05368283323201196 6 0.0 7 0.0 8 0.0029108025130246485 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 4.771807398401063E-5 20 0.0 21 0.0 22 0.0 23 0.0 24 0.004819525472385073 25 0.001002079553664223 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2095642.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.2507507066642 #Duplication Level Percentage of deduplicated Percentage of total 1 82.17847850157493 59.374567636702544 2 12.04427741889718 17.404161704692903 3 3.15782079969483 6.844647701252106 4 1.1394580143131594 3.2930678773140287 5 0.5081328436099287 1.8356489704764678 6 0.27783058620700046 1.2044081053637026 7 0.16589413214795024 0.8390182909863974 8 0.11052152612172586 0.6388210585232714 9 0.07965616770249703 0.5179696123429216 >10 0.3128065536734923 3.8221928224798045 >50 0.01368517030462693 0.6707777770166017 >100 0.009192116682090008 1.261454263968278 >500 0.0015855311086605934 0.7897668665577469 >1k 4.624465733592256E-4 0.594687863558632 >5k 1.9819138858252527E-4 0.9088094487644557 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 8507 0.4059376553819784 No Hit TATCAACGCAGAGTACTTTTTTTTT 5269 0.251426531821752 No Hit GGTATCAACGCAGAGTACTTTTTTT 5264 0.25118794145183193 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2551 0.12172880673321113 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 4.771807398401063E-5 0.0 12 0.0 0.0 0.0 4.771807398401063E-5 0.0 13 0.0 0.0 0.0 4.771807398401063E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGACCGT 40 0.005290352 14.245759 6 GGTATCA 1540 0.0 12.571796 1 TCCAACG 130 1.9826984E-10 12.419378 18 CTATACT 290 0.0 11.468125 4 CCAACGA 75 2.080216E-4 11.396606 19 GCCGGTT 100 1.9390372E-6 11.395791 11 ACCGTCC 70 0.0014954867 10.853911 8 TAGGACC 530 0.0 10.757163 4 GTCCTAA 630 0.0 10.748301 1 GTCTTAG 235 0.0 10.551824 1 GTCTAGG 190 9.094947E-12 10.541143 1 GGACGTG 965 0.0 10.530543 6 TATACTG 310 0.0 10.421724 5 TACACCG 120 1.5197365E-6 10.294007 5 AGGACGT 1000 0.0 10.167313 5 TAGGACG 1055 0.0 10.087602 4 TGCACCG 85 6.5812125E-4 10.0611105 5 TTAGGAC 645 0.0 10.017782 3 TGTAGGA 1165 0.0 9.950761 2 CGGTTTC 105 4.122757E-5 9.948707 13 >>END_MODULE