FastQCFastQC Report
Thu 26 May 2016
SRR1512074_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512074_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1988397
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT66110.3324788762002759No Hit
GGTATCAACGCAGAGTACTTTTTTT41320.2078055840961337No Hit
TATCAACGCAGAGTACTTTTTTTTT41150.2069506240453994No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13300.012.4842651
CTAGGAC2500.012.48308853
GGCGAGG6350.011.318192519
TAGGACC5050.010.8613734
GTATCAA27450.010.333441
TGGCGAG13550.010.32903218
TATGGCG14700.010.03562616
GGACCGG951.721559E-49.9546166
CGTTAGG800.00325689869.8833771
GCGTACA800.00325689869.8833771
ACACCGT1252.7241604E-69.835166
TAGGACT2600.09.8206124
ATGGCGA15000.09.7718717
CCTAGAC1752.7830538E-99.7270823
AGGACGT13300.09.6701985
TCTATAC2253.6379788E-129.6670393
TAGGACA5400.09.6320134
GTCCTAG1958.221832E-109.6299571
GGACGTG13450.09.5623526
ACCGTCC900.00116083649.4571238
Produced by FastQC (version 0.11.5)