FastQCFastQC Report
Thu 26 May 2016
SRR1512074_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512074_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1988397
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT81050.4076147771295169No Hit
TATCAACGCAGAGTACTTTTTTTTT58690.29516238457410665No Hit
GGTATCAACGCAGAGTACTTTTTTT54770.2754480116395267No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25910.13030597008545072No Hit
GTACTTTTTTTTTTTTTTTTTTTTT22820.11476581386916193No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTATCG400.00527024214.2538885
GGTATCA15900.013.3143371
GCCGGTT752.0812423E-411.39594111
TCTATAC2700.010.9103833
CTTAGAC1451.4377292E-810.4856193
GGCGAGG6050.010.36020819
TGGCGAG12950.010.04686718
TAGGACC4450.010.0364464
GTATCAA31200.09.5359451
AGGACCT10200.09.5025925
TACACCG800.0045062339.5025925
CTTATAC1804.1618478E-99.5025913
TCCAACG900.00111717779.49685718
TCGTCAA1304.2582233E-69.49661810
ACGAAAT900.00111743479.49661712
ATGGCGA14250.09.39665317
GTCTAGG1951.5716068E-99.2914331
TATGGCG14150.09.2617216
TTAATAC2401.0913936E-119.106653
GTATATG3800.09.0340531
Produced by FastQC (version 0.11.5)