##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512074_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1988397 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.062784242784513 31.0 30.0 34.0 26.0 34.0 2 30.26262059337245 31.0 30.0 34.0 26.0 34.0 3 30.349981920109517 31.0 30.0 34.0 26.0 34.0 4 33.95994109828168 37.0 35.0 37.0 30.0 37.0 5 33.47431272527569 35.0 35.0 37.0 28.0 37.0 6 33.396493758540174 36.0 35.0 37.0 28.0 37.0 7 33.18342363220222 36.0 35.0 37.0 27.0 37.0 8 33.058544646768226 35.0 33.0 37.0 26.0 37.0 9 34.4306886401458 38.0 35.0 39.0 27.0 39.0 10 34.195445879268576 38.0 34.0 39.0 27.0 39.0 11 34.18609161047819 38.0 34.0 39.0 26.0 39.0 12 33.97006432820005 37.0 34.0 39.0 25.0 39.0 13 33.92554806711134 37.0 34.0 39.0 25.0 39.0 14 34.78202692922993 38.0 34.0 40.0 25.0 41.0 15 34.692325023624555 38.0 34.0 40.0 25.0 41.0 16 34.562455586082656 38.0 33.0 40.0 24.0 41.0 17 34.4955484241829 38.0 33.0 40.0 24.0 41.0 18 34.40347928507235 38.0 33.0 40.0 23.0 41.0 19 34.418686509786525 38.0 34.0 40.0 22.0 41.0 20 34.28252557210658 38.0 34.0 40.0 21.0 41.0 21 34.13893301991504 38.0 34.0 40.0 19.0 41.0 22 33.99187888535338 38.0 33.0 40.0 18.0 41.0 23 34.04041547035124 38.0 34.0 40.0 18.0 41.0 24 33.88430077092251 38.0 34.0 40.0 18.0 41.0 25 33.401435427633416 38.0 33.0 40.0 15.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.0764625466084965 1101 2 -0.19092577938952005 1101 3 -0.05327004298933957 1101 4 -0.2428700300204838 1101 5 -0.17758222110617794 1101 6 -0.5018836584943855 1101 7 -0.29426947818060967 1101 8 -0.33994920975914056 1101 9 -0.4315044424169727 1101 10 -0.23702585898686834 1101 11 -0.20179835454641193 1101 12 -0.41117829913022064 1101 13 -0.36920566031000845 1101 14 -0.40380684801866096 1101 15 -0.3089009769794444 1101 16 -0.43096915004378644 1101 17 -0.4831073364508782 1101 18 -0.4580778339677707 1101 19 -0.6782530753309999 1101 20 -0.5129591202903043 1101 21 -0.6888929721981754 1101 22 -1.3574151494847015 1101 23 -0.9249339129825032 1101 24 -1.1900730065703868 1101 25 -1.199794170378567 1102 1 0.24962621498044513 1102 2 0.2875708763675924 1102 3 0.2902011855206119 1102 4 0.32160176033798393 1102 5 0.28353615867460746 1102 6 0.3005405410679174 1102 7 0.4888548972704072 1102 8 0.4505344645468341 1102 9 0.5215575010550424 1102 10 0.522522619110859 1102 11 0.370049236511683 1102 12 0.4946909879246064 1102 13 0.6674190052329578 1102 14 0.8175719465870657 1102 15 0.5849481568701549 1102 16 0.5416267865734099 1102 17 0.7605934772472267 1102 18 0.7452264639769623 1102 19 0.7114361530244082 1102 20 0.7877539951401005 1102 21 0.9101905205875056 1102 22 0.9333342569072727 1102 23 0.9265581288064553 1102 24 0.9524088970614599 1102 25 0.8341687214256481 1103 1 0.20217921253433602 1103 2 0.17926718994090152 1103 3 0.3013937198016201 1103 4 0.37531731957925274 1103 5 0.29286549019843733 1103 6 0.25700639246706203 1103 7 0.42819260104140255 1103 8 0.38683290685811045 1103 9 0.3655117730652293 1103 10 0.4096048487153183 1103 11 0.19754252834147934 1103 12 0.20572450329263603 1103 13 0.13500247863730408 1103 14 0.24772284424101798 1103 15 0.38595861462385983 1103 16 0.4917057581981794 1103 17 0.49043266300072474 1103 18 0.4132188431575514 1103 19 0.45830846946820003 1103 20 0.6717900483055317 1103 21 0.43093942821285935 1103 22 0.44844822473007895 1103 23 0.47342952325301013 1103 24 0.5557733674367924 1103 25 0.3570046722493885 1104 1 0.16817845140651855 1104 2 0.18515743693220443 1104 3 0.3123621243768042 1104 4 0.1311068477078976 1104 5 -0.003530663393199518 1104 6 0.17919235561701896 1104 7 0.14590342672817513 1104 8 0.15639600884188098 1104 9 0.4265340929736894 1104 10 0.1422512232038997 1104 11 0.4682416173967354 1104 12 0.45126454021316675 1104 13 0.2383300168857545 1104 14 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0.3026597463421581 1107 11 0.29832497460862584 1107 12 0.28593135525780866 1107 13 0.07726506826612223 1107 14 0.198118955040961 1107 15 0.3371690896334414 1107 16 0.3243384848766624 1107 17 0.30562111092721267 1107 18 0.3339589148383837 1107 19 0.3266831204505607 1107 20 0.462851197701994 1107 21 0.2470733186743317 1107 22 0.33883662881567744 1107 23 0.2324633747859437 1107 24 0.24066107330196473 1107 25 0.1775357344799957 1108 1 -0.28441261878773716 1108 2 -0.2539254662177157 1108 3 -0.394918524480385 1108 4 -0.24803424558749043 1108 5 -0.3326436888713147 1108 6 -0.30344302950565805 1108 7 -0.3108366410561487 1108 8 -0.3684263837766082 1108 9 -0.590451236897664 1108 10 -0.35961952043941636 1108 11 -0.3413861405964198 1108 12 -0.3400698331355656 1108 13 -0.19238511524134339 1108 14 -0.46921982930197004 1108 15 -0.30805279293922894 1108 16 -0.45980441293966834 1108 17 -0.39388331629808704 1108 18 -0.4044775435040435 1108 19 -0.18540025485081202 1108 20 -0.27262583561712717 1108 21 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-1.201770609774897 2315 13 -1.391156772496764 2315 14 -1.4755630005541178 2315 15 -1.5064932558438642 2315 16 -1.5759949287211938 2315 17 -1.471541767669848 2315 18 -1.3969269975358642 2315 19 -1.5610721796655795 2315 20 -1.621222964870178 2315 21 -1.399786266319559 2315 22 -1.5110140190854224 2315 23 -1.5237377530153609 2315 24 -1.4218249054407224 2315 25 -1.6018435080046771 2316 1 -1.3412451121342919 2316 2 -1.4722395161336088 2316 3 -1.5829514167848338 2316 4 -1.4017036309543016 2316 5 -1.5851375151155587 2316 6 -1.9991163546721822 2316 7 -1.9702329004638592 2316 8 -2.014908100997097 2316 9 -2.2975719746077345 2316 10 -2.2815032388754233 2316 11 -2.1350987524356384 2316 12 -2.2339260354238704 2316 13 -2.315712929114092 2316 14 -2.4327210197043527 2316 15 -2.298513961293942 2316 16 -2.6750077135740824 2316 17 -2.7076483592417198 2316 18 -2.4871959148000684 2316 19 -2.3781678306521314 2316 20 -2.421346034263408 2316 21 -2.4186523255010393 2316 22 -2.673031151267093 2316 23 -2.5031812630112036 2316 24 -2.6739278581308277 2316 25 -2.68367977783166 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 50265.0 3 3.0 4 883.0 5 7567.0 6 9378.0 7 7506.0 8 6023.0 9 5567.0 10 5022.0 11 4702.0 12 4395.0 13 4433.0 14 4197.0 15 4179.0 16 4264.0 17 4586.0 18 4864.0 19 5498.0 20 6677.0 21 8582.0 22 11024.0 23 13492.0 24 16397.0 25 19355.0 26 22082.0 27 25118.0 28 29198.0 29 35522.0 30 45150.0 31 56997.0 32 72344.0 33 92594.0 34 118555.0 35 156559.0 36 223566.0 37 367102.0 38 534751.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.235544243452615 20.624236180549243 14.22678746376592 23.913432112232222 2 15.555394621898946 22.171528120390445 39.28134069805979 22.991736559650814 3 18.460498582526526 26.382759579701638 30.96876529184061 24.18797654593122 4 13.456819739719986 18.633552555148693 37.991759190946276 29.91786851418504 5 13.217906127883374 38.32370070174132 35.66230673935337 12.796086431021939 6 30.76528479976584 38.23547309717325 18.233883877314238 12.76535822574667 7 27.015178558406593 32.54873146559766 23.021308119052684 17.414781856943055 8 25.143613270660847 36.50814806022755 20.98026178805689 17.367976881054716 9 26.363799583282415 15.574656368924314 20.63214740315943 37.42939664463385 10 15.22271457862791 29.276497600831224 34.270520424241234 21.23026739629963 11 33.534399820558974 23.073058348005958 24.954071043156876 18.438470788278195 12 22.960304204844405 25.363295156852477 31.84318825667108 19.833212381632038 13 28.59318335322373 22.30661180840647 26.36143587020097 22.73876896816883 14 21.406992667963188 22.19586933595253 28.036352901357226 28.36078509472706 15 23.01829061299127 30.55989322051884 24.039565539477277 22.382250627012613 16 22.242439512833705 28.370290238820516 27.475398524540118 21.91187172380566 17 21.633858832013928 28.659317027736414 27.989179223263765 21.717644916985893 18 21.477652601567996 27.69255837742664 29.704480543875295 21.12530847713007 19 23.12870430673986 27.315447886360605 28.268528134500436 21.287319672399097 20 23.198787767231593 27.369685228855207 28.55973932770971 20.871787676203496 21 22.92565317690582 26.869734766246378 28.085186207784464 22.11942584906334 22 23.076830230582726 27.447184842865884 28.22338798539728 21.252596941154106 23 22.497318191487917 27.48756913232116 28.25290925303146 21.76220342315946 24 22.752577718832626 27.009152090063367 28.629892334565042 21.60837785653896 25 22.648583567695447 27.333864420009093 28.382810896932973 21.634741115362488 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1323.0 1 1323.0 2 1206.0 3 1089.0 4 1089.0 5 1089.0 6 2387.5 7 3686.0 8 3686.0 9 3686.0 10 5062.0 11 6438.0 12 6438.0 13 6438.0 14 9367.0 15 12296.0 16 12296.0 17 12296.0 18 20846.5 19 29397.0 20 29397.0 21 29397.0 22 44440.0 23 59483.0 24 59483.0 25 59483.0 26 81905.5 27 104328.0 28 104328.0 29 104328.0 30 129275.5 31 154223.0 32 154223.0 33 154223.0 34 172681.0 35 191139.0 36 191139.0 37 191139.0 38 206066.0 39 220993.0 40 220993.0 41 220993.0 42 232845.5 43 244698.0 44 244698.0 45 244698.0 46 246951.0 47 249204.0 48 249204.0 49 249204.0 50 241670.0 51 234136.0 52 234136.0 53 234136.0 54 210413.0 55 186690.0 56 186690.0 57 186690.0 58 158915.5 59 131141.0 60 131141.0 61 131141.0 62 106597.5 63 82054.0 64 82054.0 65 82054.0 66 63448.0 67 44842.0 68 44842.0 69 44842.0 70 32461.5 71 20081.0 72 20081.0 73 20081.0 74 13846.5 75 7612.0 76 7612.0 77 7612.0 78 5084.0 79 2556.0 80 2556.0 81 2556.0 82 1659.0 83 762.0 84 762.0 85 762.0 86 475.5 87 189.0 88 189.0 89 189.0 90 111.0 91 33.0 92 33.0 93 33.0 94 17.5 95 2.0 96 2.0 97 2.0 98 2.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.41324745511082545 2 0.0 3 0.0 4 0.0 5 0.05305781491321904 6 0.0 7 0.0 8 0.0035707155060081064 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 1.0058353538051004E-4 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0054315109105475415 25 0.0010561271214953552 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1988397.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.63851284379642 #Duplication Level Percentage of deduplicated Percentage of total 1 83.98566179455499 61.00593572958525 2 10.886941282599885 15.816224483715786 3 2.704064629354807 5.892576999295343 4 0.9631601250496571 2.7985007645620836 5 0.4563058610088761 1.6572689572796415 6 0.2605930173017627 1.1357453540566653 7 0.1511217672325445 0.7684082301068877 8 0.11568779647473865 0.6722711592080649 9 0.07557678512244753 0.49408067491287727 >10 0.36901802017413654 4.5399697771927645 >50 0.01775882848713409 0.8861012372737507 >100 0.01141391227662824 1.591466922899185 >500 0.0017283207451408273 0.8482665683585234 >1k 7.604611278619227E-4 0.9164111180425087 >5k 2.07398489416888E-4 0.9767720235105944 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 8105 0.4076147771295169 No Hit TATCAACGCAGAGTACTTTTTTTTT 5869 0.29516238457410665 No Hit GGTATCAACGCAGAGTACTTTTTTT 5477 0.2754480116395267 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2591 0.13030597008545072 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2282 0.11476581386916193 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.0058353538051004E-4 0.0 10 0.0 0.0 0.0 1.0058353538051004E-4 0.0 11 0.0 0.0 0.0 1.0058353538051004E-4 0.0 12 0.0 0.0 0.0 1.0058353538051004E-4 0.0 13 0.0 0.0 0.0 1.0058353538051004E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTATCG 40 0.005270242 14.253888 5 GGTATCA 1590 0.0 13.314337 1 GCCGGTT 75 2.0812423E-4 11.395941 11 TCTATAC 270 0.0 10.910383 3 CTTAGAC 145 1.4377292E-8 10.485619 3 GGCGAGG 605 0.0 10.360208 19 TGGCGAG 1295 0.0 10.046867 18 TAGGACC 445 0.0 10.036446 4 GTATCAA 3120 0.0 9.535945 1 AGGACCT 1020 0.0 9.502592 5 TACACCG 80 0.004506233 9.502592 5 CTTATAC 180 4.1618478E-9 9.502591 3 TCCAACG 90 0.0011171777 9.496857 18 TCGTCAA 130 4.2582233E-6 9.496618 10 ACGAAAT 90 0.0011174347 9.496617 12 ATGGCGA 1425 0.0 9.396653 17 GTCTAGG 195 1.5716068E-9 9.291433 1 TATGGCG 1415 0.0 9.26172 16 TTAATAC 240 1.0913936E-11 9.10665 3 GTATATG 380 0.0 9.034053 1 >>END_MODULE