FastQCFastQC Report
Thu 26 May 2016
SRR1512073_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512073_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2643748
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT66410.25119640752446903No Hit
TATCAACGCAGAGTACTTTTTTTTT37640.14237363016444834No Hit
GGTATCAACGCAGAGTACTTTTTTT36430.13779679455076654No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12200.012.0753091
TCCAACG2450.011.96560618
TTACACG658.342928E-411.638564
GTATCAA29150.011.5322011
GTATAAG3100.010.8439891
TGCACCG1159.362702E-710.6897655
GTCCTAC12300.010.3694671
GTCCTAA6600.010.0369721
TTAGGAC7700.09.9475683
GTCTTAA3600.09.8871671
GTATAAC1905.0931703E-109.8871661
AGGACGT12200.09.7663745
CTAACAC1857.057679E-109.7119063
TAGGACC5650.09.7073834
GGACGTG11900.09.6947246
TAGGACG12500.09.6832824
CCAACGA2151.0913936E-119.67661119
GTCTAAC1552.3881148E-79.5682261
AATCCCG2305.456968E-129.45668819
GAACCGT1002.8869844E-49.4563316
Produced by FastQC (version 0.11.5)