##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512073_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2643748 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.068745962171885 31.0 30.0 33.0 22.0 34.0 2 26.522899308103497 31.0 25.0 34.0 10.0 34.0 3 28.20299438524398 31.0 27.0 34.0 16.0 34.0 4 32.50564009882939 35.0 32.0 37.0 23.0 37.0 5 32.90144332969708 35.0 33.0 37.0 26.0 37.0 6 33.15691775464227 36.0 35.0 37.0 27.0 37.0 7 33.20684989643491 37.0 35.0 37.0 27.0 37.0 8 33.24659233784763 37.0 35.0 37.0 27.0 37.0 9 34.79582358076488 39.0 35.0 39.0 28.0 39.0 10 34.6645977604522 39.0 35.0 39.0 27.0 39.0 11 34.682650540066604 39.0 35.0 39.0 27.0 39.0 12 34.57167362396113 39.0 35.0 39.0 27.0 39.0 13 34.55891673487791 39.0 35.0 39.0 27.0 39.0 14 35.635799251668466 40.0 36.0 41.0 27.0 41.0 15 35.563958440819626 40.0 36.0 41.0 27.0 41.0 16 35.52918621593283 40.0 36.0 41.0 27.0 41.0 17 35.4609395449188 39.0 36.0 41.0 27.0 41.0 18 35.41238045381027 39.0 36.0 41.0 27.0 41.0 19 35.433966096617375 40.0 36.0 41.0 26.0 41.0 20 35.35881596884423 40.0 36.0 41.0 26.0 41.0 21 35.288648539875965 39.0 36.0 41.0 26.0 41.0 22 35.18046576300011 39.0 35.0 41.0 25.0 41.0 23 35.07336327062942 39.0 35.0 41.0 25.0 41.0 24 34.94716213496899 39.0 35.0 41.0 24.0 41.0 25 34.81939768843324 39.0 35.0 41.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8724645119157763 1101 2 3.212112277121882 1101 3 1.7038043702132377 1101 4 1.073723592709598 1101 5 0.7561077671684089 1101 6 0.39199705883929425 1101 7 0.2747160989917461 1101 8 0.40412406395423517 1101 9 0.2780184242295647 1101 10 0.13573670423129158 1101 11 0.4278765921090013 1101 12 0.2287731015419041 1101 13 0.17490292273776475 1101 14 0.3557380351764863 1101 15 0.43048292239878805 1101 16 0.31751932100503666 1101 17 0.15237462101341492 1101 18 0.21510395044557384 1101 19 0.3500861116672098 1101 20 0.36726608569307473 1101 21 0.42007126883014223 1101 22 0.23650783570960243 1101 23 0.3233734559564141 1101 24 0.1647315422607818 1101 25 0.027993650386854085 1102 1 1.0203525644406994 1102 2 3.660189134069256 1102 3 2.1652303431088775 1102 4 1.2512535541838972 1102 5 0.9462310636598303 1102 6 0.7950802715204048 1102 7 0.613840146076349 1102 8 0.6414631399775885 1102 9 0.5076548786313211 1102 10 0.5886529899689563 1102 11 0.6622669515518353 1102 12 0.522519907370679 1102 13 0.4332889932460873 1102 14 0.4706456398830525 1102 15 0.6289909554059108 1102 16 0.48413381373804043 1102 17 0.34113116795276 1102 18 0.3993210687132134 1102 19 0.32259104151105333 1102 20 0.5478405147738741 1102 21 0.6139607229366462 1102 22 0.5513677427688606 1102 23 0.32631387213839247 1102 24 0.2324223298856012 1102 25 0.1979388262545143 1103 1 0.9209357235006372 1103 2 2.813742384662138 1103 3 1.7695465433533855 1103 4 1.092657321992185 1103 5 0.8333448160199666 1103 6 0.7546238854076321 1103 7 0.5911040362788569 1103 8 0.36206304329525807 1103 9 0.4996540659393176 1103 10 0.5005258246877133 1103 11 0.5156097604536143 1103 12 0.5426195847459638 1103 13 0.5386534610904619 1103 14 0.6362136835722509 1103 15 0.47601175611772106 1103 16 0.44400189140617385 1103 17 0.5020901620362253 1103 18 0.47892941772239084 1103 19 0.7016120278809481 1103 20 0.7101998262275799 1103 21 0.863004846507863 1103 22 0.783796781094992 1103 23 0.790517766754661 1103 24 0.6033264672585759 1103 25 0.634311960478307 1104 1 1.2814312463770499 1104 2 3.8186618877749794 1104 3 2.350230007435087 1104 4 1.5697683365631008 1104 5 1.2009779540472962 1104 6 0.8739552203012195 1104 7 0.6376614102006926 1104 8 0.774762385058807 1104 9 0.7164856457964106 1104 10 0.7124315424972707 1104 11 0.7180990521521124 1104 12 0.6708503932642742 1104 13 0.5186647279665664 1104 14 0.8725116967809114 1104 15 0.5466082155206919 1104 16 0.7252714469368726 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-2.0364244271508056 2315 20 -2.005707585681236 2315 21 -2.060787545480906 2315 22 -2.1662602543639053 2315 23 -1.934319507427027 2315 24 -2.103494592329973 2315 25 -2.0990084776514593 2316 1 -1.0480264437563456 2316 2 -1.6411293497571613 2316 3 -0.75149326185117 2316 4 -1.068073217787557 2316 5 -1.1896739135752021 2316 6 -1.2086302935352151 2316 7 -1.2269976543249115 2316 8 -1.449499665858692 2316 9 -1.526366269286079 2316 10 -1.7927626616432306 2316 11 -2.0105125585303867 2316 12 -1.7843322021413286 2316 13 -1.8384391771298496 2316 14 -2.096892752055318 2316 15 -2.3969120859271484 2316 16 -2.006349209333358 2316 17 -2.0352576087849386 2316 18 -1.8045762539447097 2316 19 -2.023751138997831 2316 20 -2.118145221666012 2316 21 -2.1037850279138084 2316 22 -2.1323043216480215 2316 23 -2.0548077708315873 2316 24 -2.1009671653098394 2316 25 -2.378782159544045 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 102141.0 3 4.0 4 810.0 5 4317.0 6 5006.0 7 4548.0 8 4299.0 9 4119.0 10 3946.0 11 3953.0 12 3889.0 13 3926.0 14 3945.0 15 4057.0 16 4354.0 17 4650.0 18 5209.0 19 6702.0 20 8652.0 21 11251.0 22 13631.0 23 16442.0 24 19191.0 25 22090.0 26 25020.0 27 28813.0 28 33779.0 29 41238.0 30 51369.0 31 66076.0 32 85848.0 33 113232.0 34 152108.0 35 208334.0 36 300926.0 37 417444.0 38 858429.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.06110113369353 18.680713895575522 13.255612864766233 25.002572105964717 2 16.273908285674622 21.35520130160577 39.0659697243777 23.304920688341905 3 18.721791940835512 24.364122450399964 29.797734125945436 27.11635148281909 4 12.5294468307872 16.86166760220717 38.126591490565666 32.48229407643996 5 13.547849492462975 37.42380136079536 35.522523326731594 13.505825820010076 6 31.720156384042653 36.933323448377074 18.219777376663735 13.126742790916532 7 27.874971442058776 31.810293568070787 22.193189366006138 18.121545623864304 8 25.20016174074804 36.23522309518685 20.716848915001755 17.84776624906335 9 26.053050442023974 16.121317160334495 20.308970446502467 37.51666195113906 10 14.989855311474468 29.02587538600502 33.99382240667416 21.990446895846354 11 34.04969006123125 23.030939408748488 24.379328135661947 18.540042394358313 12 23.124859101548257 24.73605653791511 31.36603791284192 20.773046447694714 13 28.231472893785643 21.97240432900564 26.18188269078596 23.61424008642276 14 21.345842370397715 21.718042078236078 27.747261268308332 29.18885428305788 15 23.098759791023955 29.77545514928049 23.94116231955542 23.184622740140135 16 22.624584491411436 28.383756696931776 26.79540561354562 22.196253198111165 17 21.939618276635397 28.22145992033277 27.61236230244422 22.226559500587616 18 21.65187453569705 27.873571913813265 28.874234609350058 21.600318941139623 19 23.096539823537736 27.254137891122344 27.765913263719778 21.883409021620146 20 23.159862044026987 27.25224322191802 28.188340617200925 21.399554116854066 21 23.08994365566409 26.77831136448915 27.611674957938437 22.520070021908325 22 22.944522679896465 27.290126938124143 27.764721724503534 22.00062865747586 23 22.66714931825463 27.094115924892453 27.754433226703647 22.48430153014927 24 22.62243838080778 26.91420254200681 28.20075211688904 22.26260696029637 25 22.74075393749856 27.043283642496807 27.946206652905897 22.269755767098744 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 437.0 1 437.0 2 648.5 3 860.0 4 860.0 5 860.0 6 2027.5 7 3195.0 8 3195.0 9 3195.0 10 4684.5 11 6174.0 12 6174.0 13 6174.0 14 9857.5 15 13541.0 16 13541.0 17 13541.0 18 23234.5 19 32928.0 20 32928.0 21 32928.0 22 49869.0 23 66810.0 24 66810.0 25 66810.0 26 93742.5 27 120675.0 28 120675.0 29 120675.0 30 154240.0 31 187805.0 32 187805.0 33 187805.0 34 214434.0 35 241063.0 36 241063.0 37 241063.0 38 265677.5 39 290292.0 40 290292.0 41 290292.0 42 308912.5 43 327533.0 44 327533.0 45 327533.0 46 334241.5 47 340950.0 48 340950.0 49 340950.0 50 333428.5 51 325907.0 52 325907.0 53 325907.0 54 295651.5 55 265396.0 56 265396.0 57 265396.0 58 227686.0 59 189976.0 60 189976.0 61 189976.0 62 155240.5 63 120505.0 64 120505.0 65 120505.0 66 92482.5 67 64460.0 68 64460.0 69 64460.0 70 46532.0 71 28604.0 72 28604.0 73 28604.0 74 19798.5 75 10993.0 76 10993.0 77 10993.0 78 7493.0 79 3993.0 80 3993.0 81 3993.0 82 2604.0 83 1215.0 84 1215.0 85 1215.0 86 765.0 87 315.0 88 315.0 89 315.0 90 204.5 91 94.0 92 94.0 93 94.0 94 56.5 95 19.0 96 19.0 97 19.0 98 13.5 99 8.0 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.3566557780847495 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 4.1607596488016255E-4 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 2.269505262982705E-4 15 0.0 16 0.0 17 3.7825087716378416E-5 18 0.0 19 3.7825087716378416E-4 20 2.269505262982705E-4 21 1.5130035086551366E-4 22 7.186766666111899E-4 23 7.186766666111899E-4 24 3.7825087716378416E-4 25 3.7825087716378416E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2643748.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.16756530464458 #Duplication Level Percentage of deduplicated Percentage of total 1 85.08876498315115 63.9591529856257 2 10.20763812867102 15.345666112861178 3 2.4328814222981903 5.486213195671674 4 0.895632533209096 2.692900677158359 5 0.4335515094714196 1.6294505700560082 6 0.249073665519882 1.123335661117976 7 0.15277551338206485 0.8038634365367874 8 0.10110669108614465 0.6079955043963444 9 0.08132270193600803 0.5501546557672528 >10 0.3306499701184315 4.164769072179924 >50 0.016234984490233532 0.8262932132659985 >100 0.00874723169353096 1.2387844754046937 >500 9.622698276708631E-4 0.4874761067495464 >1k 6.07749364804407E-4 0.831126775633354 >5k 5.064578040036726E-5 0.25281755757516966 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6641 0.25119640752446903 No Hit TATCAACGCAGAGTACTTTTTTTTT 3764 0.14237363016444834 No Hit GGTATCAACGCAGAGTACTTTTTTT 3643 0.13779679455076654 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.7825087716378416E-5 0.0 5 0.0 0.0 0.0 3.7825087716378416E-5 0.0 6 0.0 0.0 0.0 3.7825087716378416E-5 0.0 7 0.0 0.0 0.0 3.7825087716378416E-5 0.0 8 0.0 0.0 0.0 3.7825087716378416E-5 0.0 9 0.0 0.0 0.0 3.7825087716378416E-5 0.0 10 0.0 0.0 0.0 3.7825087716378416E-5 0.0 11 0.0 0.0 0.0 3.7825087716378416E-5 0.0 12 0.0 0.0 0.0 3.7825087716378416E-5 3.7825087716378416E-5 13 0.0 0.0 0.0 3.7825087716378416E-5 3.7825087716378416E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1220 0.0 12.075309 1 TCCAACG 245 0.0 11.965606 18 TTACACG 65 8.342928E-4 11.63856 4 GTATCAA 2915 0.0 11.532201 1 GTATAAG 310 0.0 10.843989 1 TGCACCG 115 9.362702E-7 10.689765 5 GTCCTAC 1230 0.0 10.369467 1 GTCCTAA 660 0.0 10.036972 1 TTAGGAC 770 0.0 9.947568 3 GTCTTAA 360 0.0 9.887167 1 GTATAAC 190 5.0931703E-10 9.887166 1 AGGACGT 1220 0.0 9.766374 5 CTAACAC 185 7.057679E-10 9.711906 3 TAGGACC 565 0.0 9.707383 4 GGACGTG 1190 0.0 9.694724 6 TAGGACG 1250 0.0 9.683282 4 CCAACGA 215 1.0913936E-11 9.676611 19 GTCTAAC 155 2.3881148E-7 9.568226 1 AATCCCG 230 5.456968E-12 9.456688 19 GAACCGT 100 2.8869844E-4 9.456331 6 >>END_MODULE