FastQCFastQC Report
Thu 26 May 2016
SRR1512073_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512073_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2643748
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT81080.3066858112043962No Hit
TATCAACGCAGAGTACTTTTTTTTT52490.19854388542327028No Hit
GGTATCAACGCAGAGTACTTTTTTT47960.18140912068775086No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGCT604.08873E-412.6684954
GGTATCA14950.012.3742611
ATACCGC550.00307654912.0871666
TCCAACG1104.987687E-711.22464618
CATACCG600.005871192611.0849335
TAGGACC5550.010.4429494
ATTAGAC1553.6579877E-910.4208583
AATCCCG2250.010.13115519
GTATCAA30400.010.0690891
ACCGTGC1252.5817426E-69.8769428
AGGACGT10600.09.8599155
GTAGGAC13100.09.6464313
GTCCTAC13850.09.501411
TGGACCG1401.0571239E-69.5013725
CCAACGA1201.705606E-59.49795619
AACCGTC800.00452876849.4970597
TAGGACA6150.09.4241254
TAGGACG12050.09.3042484
GGACGTG10800.09.2332526
TGTAGGA15300.09.1287692
Produced by FastQC (version 0.11.5)