FastQCFastQC Report
Thu 26 May 2016
SRR1512072_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512072_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2197965
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT75820.344955447425232No Hit
TATCAACGCAGAGTACTTTTTTTTT47450.21588150857725216No Hit
GGTATCAACGCAGAGTACTTTTTTT44340.2017320566979001No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG500.001097550813.8620721
TTAGGAC3500.011.3457853
GGTATCA17550.010.9452251
TAGGACC2700.010.85553554
TACCGTA700.001552332910.8055117
GTATTAG2001.0913936E-1110.3965541
TTAGACT2100.010.355284
GTAGGAC4700.010.2594873
TAGGACG4700.010.2594874
GTATCAA32700.09.5381221
TAGACTG2800.09.4548235
CTTACAC2750.09.2829163
GACGTGA2252.910383E-119.2447157
CTTAGAC2252.910383E-119.2447153
TCTTATA1853.3616743E-89.0986572
AGGACGT5000.09.0766295
TTTAGAC1151.1700238E-49.0437423
GTATAAG2207.348717E-109.0013451
GTCTAAC1000.0019958658.9113321
GTACCGT850.007733268.8986566
Produced by FastQC (version 0.11.5)