##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512072_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2197965 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.55345967747439 31.0 28.0 33.0 16.0 34.0 2 26.007879561321495 31.0 25.0 33.0 10.0 34.0 3 27.66960802378564 31.0 26.0 34.0 16.0 34.0 4 31.9320303098548 35.0 32.0 37.0 19.0 37.0 5 32.31275611759059 35.0 33.0 37.0 22.0 37.0 6 32.557540270204484 35.0 33.0 37.0 25.0 37.0 7 32.591036253989486 36.0 33.0 37.0 25.0 37.0 8 32.63082624154616 36.0 33.0 37.0 25.0 37.0 9 34.12478042189025 38.0 35.0 39.0 25.0 39.0 10 33.983707656855316 38.0 34.0 39.0 25.0 39.0 11 33.99169186042544 38.0 34.0 39.0 25.0 39.0 12 33.88352043822354 38.0 34.0 39.0 24.0 39.0 13 33.856207901399706 38.0 34.0 39.0 23.0 39.0 14 34.87482739716056 39.0 34.0 41.0 24.0 41.0 15 34.77645958875596 39.0 34.0 41.0 23.0 41.0 16 34.73577513745669 39.0 34.0 41.0 22.0 41.0 17 34.670388745953645 39.0 34.0 41.0 22.0 41.0 18 34.61789291458235 39.0 34.0 41.0 22.0 41.0 19 34.63182716740258 39.0 34.0 41.0 19.0 41.0 20 34.545914971348495 39.0 34.0 41.0 19.0 41.0 21 34.46903886094638 39.0 34.0 41.0 18.0 41.0 22 34.345757553009264 39.0 34.0 41.0 18.0 41.0 23 34.213661728007494 39.0 34.0 41.0 17.0 41.0 24 34.07658402203857 39.0 34.0 41.0 11.0 41.0 25 33.92909259246621 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.9362212854448337 1101 2 3.197521739309316 1101 3 1.8772845799051154 1101 4 1.1470190691804163 1101 5 0.9194156024486304 1101 6 0.3975533555313788 1101 7 0.4606230918597447 1101 8 0.3173496388692172 1101 9 0.4379673735569014 1101 10 0.1722954326893813 1101 11 0.42682608301110747 1101 12 0.2047690240553024 1101 13 0.21482468207676675 1101 14 0.36784270367171246 1101 15 0.48257077550433536 1101 16 0.34483158870971664 1101 17 0.23718249685398263 1101 18 0.16930056968243434 1101 19 0.34931811051000494 1101 20 0.3627866521812493 1101 21 0.5877419672428914 1101 22 0.32919081144426343 1101 23 0.3121688993602092 1101 24 0.26077786278577975 1101 25 0.2307176874591761 1102 1 1.2439681215821103 1102 2 3.7316992264093507 1102 3 2.4038989611408645 1102 4 1.3689976888267594 1102 5 1.1192752910212462 1102 6 0.940278413689775 1102 7 0.8108440709318643 1102 8 0.7850327635635708 1102 9 0.9251684119528747 1102 10 0.7139578717316084 1102 11 0.7999114261799534 1102 12 0.8474081586034501 1102 13 0.7022268182839753 1102 14 0.9165723596573443 1102 15 0.9098510647195468 1102 16 0.9959049482771718 1102 17 0.6259383869631705 1102 18 0.6115073439364309 1102 19 0.6275058535984073 1102 20 0.8703854072029813 1102 21 0.7366708928778323 1102 22 0.6681119216869931 1102 23 0.5906592032464246 1102 24 0.6942391674535315 1102 25 0.5939245576916719 1103 1 1.0285395127909638 1103 2 2.8181175298014693 1103 3 1.7745468326527458 1103 4 1.1103367991259745 1103 5 0.8607509526390729 1103 6 0.792863481380536 1103 7 0.7533842101252475 1103 8 0.6127388037132135 1103 9 0.7648869650070651 1103 10 0.709129648397699 1103 11 0.7484596009958864 1103 12 0.7639297116945585 1103 13 0.6287450843579947 1103 14 0.7762051656001248 1103 15 0.7245186779048041 1103 16 0.7896432919319167 1103 17 0.8045448429493831 1103 18 0.7251307176080033 1103 19 0.885723062711854 1103 20 0.9594404409427213 1103 21 1.1288865336526825 1103 22 0.7883235628870651 1103 23 0.7831002648951184 1103 24 0.7820863669728055 1103 25 0.7862023804676355 1104 1 1.3977923723008523 1104 2 3.835286652506902 1104 3 2.442308332476358 1104 4 1.727992891099781 1104 5 1.2903630921804137 1104 6 0.9363655259602623 1104 7 0.838845099672298 1104 8 0.8907266581727882 1104 9 0.9315337434001805 1104 10 0.918900724588049 1104 11 0.8146536440341094 1104 12 0.7083429652400497 1104 13 0.7529581118944506 1104 14 0.8395607281546162 1104 15 0.5342368056422941 1104 16 0.8967830517206963 1104 17 0.8232391680098488 1104 18 0.7986110415847207 1104 19 0.7282979224995287 1104 20 0.7701536344321482 1104 21 0.6233272721401377 1104 22 0.7656907075895774 1104 23 1.008127277083041 1104 24 0.9036597967610049 1104 25 0.8647297614037157 1105 1 1.3197215407372411 1105 2 3.8063352187484583 1105 3 2.3730742189609266 1105 4 1.6446153929697402 1105 5 1.2611303819152653 1105 6 1.028765798090582 1105 7 0.6194149696335955 1105 8 0.8032064394662086 1105 9 0.7992611169291166 1105 10 0.8562355764386496 1105 11 0.6237665016906533 1105 12 0.38976568561612623 1105 13 0.5537116071779025 1105 14 0.7490335445656271 1105 15 0.8229148311188013 1105 16 0.7387006433422911 1105 17 0.877400870052206 1105 18 0.8399380151873288 1105 19 1.0364003146653786 1105 20 1.2540281587992155 1105 21 1.0375279143777263 1105 22 1.108457140339432 1105 23 1.0699592453796072 1105 24 1.00509662481398 1105 25 0.938219356678772 1106 1 0.4836378407103936 1106 2 3.0406017854258387 1106 3 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0.4165725703642025 1107 15 0.34913630491482905 1107 16 0.5495753563952874 1107 17 0.33844025698653724 1107 18 0.27673523393902144 1107 19 0.3267989320888205 1107 20 0.5290466179667277 1107 21 0.49530681881858385 1107 22 0.7014307864827245 1107 23 0.6161928710417754 1107 24 0.7813894204071161 1107 25 0.8781704273104864 1108 1 0.49514968860346187 1108 2 2.174287474343835 1108 3 1.3652883240235205 1108 4 0.8298645991679123 1108 5 0.5010870120840778 1108 6 0.36805837177054457 1108 7 0.24634455709158942 1108 8 0.20937454102585207 1108 9 0.1963065178122534 1108 10 0.17236076629912844 1108 11 0.3110467598421707 1108 12 0.4539294243493046 1108 13 0.4394793248464097 1108 14 0.35799554662604294 1108 15 0.17883231096980268 1108 16 0.25896123823259387 1108 17 0.38502994287522796 1108 18 0.4020660031772181 1108 19 0.336285311781495 1108 20 0.39154412000130634 1108 21 0.3722227220973622 1108 22 0.3714296183520318 1108 23 0.5072732760838505 1108 24 0.5561724715065637 1108 25 0.40617360925987356 1109 1 0.7212002796527024 1109 2 3.1187541619773427 1109 3 1.4548697010095175 1109 4 1.0234892674717742 1109 5 0.4685919433805097 1109 6 0.26547829814869317 1109 7 0.30258661552958444 1109 8 -0.07838249792116159 1109 9 0.11205774403775592 1109 10 0.02432930711866277 1109 11 0.08515713760959187 1109 12 0.026627477332951344 1109 13 0.013928517808984964 1109 14 -0.040490008188484694 1109 15 -0.23212126383906195 1109 16 -0.2561283801337595 1109 17 -0.13289149988121096 1109 18 0.0105232973694811 1109 19 -0.27730662743548606 1109 20 -0.11003508446329135 1109 21 0.14147039176705078 1109 22 0.03133447946159862 1109 23 -0.027191894799599936 1109 24 -0.13464602332719267 1109 25 -0.04911758072719863 1110 1 0.24311463364253072 1110 2 1.5834563361134784 1110 3 1.0322084000463292 1110 4 0.5391223765011119 1110 5 0.34293845640626586 1110 6 0.3311839761185169 1110 7 0.3155097510942326 1110 8 0.3544919651419747 1110 9 0.31903389157994155 1110 10 0.07484095480012343 1110 11 0.1508320287561844 1110 12 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-2.036263605671067 2315 19 -1.9873899694970447 2315 20 -2.066374698943349 2315 21 -2.0428383941756607 2315 22 -2.2994173402028864 2315 23 -2.028097816343532 2315 24 -2.0432339827321186 2315 25 -2.0491617886103484 2316 1 -1.692755940228043 2316 2 -2.080526569869427 2316 3 -1.2736084497705207 2316 4 -1.8478739604952246 2316 5 -1.9864339489661376 2316 6 -2.1408013718668286 2316 7 -2.049110517526035 2316 8 -2.2082210995710874 2316 9 -2.5656571951111466 2316 10 -2.6409661270069584 2316 11 -2.8730800591559067 2316 12 -2.858129526669334 2316 13 -2.905042812747247 2316 14 -3.1365465302827396 2316 15 -3.2269171472009823 2316 16 -3.208026934409677 2316 17 -3.07144414220744 2316 18 -3.185418546949723 2316 19 -3.4115707783788842 2316 20 -3.3576688343611956 2316 21 -3.295211793971742 2316 22 -3.3846449373480105 2316 23 -3.527447387734341 2316 24 -3.2407100599195324 2316 25 -3.4847219260673903 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 106681.0 3 3.0 4 921.0 5 4414.0 6 5257.0 7 4822.0 8 4290.0 9 4267.0 10 4138.0 11 3995.0 12 3922.0 13 3837.0 14 3958.0 15 4090.0 16 4236.0 17 4748.0 18 5469.0 19 6653.0 20 8817.0 21 11078.0 22 13779.0 23 16265.0 24 19037.0 25 21775.0 26 24193.0 27 27466.0 28 31675.0 29 37816.0 30 47144.0 31 59249.0 32 74892.0 33 96938.0 34 127470.0 35 170670.0 36 240547.0 37 330236.0 38 663217.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.553241293651176 18.49224168719702 13.240747691614743 24.713769327537065 2 16.817394146523217 21.490875115124158 37.810942429106625 23.880788309246 3 18.39633479150032 24.63924584786382 29.849201420404786 27.11521794023108 4 12.938741062755776 16.987713635112478 37.28462464142969 32.78892066070206 5 14.093854997691047 37.125340940369846 34.78817906563571 13.992624996303398 6 32.018844704078546 36.28624659628338 17.95333410677604 13.741574592862033 7 28.10317725714468 31.89145414053454 21.981696705816518 18.02367189650427 8 26.207260213143872 34.5548492414757 20.597174096613575 18.640716448766852 9 26.746376762141345 15.566717395408936 20.0529125805006 37.63399326194912 10 15.997479486707022 28.21104976648855 33.10685111000403 22.684619636800406 11 33.87879242845086 23.02743674262329 23.778085638306344 19.315685190619504 12 23.301144467723553 24.739065453726518 30.578557893324053 21.38123218522588 13 28.400998196058623 21.673730018448882 25.89390640888276 24.031365376609727 14 22.369773385011626 21.13162359409215 27.23679307647966 29.261809944416566 15 23.82635756256355 28.48907967142334 24.005978257160603 23.678584508852506 16 23.49541507712816 27.746756659000482 25.799091432302152 22.9587368315692 17 22.559003441820046 27.57646277352005 26.85156497032482 23.01296881433508 18 22.45172238866406 27.1345995045417 27.986569394872074 22.427108711922163 19 23.641820760240968 26.867765169627667 27.464318065698162 22.0260960044332 20 23.813832996202848 26.49017589931036 27.546791072821097 22.149200031665696 21 23.902256815853217 26.17991013501586 27.284432320092595 22.63340072903833 22 23.873600687912173 26.109224256183587 27.25747342416018 22.759701631744054 23 23.592133225778714 26.27694950710957 27.104537722144194 23.026379544967522 24 23.3476041420226 26.326502757102038 27.336757720795646 22.989135380079713 25 23.505560825581846 26.211063415477497 27.308851596817966 22.97452416212269 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1040.0 1 1040.0 2 1075.0 3 1110.0 4 1110.0 5 1110.0 6 2323.0 7 3536.0 8 3536.0 9 3536.0 10 4656.0 11 5776.0 12 5776.0 13 5776.0 14 8529.0 15 11282.0 16 11282.0 17 11282.0 18 18640.0 19 25998.0 20 25998.0 21 25998.0 22 37967.5 23 49937.0 24 49937.0 25 49937.0 26 68657.5 27 87378.0 28 87378.0 29 87378.0 30 110536.5 31 133695.0 32 133695.0 33 133695.0 34 155630.0 35 177565.0 36 177565.0 37 177565.0 38 199371.5 39 221178.0 40 221178.0 41 221178.0 42 241633.0 43 262088.0 44 262088.0 45 262088.0 46 274153.5 47 286219.0 48 286219.0 49 286219.0 50 286333.0 51 286447.0 52 286447.0 53 286447.0 54 264702.5 55 242958.0 56 242958.0 57 242958.0 58 210067.0 59 177176.0 60 177176.0 61 177176.0 62 145845.0 63 114514.0 64 114514.0 65 114514.0 66 89064.5 67 63615.0 68 63615.0 69 63615.0 70 46556.0 71 29497.0 72 29497.0 73 29497.0 74 20405.0 75 11313.0 76 11313.0 77 11313.0 78 7661.5 79 4010.0 80 4010.0 81 4010.0 82 2586.0 83 1162.0 84 1162.0 85 1162.0 86 751.5 87 341.0 88 341.0 89 341.0 90 221.5 91 102.0 92 102.0 93 102.0 94 63.0 95 24.0 96 24.0 97 24.0 98 14.0 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.510717868573885 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 5.914561878828826E-4 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 3.639730386971585E-4 15 0.0 16 0.0 17 0.0 18 0.0 19 1.8198651934857925E-4 20 1.3648988951143443E-4 21 4.5496629837144813E-5 22 4.5496629837144816E-4 23 5.459595580457377E-4 24 2.2748314918572408E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2197965.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.95516994072489 #Duplication Level Percentage of deduplicated Percentage of total 1 80.40131573297157 56.24487705557903 2 12.687854210047906 17.75161994894086 3 3.483754436186056 7.311199008454494 4 1.4457101934092296 4.045396090599237 5 0.6417688868098009 2.2447525769724734 6 0.3793031711210903 1.5920530676899036 7 0.21317112240567615 1.04386954660409 8 0.15983237616826432 0.8944880829504638 9 0.10915952885707302 0.6872646052661404 >10 0.4578270623231454 5.2397249569776765 >50 0.013802768137373893 0.6622164102985933 >100 0.005319779373237983 0.7659417006433581 >500 4.591736292757661E-4 0.23416623996578592 >1k 6.559623275366337E-4 0.9345082259263748 >5k 6.559623275366338E-5 0.3479224831315381 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 7582 0.344955447425232 No Hit TATCAACGCAGAGTACTTTTTTTTT 4745 0.21588150857725216 No Hit GGTATCAACGCAGAGTACTTTTTTT 4434 0.2017320566979001 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 4.5496629837144813E-5 0.0 0.0 0.0 3 0.0 4.5496629837144813E-5 0.0 0.0 0.0 4 0.0 4.5496629837144813E-5 0.0 0.0 0.0 5 0.0 4.5496629837144813E-5 0.0 0.0 0.0 6 0.0 4.5496629837144813E-5 0.0 0.0 0.0 7 0.0 4.5496629837144813E-5 0.0 0.0 0.0 8 0.0 4.5496629837144813E-5 0.0 0.0 0.0 9 0.0 4.5496629837144813E-5 0.0 9.099325967428963E-5 0.0 10 0.0 4.5496629837144813E-5 0.0 1.3648988951143445E-4 0.0 11 0.0 4.5496629837144813E-5 0.0 1.8198651934857925E-4 0.0 12 0.0 4.5496629837144813E-5 0.0 1.8198651934857925E-4 0.0 13 0.0 4.5496629837144813E-5 0.0 2.2748314918572408E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATACG 50 0.0010975508 13.862072 1 TTAGGAC 350 0.0 11.345785 3 GGTATCA 1755 0.0 10.945225 1 TAGGACC 270 0.0 10.8555355 4 TACCGTA 70 0.0015523329 10.805511 7 GTATTAG 200 1.0913936E-11 10.396554 1 TTAGACT 210 0.0 10.35528 4 GTAGGAC 470 0.0 10.259487 3 TAGGACG 470 0.0 10.259487 4 GTATCAA 3270 0.0 9.538122 1 TAGACTG 280 0.0 9.454823 5 CTTACAC 275 0.0 9.282916 3 GACGTGA 225 2.910383E-11 9.244715 7 CTTAGAC 225 2.910383E-11 9.244715 3 TCTTATA 185 3.3616743E-8 9.098657 2 AGGACGT 500 0.0 9.076629 5 TTTAGAC 115 1.1700238E-4 9.043742 3 GTATAAG 220 7.348717E-10 9.001345 1 GTCTAAC 100 0.001995865 8.911332 1 GTACCGT 85 0.00773326 8.898656 6 >>END_MODULE