FastQCFastQC Report
Thu 26 May 2016
SRR1512072_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512072_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2197965
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT98800.4495067027909907No Hit
TATCAACGCAGAGTACTTTTTTTTT64550.2936807455987698No Hit
GGTATCAACGCAGAGTACTTTTTTT61760.2809871858742064No Hit
ACGCAGAGTACTTTTTTTTTTTTTT29580.13457903105827437No Hit
GTACTTTTTTTTTTTTTTTTTTTTT24340.11073879702361049No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20150.012.1231461
GTGTACG802.7403168E-511.9278021
GTATAAG2450.010.126461
GTATTAA3550.09.9454361
TCTACAC2900.09.8288223
TAAGACC2054.1836756E-119.7329324
GTGTTAG1803.8762664E-99.5422421
GAAGCGT900.00111725949.4968736
GTATCAA38350.09.4302731
AGCACCG1651.06381776E-79.213285
TAGGACG4050.09.1492994
AAGACCG1252.7505399E-59.1211475
GCACCGT1151.1148353E-49.0839656
GGTCTAC2451.4551915E-118.9580231
GTCTTAC1606.32368E-78.9458511
TAGGACC1401.0835491E-58.8225384
GATCTAC1304.1475898E-58.8082231
GTAGGAC4000.08.7886063
GGACGTG4000.08.7846076
TTATACT2502.5465852E-118.7410994
Produced by FastQC (version 0.11.5)